OriGene Logo

Hbxip (BC028547) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG200230
Hbxip (tGFP-tagged) - Mouse hepatitis B virus x interacting protein (cDNA clone MGC:41446 IMAGE:1513442)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Hbxip or BC028547"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hbxip
Synonyms 1110003H18Rik; Hbxip; XIP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200230 representing BC028547
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGACTTTGGAGCAGCATTTGGAGGACACAATGAAGAATCCATCCATTGTTGGAGTCCTATGCA
CAGATTCACAAGGACTTAATCTGGGCTGCCGTGGTACCCTGTCGGATGAGCACGCTGGAGTCATATCTGT
TCTAGCCCAGCAGGCAGCTAGGCTAACCTCTGACCCCACCGACATCCCTGTGGTATGTTTAGAATCAGAT
AATGGGAACATTATGATCCAGAAACACGATGGCATCACAGTGGCTGTGCACAAAATGGCCTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200230 representing BC028547
Red=Cloning site Green=Tags(s)

MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPTDIPVVCLESD
NGNIMIQKHDGITVAVHKMAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC028547
ORF Size 273 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028547, AAH28547
RefSeq Size 735 bp
RefSeq ORF 275 bp
Locus ID 68576
Cytogenetics 3 F2.3
Summary As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"BC028547" in other vectors (4)
SKU Description Size Price
MC206925 Hbxip (untagged) - Mouse hepatitis B virus x interacting protein (cDNA clone MGC:41446 IMAGE:1513442), (10ug) 10 ug
$165.00
MR200230 Hbxip (Myc-DDK-tagged) - Mouse hepatitis B virus x interacting protein (cDNA clone MGC:41446 IMAGE:1513442) 10 ug
$289.00
MR200230L3 Lenti ORF clone of Hbxip (Myc-DDK-tagged) - Mouse hepatitis B virus x interacting protein (cDNA clone MGC:41446 IMAGE:1513442) 10 ug
$450.00
MR200230L4 Lenti ORF clone of Hbxip (mGFP-tagged) - Mouse hepatitis B virus x interacting protein (cDNA clone MGC:41446 IMAGE:1513442) 10 ug
$450.00

Welcome to OriGene AI!

I'm your AI-powered guide to everything OriGene.
Ask me anything:

  • Product specs
  • Ordering help
  • Technical questions
  • How to find exactly what your experiment needs

Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.

Velaro Chat Img

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.