Cdc42se2 (NM_178626) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200176
Cdc42se2 (tGFP-tagged) - Mouse CDC42 small effector 2 (Cdc42se2)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdc42se2
Synonyms 2810404F18Rik; AA408783; AA536669; SPEC2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200176 representing NM_178626
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAATTCTGGTTGTGTTTCAACTGCTGTATTGCAGAGCAGCCTCAGCCTAAGAGGCGACGACGGA
TTGACCGAAGTATGATCGGAGAGCCAACCAACTTTGTGCACACAGCTCACGTAGGATCCGGAGACCTATT
TAGTGGGATGAACTCAGTCAGCTCCATTCAGAACCAGATGCAGTCCAAGGGAGGCTATGGAGGTGGAATG
CCTGCCAACGTGCAGATGCAGCTCGTGGACACCAAGGCAGGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200176 representing NM_178626
Red=Cloning site Green=Tags(s)

MSEFWLCFNCCIAEQPQPKRRRRIDRSMIGEPTNFVHTAHVGSGDLFSGMNSVSSIQNQMQSKGGYGGGM
PANVQMQLVDTKAG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178626
ORF Size 252 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178626.3, NP_848741.1
RefSeq Size 3138 bp
RefSeq ORF 255 bp
Locus ID 72729
UniProt ID Q8BGH7
Cytogenetics 11 B1.3
Summary Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178626" in other vectors (4)
SKU Description Size Price
MC211354 Cdc42se2 (untagged) - Mouse CDC42 small effector 2 (Cdc42se2), (10ug) 10 ug
$165.00
MR200176 Cdc42se2 (Myc-DDK-tagged) - Mouse CDC42 small effector 2 (Cdc42se2) 10 ug
$289.00
MR200176L3 Lenti ORF clone of Cdc42se2 (Myc-DDK-tagged) - Mouse CDC42 small effector 2 (Cdc42se2) 10 ug
$450.00
MR200176L4 Lenti ORF clone of Cdc42se2 (mGFP-tagged) - Mouse CDC42 small effector 2 (Cdc42se2) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.