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Sptssa (NM_134054) Mouse Tagged ORF Clone

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SKU
MG200062
Sptssa (tGFP-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sptssa
Synonyms 1110002B05Rik; AA407909; AA409588; AU041967; Ssspta
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200062 representing NM_134054
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGGCGCGAGCATGGAAGCAGATGTCCTGGTTCTACTACCAGTACCTGCTGGTCACTGCGCTCT
ACATGCTGGAGCCCTGGGAGCGAACCGTGTTCAATTCGATGCTGGTTTCCGTGGTGGGGATGGCCCTGTA
CACTGGCTACGTCTTCATGCCCCAGCACATCATGGCTATTCTGCATTACTTTGAAATTGTACAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200062 representing NM_134054
Red=Cloning site Green=Tags(s)

MALARAWKQMSWFYYQYLLVTALYMLEPWERTVFNSMLVSVVGMALYTGYVFMPQHIMAILHYFEIVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134054
ORF Size 204 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134054.1
RefSeq Size 1363 bp
RefSeq ORF 216 bp
Locus ID 104725
UniProt ID Q8R207
Cytogenetics 12 C1
Summary Stimulates the activity of serine palmitoyltransferase (SPT). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. Plays a role in MBOAT7 location to mitochondria-associated membranes (MAMs), may me involved in fatty acid remodeling phosphatidylinositol (PI).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134054" in other vectors (6)
SKU Description Size Price
MC207810 Sptssa (untagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik), (10ug) 10 ug
$165.00
MR200062 Sptssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) 10 ug
$289.00
MR200062L1 Lenti ORF clone of Sptssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) 10 ug
$450.00
MR200062L2 Lenti ORF clone of Sptssa (mGFP-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) 10 ug
$450.00
MR200062L3 Lenti ORF clone of Sptssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) 10 ug
$450.00
MR200062L4 Lenti ORF clone of Sptssa (mGFP-tagged) - Mouse RIKEN cDNA 1110002B05 gene (1110002B05Rik) 10 ug
$450.00

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