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Ampd2 (NM_001289719) Mouse Untagged Clone

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SKU
MC228954
Ampd2 (untagged) - Mouse adenosine monophosphate deaminase 2 (Ampd2), transcript variant 3
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $805.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ampd2
Synonyms 1200014F01Rik; AI552571; Ampd-2; m4521Dajl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC228954 representing NM_001289719
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAGAAGCTCGAAGCGGTCTGGGGGCCTCTCCGCTACAGTCTGCCCGATCCCTGCCAGGCAACG
CCCCTTGCCTCAAGCACTTCCCGCTTGACCTGCGCACGTCTATGGATGGCAAATGCAAGGAGATCGCTGA
GGAGTTGTTCAGCCGCTCACTGGCTGAGAGTGAGCTTCGTAGCGCCCCTTATGAGTTCCCAGAGGAAAGC
CCCATCGAGCAGCTAGAAGAACGGAGGCAGCGGCTGGAGCGCCAGATCAGCCAGGATGTCAAGCTGGAGC
CAGATATTCTTCTTCGAGCCAAGCAAGATTTCCTGAAGACAGACAGCGACTCAGACTTACAGCTGTACAA
GGAGCAAGGAGAGGGACAGGGTGACAGGGGTCTTTGGGAACGTGATGTGGTATTGGAACGGGAATTTCAG
CGGGTCATCATCTCTGGGGAGGAGAAGTGTGGGGTGCCATTCACAGACCTCTTAGACGCAGCCAAAAGTG
TGGTTCGGGCACTTTTCATCCGGGAGAAGTACATGGCCCTATCACTGCAGAGCTTCTGTCCCACCACCCG
CCGTTACCTGCAGCAGCTGGCTGAGAAGCCCCTGGAGACTCGAACTTATGAGCAGAGTCCTGATACCCCT
GTATCTGCTGATGCCCCAGTGCATCCCCCTGCACTGGAGCAGCACCCGTATGAGCACTGTGAGCCAAGCG
CCATGCCTGGGGACCTGGGCTTGGGTCTGCGCATGGTGCGTGGTGTGGTGCACGTCTACACCCGCAGGGA
CCCTGATGAGCACTGTCCGGAGGTGGAGCTTCCATACCCTGACCTACAGGAATTTGTAGCTGACGTCAAT
GTGCTGATGGCCCTGATCATCAATGGTCCCATAAAGTCATTCTGCTACCGCCGGCTGCAGTACCTGAGCT
CCAAATTCCAGATGCACGTTTTGCTCAATGAGATGAAGGAGCTCGCTGCTCAGAAGAAAGTGCCACACCG
GGACTTCTACAATATCCGTAAGGTGGACACACACATCCACGCCTCGTCCTGCATGAACCAGAAACATCTA
CTGCGCTTCATCAAGCGGGCCATGAAGCGGCACCTGGAGGAGATTGTGCATGTGGAACAGGGCCGCGAGC
AGACGCTGAGAGAAGTCTTCGAGAGCATGAACCTCACTGCCTACGACTTAAGTGTGGACACGCTGGATGT
GCATGCGGACAGGAATACCTTTCATCGATTTGACAAATTCAATGCCAAATACAACCCTATTGGGGAGTCT
GTTCTCCGAGAGATCTTCATTAAAACCGACAACAAGATTTCTGGGAAGTACTTTGCTCACATCATCAAGG
AGGTGATGGCAGACTTGGAGGAGAGCAAATACCAGAATGCAGAGCTCCGGCTGTCCATCTACGGGCGTTC
GAGGGATGAGTGGGACAAGCTGGCACGCTGGGCAGTGAACCACAAAGTGCACTCTCCCAATGTCCGCTGG
CTGGTGCAGGTGCCCCGCCTCTTCGATGTGTACCGCACCAAGGGCCAGCTGGCCAACTTCCAAGAGATGC
TGGAGAACATCTTTCTGCCCCTGTTTGAGGCTACTGTGCACCCTGCCAGCCACCCGGAGCTGCACCTCTT
TCTGGAGCACGTGGATGGTTTTGATAGCGTGGATGATGAGTCCAAGCCAGAGAACCACGTCTTCAACCTG
GAGAGTCCCCTCCCAGAAGCTTGGGTGGAGGAGGACAACCCTCCCTATGCCTACTACCTGTACTACACCT
TCGCTAACATGGCTATGTTGAACCATCTGCGCAGGCAGAGAGGTTTCCACACGTTCGTGCTGAGGCCGCA
CTGTGGGGAGGCCGGGCCCATCCACCACCTGGTATCAGCCTTCATGCTGGCCGAGAACATCTCCCACGGG
CTGCTCCTGCGCAAGGCCCCCGTCCTGCAGTACCTGTATTACCTGGCTCAGATCGGCATCGCCATGTCCC
CGCTCAGCAACAACAGCCTGTTCCTCAGCTACCACCGGAACCCTCTCCCTGAGTACTTGTCCCGTGGCCT
CATGGTCTCGCTGTCCACAGATGATCCCTTGCAGTTCCACTTCACCAAGGAGCCCCTGATGGAGGAGTAC
AGCATCGCCACCCAGGTGTGGAAGCTCAGCTCCTGCGATATGTGCGAGCTGGCCCGTAACAGCGTGCTCA
TGAGTGGCTTCTCTCACAAGGTGAAAAGCCACTGGCTGGGACCCAACTATACCAAGGAGGGCCCTGAGGG
CAATGATATCCGCCGTACCAACGTGCCAGACATCCGAGTGGGCTACCGCTATGAGACGCTATGCCAGGAG
CTGGCACTTATCACACAGGCCGTCCAAAGTGAGATGCTGGAGACCATCCCAGAGGAAGTGGGCATTGTCA
TGAGCCCAGGGCCTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001289719
Insert Size 2397 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001289719.1, NP_001276648.1
RefSeq Size 3608 bp
RefSeq ORF 2397 bp
Locus ID 109674
UniProt ID Q9DBT5
Cytogenetics 3 46.83 cM
Summary AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform a. Variants 2 and 3 both encode the same isoform (b).
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Documents
Resources
"NM_001289719" in other vectors (1)
SKU Description Size Price
MR231165 Ampd2 (myc-DDK-tagged) - Mouse adenosine monophosphate deaminase 2 (Ampd2), transcript variant 3 10 ug
$589.00 $732.00

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