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Ift172 (NM_026298) Mouse Untagged Clone

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SKU
MC224902
Ift172 (untagged) - Mouse intraflagellar transport 172 homolog (Chlamydomonas) (Ift172), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $2,352.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ift172
Synonyms 4930553F24Rik; avc1; Slb; wim
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224902 representing NM_026298
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGAGCTGCGAAGGTGACATGCATGGCCT
GGTCCCAGAACAATGCTAAATTTGCTGTCTGCACAGTGGACCGAGTGGTACTTCTGTATGATGAACATGG
GGAGCGGAGAGACAAGTTCTCCACCAAACCAGCTGACATGAAGTACGGCAGGAAAAGCTACATGGTGAAA
GGCATGGCTTTCTCTCCCGATTCCACAAAAATTGCCATAGGACAGACTGACAACATCATCTATGTCTACA
AGATTGGAGAAGATTGGGGAGACAAGAAGGTCATCTGCAACAAGTTCATCCAGACGAGTGCTGTGACTTG
TCTGCAGTGGCCTGCGGAATACGTCATTGTCTTTGGACTGGCAGAAGGCAAGGTTCGCTTAGCAAACACT
AAAACTAACAAGTCATCTACTATCTATGGGACAGAGTCTTACGTGGTAGCACTGACAACCAACTGCTCTG
GGAAGGGGATCCTCTCGGGTCACGCAGACGGCACCATCGTCAGGTACTTCTTCGATGATGAAGGCTCTGG
AGAGTCACAGGGAAAGTTGGTTAACCATCCATGCCCACCCTATGCCTTGGCCTGGGCCACCAACAGCATT
GTGGCCGCTGGCTGTGACCGGAGGATTGTGGCCTATGGAAAGGAAGGCCATGTGCTGCAGACTTTTGACT
ATAGCCGAGACCCTCAGGAGCGGGAGTTCACCACAGCTGCTGCAAGTCCTGGAGGCCAGTCTGTTGTGCT
GGGAAGTTATGACAGACTTCGGGTGTTCAACTGGAGTCCTCGAAGAAGCATCTGGGAAGAGGCGAAACCC
AAGGAGATCGCTAACTTATACACCGTCACCGCCCTGGCCTGGAAGCGGGATGGCTCACGACTCTGTGCGG
GAACACTCTGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAAGGAGCATTTACAAGAACAAGTTCGA
GCTGACGTATGTGGGCCCTAGCCAGGTGATTGTGAAGAACCTGTCTTCAGGGACCAGGGTGGTGCTCAAG
TCACACTATGGCTATGAAGTAGAAGAGGTGAAAATCCTGGGAAAGGAACGTTACTTGGTGGCTCACACAT
CAGACACACTGCTGCTGGGAGACCTGAACACGAACCGCCTCAGCGAGATAGCCTGGCAGGGATCTGGGGG
CAATGAGAAATATTTCTTTGAAAATGAGAATGTGTGCATGATCTTCAATGCTGGAGAGCTGACCCTGGTG
GAATATGGGAGTAACGACTCCTTGGGTTCTGTGCGCACCGAATTCATGAACCCTCACCTCATCAGCGTCC
GTATCAATGAGCGCTGCCAGCGAGGAATGGAAGATAATAAGAAATTGGCTTATCTTGTTGATATTAAGAC
AATTGCTATAGTGGATCTGATTGGTGGCTACAACATTGGTACTATCAGTCACGAAAGCCGGGTGGATTGG
CTAGAACTTAACGAGACTGGACACAAGCTTCTCTTTAGAGACCGGAAACTTCGGCTGCATCTATATGATA
TTGAAAGCTGCTCTAAGACAATGATCCTCAACTTCTGCTCCTACGTGCAGTGGGTTCCGGGGAGTGATGT
GCTGGTAGCTCAGAACCGAAACAGCTTATGTGTATGGTACAATATTGAGGCCCCTGAGAGAGTCACCATG
TCCTCTATCAGGGGTGATGTTGTCGGTCTGGAGCGAGGTGGTGGGAAGACAGAGGTCATGGTGACAGAAG
GCGTGACTACTGTGGCCTACACCCTGGACGAGGGCCTGATTGAGTTTGGAACAGCCATCGATGATGGCAA
CTACACCCGGGCTACAGCCTTCTTGGAGACCCTGGAGATGACTCCCGAAACAGAGGCAATGTGGAAAACC
TTGAGCAAACTGGCCCTGGAGGCAAGGCAGCTTCACACTGCTGAGAGGTGCTTCTCTGCTTTAGGTCATG
TGGCCAAAGCTCGGTTCCTGCATGAGACCAATGAGATTGCAGATCAGGTGTCCAGGGAATATGGTGGAGA
AGGAACGGACTTCTATCAGGTCCGAGCTCGTCTCGCCATGCTGGAGAAGAACTACAAACTTGCTGAGATG
ATCTTCTTGGAACAGAATGCTGTTGAGGAGGCCATGGACATGTACCAAGAGTTGCACCGCTGGGAGGAAT
GCATTGCTGTGGCTGAGGCCAAGGGACACCCAGCCCTGGAGAAGCTGCGCAGAGACTACTATCAATGGTT
AATGGACACACAGCAAGAGGAACGAGCAGGTGAACTGCAGGAGAGCCAAGGGGATGGGCTGGCAGCTATC
AGCCTCTACCTCAAAGCTGGGCTCCCTGCCAAAGCTGCGCGGCTGGTGCTGACCCGAGAGGAGCTGCTGG
CCAACACAGAACTAGTAGAACATATCACCACAGCCCTCATCAAGGGAGAGCTCTATGAAAGGGCAGGTGA
TCTCTTTGAGAAGATTCGAAATCCACAGCGGGCCCTTGAGTGTTACTGTAAAGGCAATGCATTCATGAAA
GCTGTTGAGCTGGCTCGACTGGCCTTCCCAGTGGAGGTGGTGAGGCTAGAGGAGGCCTGGGGGGACCACT
TGGTACAGCAAAAGCAGCTGGATGCGGCCATCAACCACTATATTGAAGCCAGGTGCTCCATCAAAGCAAT
TGAGGCTGCCCTGGGTGCCCGCCAGTGGAAGAAGGCAATTTATATATTAGATCTCCAGGATCGCAACACT
GCATCTAAATACTATCCTCGTGTGGCTCAACACTATGCGTCTCTGCAGGAGTATGAGATTGCTGAGGAGC
TCTACACCAAAGGAGACCGGACCAAAGACGCCATAGACATGTACACCCAGGCCGGCCGCTGGGAGCAAGC
CCACAAGCTGGCCATGAAATGCATGCGACCCGAGGACGTGTCCGTGCTTTACATCACACAAGCCCAGGAG
ATGGAGAAGCAGGGCAAGTACCGAGAGGCTGAGAGGCTGTACGTGACGGTGGAGGAGCCTGATCTCGCCA
TCACCATGTTCAAAAAGCACAAGTTGTATGACGACATGATTCGCCTGGTGGGGAAGCACCATCCAGACCT
CCTCAGCGACACACACCTTCACCTGGGCAAGGAGCTGGAGGCAGAAGGCCGACTGCAGGAGGCTGAGTAT
CACTACCTTGAGGCCCAGGAATGGAAGGCAACTGTGAACATGTACCGCTCCAGCGGGCTTTGGGAGGAGG
CCTACCGGGTGGCCAAAGCCCATGGAGGAGCCAATGCCCACAAACACGTGGCCTATCTGTGGGCCAAAAG
CCTGGGAGGAGAGGCTGCAGTTAGACTACTTAACAAGCTGGGACTTCTGGAGGCCACCATTGACCATGCT
GCTGACAACTGCTCCTTTGAGTTTGCCTTTGAACTCTCTCGGCTAGCCTTCAAGCACAAAGCCCCAGAGA
TTCATCTCAAATACGCCATGTACCTGGAAGATGAGGGCAAATTTGAAGAGGCTGAAGCTGAATTCATCCG
AGCTGGTAAACCCAAAGAGGCGGTCCTCATGTTTGTCCACAACCAGGACTGGGAGGCAGCTCAGCGCGTG
GCTGAGGCCCACGATCCTGACAGCGTTGCTGAGGTACTTGTAGGTCAAGCCCGTGGGGCCTTGGAGGAGA
AGGACTTTCAGAAGGCAGAAGGGTTACTACTTCGGGCCCAGAGACCAGGTCTGGCTCTCAATTATTATAA
GGAGGCTGGACTCTGGAGCGACGCTCTGAGAATCTGCAAGGACTATGTGCCTGGCCAGCTAGAGGCCCTA
CAGGAAGAGTATGAGCGAGAAGCTACCAAGAAGGGAGGCAGAGGGGTGGAGGGACTTGTAGAGCAAGCAC
GGCAGTGGGAACAGGCTGGAGAGTATAGCCGTGCAGTGGACTGCTACCTCAAAGTTCGCGACTCAGGAAG
CAGTGGCCTGATGGAGAAGTGCTGGATGAAGGCGGCTGAACTGTCCATCAAGTTCCTGCCTCCCCAGCGC
AGTCTGGAAGTCGTCCGGGTTGTAGGGCCCCAGCTGATCGGGATTGGAAAGCACAGTGCTGCTGCAGAAC
TCTATCTGAACCTGGACCTTGTCAAAGAAGCAATCGATGCTTTCATTGAGGGGGAAGAGTGGAACAAGGC
CAAGCGCGTGGCCAAAGAGCTAGATCCCCGGTATGAGGACTATGTGGATCAGCACTATAAGGAGTTCCTC
AAGAACCAGGGCAAAGTGGATTCGCTGGTGGGTGTGGACGTAGTAGCTGCCTTGGACCTGTATGTGGAGC
AGGGCCAGTGGGATAAATGCATTGAAACAGCTACCAAGCAGAACTACAAGATTCTGCACAAGTATGTGGC
TTTGTATGCAACTCATCTGATCCGAGAAGGTGGCTATGCCCAGGCACTGGCCCTCTATGTGCAACACGGA
GCCCCTGCAAACCCACAGAATTTCAACATCTACAAAAGGATCTTCACTGACATGGTTAGCTCTCCTGGGA
CCAACAATGCTGAGGCCTACCATAGCTGGGCTGATCTTCGGGATGTCCTCTTCAACCTGTGTGAAAACCT
GGTGAAGTCCAGTGAGGCAAACTCTGCAGCGCATGAGGAGTTTGAGATGATGCTGCTGATCTCTCATTAC
TACGCTACGCGATCTGCAGCCCAGAGTATCAAACAGCTGGAAACCGTAGCTGCCAGGCTTTCTGTTTCGC
TCTTGCGCCACACCCAGTTACTCCCTGCAGACAAGGCCTTTTATGAAGCGGGCACTGCTGCCAAGGAAGT
TGGCTGGGAGAACATGGCTTTCATTTTCCTCAATCGGTTCTTGGATCTGACTGATGCAATCGAAGAAGGG
ACCCTGGATGCCCTGGACCATTCAGATTTTCAGGATACTGACATTCCCTTTGAGGTGCCGCTCCCAGCCA
AGCAGCACGTCCCGGAGGCCCAGAGAGAAGAGGTCCGAGACTGGGTGCTCACAGTCTCGATGGACCAGAG
ACTGGAGCAGGTTCTGCCCCGGGATGAGCGAGGTGTCTATGAGGCTTCCCTAGTTGCCGCGAGCACTGGA
GTTCGGGCACTGCCCTGCCTCATCACAGGTTACCCCATTTTGAGGAACAAAATTGAATTTAAGCGGCCTG
GGAAGGCTGCTAACAAAGACAACTGGAACAAGTTTCTTATGGCCATCAAGACCTCTCACAGCCCAGTGTG
CCAGGACGTGCTCAAGTTCATCAGCCAGTGGTGCGGTGGTCTCCCTAGCACCAGCTTTTCCTTTCAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_026298
Insert Size 5250 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026298.5, NP_080574.5
RefSeq Size 5440 bp
RefSeq ORF 5250 bp
Locus ID 67661
UniProt ID Q6VH22
Cytogenetics 5 B1
Summary Required for the maintenance and formation of cilia. Plays an indirect role in hedgehog (Hh) signaling, cilia being required for all activity of the hedgehog pathway.UniProtKB/Swiss-Prot Function
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"NM_026298" in other vectors (2)
SKU Description Size Price
MG212076 Ift172 (tGFP-tagged) - Mouse intraflagellar transport 172 homolog (Chlamydomonas) (Ift172) 10 ug
$2,404.00
MR212076 Ift172 (Myc-DDK-tagged) - Mouse intraflagellar transport 172 homolog (Chlamydomonas) (Ift172) 10 ug
$2,204.00

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