Hmgn1 (NM_008251) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG200269
Hmgn1 (tGFP-tagged) - Mouse high mobility group nucleosomal binding domain 1 (Hmgn1)
$350.00
3 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hmgn1 |
| Synonyms | HMG-14; Hmg14 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008251 |
| ORF Size | 288 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008251.3, NP_032277.3 |
| RefSeq Size | 1200 bp |
| RefSeq ORF | 291 bp |
| Locus ID | 15312 |
| UniProt ID | P18608 |
| Cytogenetics | 16 56.83 cM |
| Summary | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008251" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC207300 | Hmgn1 (untagged) - Mouse high mobility group nucleosomal binding domain 1 (Hmgn1), (10ug) | 10 ug |
$165.00
|
|
| MR200269 | Hmgn1 (Myc-DDK-tagged) - Mouse high mobility group nucleosomal binding domain 1 (Hmgn1) | 10 ug |
$289.00
|
|
| MR200269L3 | Lenti ORF clone of Hmgn1 (Myc-DDK-tagged) - Mouse high mobility group nucleosomal binding domain 1 (Hmgn1) | 10 ug |
$450.00
|
|
| MR200269L4 | Lenti ORF clone of Hmgn1 (mGFP-tagged) - Mouse high mobility group nucleosomal binding domain 1 (Hmgn1) | 10 ug |
$450.00
|
|
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Plasmid Map for MG200269
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Cloning scheme for MG200269
Circular map for MG200269
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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