Cox7a2 (NM_009945) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200168
Cox7a2 (tGFP-tagged) - Mouse cytochrome c oxidase, subunit VIIa 2 (Cox7a2)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cox7a2
Synonyms Cox7a3; COX7AL; CoxVIIa-L
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200168 representing NM_009945
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCGGAATCTGCTGGCCCTTCGTCAGATTGCCCAGAGGACCATCAGCACCACTTCACGAAGGCATT
TTGAAAACAAGGTTCCAGAGAAACAAAAGCTGTTTCAGGAGGATAATGGGATGCCAGTTCATCTGAAAGG
CGGGGCATCTGATGCCCTCCTCTACAGAGCCACAATGGCTCTGACGCTTGGTGGCACAGCCTATGCCATC
TATCTGTTAGCTATGGCTGCATTTCCCAAGAAGCAGAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200168 representing NM_009945
Red=Cloning site Green=Tags(s)

MLRNLLALRQIAQRTISTTSRRHFENKVPEKQKLFQEDNGMPVHLKGGASDALLYRATMALTLGGTAYAI
YLLAMAAFPKKQN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009945
ORF Size 249 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009945.3, NP_034075.2
RefSeq Size 754 bp
RefSeq ORF 252 bp
Locus ID 12866
UniProt ID P48771
Cytogenetics 9 43.82 cM
Summary This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009945" in other vectors (4)
SKU Description Size Price
MC200850 Cox7a2 (untagged) - Mouse cytochrome c oxidase, subunit VIIa 2 (Cox7a2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MR200168 Cox7a2 (Myc-DDK-tagged) - Mouse cytochrome c oxidase, subunit VIIa 2 (Cox7a2), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR200168L3 Lenti ORF clone of Cox7a2 (Myc-DDK-tagged) - Mouse cytochrome c oxidase, subunit VIIa 2 (Cox7a2), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200168L4 Lenti ORF clone of Cox7a2 (mGFP-tagged) - Mouse cytochrome c oxidase, subunit VIIa 2 (Cox7a2), nuclear gene encoding mitochondrial protein 10 ug
$450.00

Plasmid Map for MG200168

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Cloning scheme for MG200168

Circular map for MG200168

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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