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Tomm7 (NM_025394) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200023
Tomm7 (tGFP-tagged) - Mouse translocase of outer mitochondrial membrane 7 homolog (yeast) (Tomm7)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tomm7 or NM_025394"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tomm7
Synonyms 1110020J08Rik; AW047273; Tom7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200023 representing NM_025394
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGCTGAGCAAAGAAGCCAAACAGAGGCTGCAGCAGCTCTTCAAGGGCGGCCAGTTTGCCATCC
GCTGGGGCTTTATTCCTCTCGTGATTTACCTGGGATTTACAAGGGGTGCAGATCCTGGAATGCCTGAACC
GTCGGTTTTAAGCCTACTTTGGGGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200023 representing NM_025394
Red=Cloning site Green=Tags(s)

MVKLSKEAKQRLQQLFKGGQFAIRWGFIPLVIYLGFTRGADPGMPEPSVLSLLWG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025394
ORF Size 165 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025394.3, NP_079670.1
RefSeq Size 1126 bp
RefSeq ORF 168 bp
Locus ID 66169
UniProt ID Q9D173
Cytogenetics 5 A3
Summary Required for assembly and stability of the TOM complex (By similarity). Positive regulator of PRKN translocation to damaged mitochondria. Acts probably by stabilizing PINK1 on the outer membrane of depolarized mitochondria (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025394" in other vectors (4)
SKU Description Size Price
MC203313 Tomm7 (untagged) - Mouse translocase of outer mitochondrial membrane 7 homolog (yeast) (Tomm7), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MR200023 Tomm7 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 7 homolog (yeast) (Tomm7), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR200023L3 Lenti ORF clone of Tomm7 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 7 homolog (yeast) (Tomm7), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200023L4 Lenti ORF clone of Tomm7 (mGFP-tagged) - Mouse translocase of outer mitochondrial membrane 7 homolog (yeast) (Tomm7), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.