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Ube2i (BC037635) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200308
Ube2i (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ube2i
Synonyms Mmubc9, UBC9, 5830467E05Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200308 representing BC037635
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGGGATCGCCCTCAGCCGCCTTGCGCAGGAAAGGAAAGCCTGGAGGAAGGACCACCCTTTTGGCT
TTGTAGCTGTCCCAACAAAGAACCCTGATGGCACAATGAACCTGATGAACTGGGAGTGCGCTATCCCTGG
AAAGAAGGGGACTCCATGGGAAGGAGGCTTGTTCAAGCTACGGATGCTTTTCAAAGATGACTATCCGTCC
TCACCACCAAAATGTAAGTTGTGCAAAGAGCCTGCAGTCCACTTCCCAGCACTACCACCACCCTTTGGGA
GCCATGTCTGTGAGTGCTTGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200308 representing BC037635
Red=Cloning site Green=Tags(s)

MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPS
SPPKCKLCKEPAVHFPALPPPFGSHVCECLA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC037635
ORF Size 303 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037635, AAH37635
RefSeq Size 2252 bp
RefSeq ORF 305 bp
Locus ID 22196
Cytogenetics 17 A3.3
Summary Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity). Sumoylates p53/TP53 at 'Lys-386'.UniProtKB/Swiss-Prot Function
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"BC037635" in other vectors (4)
SKU Description Size Price
MC206861 Ube2i (untagged) - Mouse ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933), (10ug) 10 ug
$165.00
MR200308 Ube2i (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933) 10 ug
$289.00
MR200308L3 Lenti ORF clone of Ube2i (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933) 10 ug
$450.00
MR200308L4 Lenti ORF clone of Ube2i (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933) 10 ug
$450.00

Plasmid Map for MG200308

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG200308
Circular map for MG200308

file-alt Citations

Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization
Fuhs, Insel
J. Biol. Chem. (2011) 286 (17), 14830-41 DOI: 10.1074/jbc.M110.214270
Cited by 6
AI Summary: In this study, OriGene products including mammalian expression plasmids for eNOS, SUMO-1, SUMO-3, Ubc9, PIAS3, and PIASy were used to investigate the SUMOylation of Caveolin-3 and its impact on G-protein-coupled receptor desensitization.
Snippet: The mammalian expression plasmids for eNOS, SUMO-1, SUMO-3, Ubc9, PIAS3, and PIASy were obtained from Origene (catalog nos. ... 38 appeared to be the major site of SUMOylation;however, other sites can be modified, suggesting that two or more lysines might need to be
Showing 1-1 of 1 papers.
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