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Tjap1 (NM_001252475) Mouse Untagged Clone

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SKU
MC228228
Tjap1 (untagged) - Mouse tight junction associated protein 1 (Tjap1), transcript variant 4
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $553.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tjap1
Synonyms 0610041D19Rik; AI415281; AW121008; Pilt; Tjp4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC228228 representing NM_001252475
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGTGCTGCCCCTGCTAAGAAACCTTACCGTAAGGCGCCTCCAGAGCACCGAGAGCTACGCCTGG
AGATTCCCGTGTCCCGGCTCGAGCAAGAGGAGTCCCTGACTGACGCAGAGCGGATGAAACTTTTGCAGCA
GGAGAATGAAGAACTTCGCAAACGCCTGGCCTCAGCTACCAGACGCACTGAGGCCTTGGAGCGGGAGCTG
GAAATAGGACAAGACTGCCTAGAACTGGAGTTAGGACAGAGCCGTGAGGAGCTGGACAAGTTTAAGGACA
AATTCCGCAGGCTACAGAACAGCTACACGGCATCCCAGCGGACCAACCAGGAACTGGAGGACAAGCTGCA
CGCACTGATCAAGAAGGCTGAGATGGACAGGAAGACGCTGGACTGGGAGATTGTGGAGCTGACCAACAAG
CTACTGGATGCCAGGAACACCATCAATAAACTGGAAGAGCTCAATGAGCGGTACCGGCTGGACTGCAACC
TGGCGGTGCAGCTTCTCAAGTGCAACAAGTCCCACTTCCGGAACCACAAGCTTGCTGACCTACCCTGTGA
GCTCCAGGACATGGTTCGAAAACATCTGCGCAGTGGGCAGGAGGTTGCCAGCCCCAGCCCCAGCCCTTCT
TCCAGCCTGTCCCCAGGGGCTGTGGTGCCTACCTCAGTCATTGCCCGGGTGTTGGAGAAACCTGAGTCTC
TCTTGCTCAATTCAGCTCAGTCTGGCAGTGCTGGGCGCCCCTTGGCTGAGGATGTATTCGTGCATGTGGA
CATGAGTGGGGGTGACCCAGCCAGCCCCCCTGCCCCGGGCAGCCCCAATGGGGAGTGTTGCTCAGTGAGT
ACTGCAGGGGGCTCCCCGGAGGAGGAGCTGCCCCTGCCAGCCTTCGACAAGTTGAGTCCCTACCCTACCC
CGTCTCCACCACACCCATTATACCCTGGTAGGAAGGTGATAGAATTCTCTGAGGACAAGATCCGAATTCC
TCGGAACAGCCCCCTCCCCAACTGTACCTATGCCACCCGGCAGGCCATCTCCTTGAGCCTGGTGGAGGAC
GGCAGTGAGCGGGCCCACCGCAGCTCAGTGCCTAGCAGTCCTGCCTCTGCCCAAGGGTCGCCCCATCACC
AGCCTAGTCCAGCTCCCTCAGCACTGAGTGCCCCAGCTAGTTCGGCCAGCTCGGAGGAGGACCTGCTGGC
CAGCTGGCAGCGTGCATTTGTGGACCGCACCCCGCCCCCTGCTGCCGTGGTCCAGCGCACGGCCTTTGGA
CGAGACTCACTGCCCGAGCTACAATTGCATTTCTCCCCGGGCCACTCCACAGCCCCACCGCCTTCCCCGC
ACCGTGAGCGGGGACTTGTGCTGCCAGCAGAACCTGACTCTGGCTTTCCTCAAGACGAGGAAGAGGAAAT
GCTGAACCTGCCTGTCAGCCCCGAGGAAGAGCGTCAGAGCCTGCTGCCTGATAAAGAGGGCACCGAGGAG
GCCTCTGGCCCTTCTCATGTTGATGGCAGGGCCTGGCCTCTCCCCAGTCCCAGCCGCCCCCAGCGCAGCC
CCAAGAGAATGGGAGTACACCATCTCCACCGCAAGGACAGCCTGACGCAGGCACAGGAGCAGGGCACTGT
GCTCAGCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001252475
Insert Size 1620 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001252475.1, NP_001239404.1
RefSeq Size 2368 bp
RefSeq ORF 1620 bp
Locus ID 74094
UniProt ID Q9DCD5
Cytogenetics 17 C
Summary Plays a role in regulating the structure of the Golgi apparatus.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_001252475" in other vectors (1)
SKU Description Size Price
MR230439 Tjap1 (myc-DDK-tagged) - Mouse tight junction associated protein 1 (Tjap1), transcript variant 4 10 ug
$289.00 $502.00

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