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Srp9 (NM_012058) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200190
Srp9 (tGFP-tagged) - Mouse signal recognition particle 9 (Srp9)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Srp9
Synonyms 9kDa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200190 representing NM_012058
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCAGTTCCAGACCTGGGAGGAGTTCAGCCGGGCGGCCGAGAAGCTCTACCTGGCGGACCCCATGA
AGGTACGGGTGGTTCTCAAATACAGGCATGTTGATGGGAATTTGTGTATCAAAGTAACGGATGATCTAGT
TTGTTTGGTGTACAGAACAGACCAAGCGCAAGACGTAAAGAAGATTGAGAAATTCCACAGTCAGTTAATG
CGACTTATGGTGGCCAAGGAATCCCGCAATGTCACTATGGAAACAGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200190 representing NM_012058
Red=Cloning site Green=Tags(s)

MPQFQTWEEFSRAAEKLYLADPMKVRVVLKYRHVDGNLCIKVTDDLVCLVYRTDQAQDVKKIEKFHSQLM
RLMVAKESRNVTMETE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012058
ORF Size 258 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012058.3, NP_036188.1
RefSeq Size 1363 bp
RefSeq ORF 261 bp
Locus ID 27058
UniProt ID P49962
Cytogenetics 1 H5
Summary Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_012058" in other vectors (4)
SKU Description Size Price
MC207470 Srp9 (untagged) - Mouse signal recognition particle 9 (Srp9), (10ug) 10 ug
$165.00
MR200190 Srp9 (Myc-DDK-tagged) - Mouse signal recognition particle 9 (Srp9) 10 ug
$289.00
MR200190L3 Lenti ORF clone of Srp9 (Myc-DDK-tagged) - Mouse signal recognition particle 9 (Srp9) 10 ug
$450.00
MR200190L4 Lenti ORF clone of Srp9 (mGFP-tagged) - Mouse signal recognition particle 9 (Srp9) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.