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Smim8 (NM_025471) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200284
Smim8 (tGFP-tagged) - Mouse RIKEN cDNA 1810030N24 gene (1810030N24Rik)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Smim8
Synonyms 1810030N24Rik; 2810406B13Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200284 representing NM_025471
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCAGCACCTGATCCTCCAACAGTTAAAAAAGAACCATTAAAAGAGAAAAACTTTGAAAACCCAG
GCCTCCGAGGGGCGCACACAACCACCTTATTTCGAGCTGTGAATCCCGAGCTCTTCATTAAACCCAACAA
ACCTGTGATGGCTTTTGGATTGGTAACCCTTTCACTTTGTGTGGCTTACATTGGTTATCTGCATGCAACT
CAAGAGAACAGAAAGGACCTCTATGAAGCCATTGACAGTGAAGGGCATCGGTACATGAGGAGAAAAACAT
CTAAGTGGGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200284 representing NM_025471
Red=Cloning site Green=Tags(s)

MSSAPDPPTVKKEPLKEKNFENPGLRGAHTTTLFRAVNPELFIKPNKPVMAFGLVTLSLCVAYIGYLHAT
QENRKDLYEAIDSEGHRYMRRKTSKWD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025471
ORF Size 291 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025471.3
RefSeq Size 930 bp
RefSeq ORF 294 bp
Locus ID 66291
UniProt ID Q9CQQ0
Cytogenetics 4 A5
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Documents
Resources
"NM_025471" in other vectors (4)
SKU Description Size Price
MC204688 Smim8 (untagged) - Mouse RIKEN cDNA 1810030N24 gene (1810030N24Rik), (10ug) 10 ug
$150.00
MR200284 Smim8 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810030N24 gene (1810030N24Rik) 10 ug
$289.00
MR200284L3 Lenti ORF clone of Smim8 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810030N24 gene (1810030N24Rik) 10 ug
$450.00
MR200284L4 Lenti ORF clone of Smim8 (mGFP-tagged) - Mouse RIKEN cDNA 1810030N24 gene (1810030N24Rik) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.