Unc13b (NM_021468) Mouse Untagged Clone

SKU MC224734

Unc13b (untagged) - Mouse unc-13 homolog B (C. elegans) (Unc13b), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Unc13b
• Synonyms: Munc13-1; Munc13-2; Unc13a; Unc13h1; Unc13h2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224734 representing NM_021468; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGCTGCTCTGTGTGCGTGTTAAAAGAGCCAAATTCCAGGGTTCACCAGATAAATTTAACACATATG; TGACCCTGAAAGTCCAGAACGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAGCA; GGATTTCATGTTTGAGATCAGTCGTCTGGACCTGGGCCTAAGTGTGGAGGTGTGGAACAAGGGACTGATA; TGGGACACAATGGTGGGCACCGTGTGGATTGCACTGAAGACAATCCGTCAGTCGGATGAGGAAGGGCCCG; GGGAGTGGTCTACACTGGAGGCAGAGACGTTAATGAAAGATGATGAGATCTGTGGAACTAAAAATCCAAC; TCCTCATAAAATCTTGCTTGATACAAGATTTGAGCTGCCTTTTGACATCCCAGAGGAGGAAGCCAGATAT; TGGACCTACAAATTGGAGCAAATCAATGCCTTGGCAGATGATAATGAGTATTCTAGTCAAGAAGAAAGCC; AGAGGAAGCCATTACCCACTGCTGCCGCCCAGTGTTGTCACTGGACCTATTTGGGCTGGGGAGAGCATCA; GACCTTTGAAGACCCTGATAGTGCCGTTGATGACCGAGACAGTGACTACCGCAGTGAGACCAGCAATAGC; GCCCCACCTCCTTACCACACGACTACCCAGCCCAACGCTTCTGTACACCAGTTCCCTGTTCCTGTGCGGC; TGCCACAGCAGCTCTTTCTTCAAGGCAGTTCCCATGACTCTTGCAATGACTCTATGCAGAGTTACGACCT; TGATTATCCAGAGCGTCGGGCCCTCAGCCCTACCAGCAGTAGTAGGTATGGCTCCTCCTGTAATGTGAGT; CAAGGAAGCTCTCTGCTGAGTGAGCTGGACCAGTATCATGAGCAAGATGATGATGGTCGGGAGAGGGACT; CCATTCATTCTAGTCATAGCTATGGCAGCCTCTCCAAAGATGGCCAGGCTGGTTTGGGAGAACAAGAGAA; AGCCTTGGAAGTGACATGTGAATCAGAAAAAGAGAAAACAGGTGAATCCAAGGAGATGAGAGATGATGCT; ACAATCCACCCTCCTTCAGATCTGGTGCTGCATAAGGACCATGTCCTAGGACCCCAGGAGAGTCTTCCTG; AGGAGACCGCATCATCGCCATTTACCCAGGCCAGAGCACACTGGTTCCGAGCGGTTACCAAGGTCCGACT; CCAACTGCAGGAGATTTCAGATGATGGTGACCCCTCCTTGCCTCAGTGGCTCCCAGAAGGGCCAGCTGGA; GGGCTCTATGGCATTGACAGCATGCCAGATCTACGAAGGAAGAAGCCACTGCCACTTGTCAGTGATCTGT; CACTGGTCCAGTCACGGAAAGCTGGGATTACATCTGCTATGGCTACACGTACCTCTCTCAAGGATGAAGA; ACTGAAATCTCATGTGTATAAGAAAACTCTGCAGGCCTTAATCTACCCCATCTCGTGTACCACGCCCCAC; AACTTTGAGGTCTGGTCTGCCACTACACCTACCTATTGCTATGAGTGTGAAGGCTTGCTCTGGGGCCTTG; CCCGGCAAGGCATGCGCTGCAGCGAGTGTGGAGTCAAGTGCCATGAGAAGTGCCAGGACCTGCTCAATGC; TGACTGCCTACAGCGGGCTGCTGAAAAGAGCTCTAAGCATGGAGCTGAGGACCGAACTCAGAACATTATC; ATGGCCATGAAGGACCGTATGAAGATCCGAGAACGGAATAAACCAGAGATCTTTGAGGTTATCCGGGATG; TGTTCACAGTGAGCAAAGTTGCGCATGTGCAGCAGATGAAAACAGTAAAGCAGAGTGTCCTGGATGGCAC; CTCCAAGTGGTCAGCCAAAATTACCATCACGGTGGTTTGTGCCCAGGGCCTACAAGCCAAAGACAAGACA; GGATCCAGTGACCCTTATGTGACTGTGCAAGTTGGGAAAACCAAGAAGCGCACCAAGACCATTTTTGGAA; ATTTGAATCCTGTTTGGGAAGAGAAGTTCCATTTTGAGTGCCACAACTCTTCTGATCGAATTAAGGTACG; CGTGTGGGATGAGGATGATGACATCAAGTCCAGAGTAAAGCAGCGGCTAAAGCGAGAGTCAGATGATTTC; CTTGGCCAAACTATCATTGAAGTTCGAACGCTGAGTGGAGAGATGGATGTCTGGTACAATCTGGAGAAGA; GAACAGACAAGTCAGCTGTCTCAGGGGCTATCCGACTTCAAATCAGTGTGGAGATCAAGGGGGAGGAGAA; GGTAGCCCCATACCATGTACAATATACATGTCTCCATGAGAACCTTTTCCATTACCTCACAGACATTCAG; GGCAGTGGAGGAGTCTGGATCCCAGAGGCTCGGGGGGACGATGCATGGAAGGTGTACTTTGATGAGACAG; CCCAAGAAATTGTGGATGAATTCGCTATGCGCTATGGCATCGAGTCCATTTATCAGGCCATGACGCACTT; TGCATGTTTGTCATCCAAGTACATGTGTCCTGGTGTACCAGCCGTGATGAGCACCTTACTGGCCAACATC; AATGCCTATTATGCTCACACAACTGCCTCCACTAACGTCTCTGCATCTGATCGTTTTGCAGCCTCCAACT; TTGGTAAAGAAAGATTTGTAAAACTGCTGGACCAGCTACACAACTCACTGAGGATAGACCTCTCTACATA; CAGGAATAACTTTCCTGCTGGGAGCCCTGAGCGGCTTCAGGATTTAAAGTCTACAGTGGATTTGCTGACC; AGCATTACTTTCTTCAGGATGAAGGTGCAAGAACTTCAGAGCCCTCCAAGAGCCAGCCAGGTGGTAAAGG; ACTGTGTGAAAGCCTGCTTGAACTCTACATATGAATACATCTTCAACAACTGCCATGACCTCTACAGTCA; CCAGTATCAGCTGCAGGAACAACCTCTAGAGGAACCAGGGCCCAGCATTCGGAACTTGGATTTCTGGCCC; AAACTTATCACACTCATTGTTTCGATCATAGAGGAGGATAAGAATTCCTACACACCTGTTCTGAGCCAGT; TTCCTCAGGAGTTGAACGTAGGAAAAGTCAGTGCAGAAGTGATGTGGCATCTGTTTGCTCAAGACATGAA; ATATGCACTGGAGGAACACGAGAAAGACCGGCTGTGTAAGAGTGCTGACTACATGAACCTGCACTTCAAG; GTGAAGTGGCTCCACAATGAATATGTGCGCGATCTGCCTGCCCTCCAGGGGCAAGTGCCTGAGTACCCAG; CGTGGTTTGAGCAGTTTGTGCTACAGTGGCTAGATGAGAATGAAGATGTGTCACTGGAATTCCTTCGTGG; GGCCTTGGAACGAGATAAGAAGGATGGGTTCCAGCAGACATCAGAGCATGCCTTGTTTTCCTGTTCTGTG; GTGGATGTCTTTACACAACTCAACCAGAGCTTTGAGATCATCCGGAAGTTGGAATGTCCAGACCCCAACA; TCCTTGCCCACTACATGAGAAGGTTTGCTAAGACCATTGGGAAAGTGCTGATGCAGTATGCAGACATCTT; ATCAAAGAACTTCCCAGCTTACTGCACAAAGGAGAGACTGCCCTGTATCCTGATGAACAACATGCAGCAA; CTGAGGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAAGAGCTGGACTCCGAAGCTGCAGACA; GTCTGAAGGAGCTGCAGGTGAAACTGAATACAGTTCTGGATGAGCTTAGCATGGTGTTTGGAAACAGCTT; CCAGGTGCGGATTGATGAATGTGTTCGACAAATGGCTGACATCCTGGGCCAAGTTCGGGGCACAGGGAAT; GCATCCCCTAACGCCAGGGCCTCGGTGGCTCAGGATGCAGATAGTGTGCTGCGACCTCTCATGGACTTCC; TGGATGGCAACCTCACGCTGTTTGCCACTGTGTGTGAGAAGACAGTTCTGAAGCGGGTCCTGAAGGAGCT; CTGGCGGGTGGTGATGAACACCATGGAGCGGGTTATTGTCCTGCCCCCACTCACTGACCAGACGGGCACC; CAGCTGATCTTAACTGCTGCCAAGGAGCTGAGCCAGCTTTCCAAACTCAAGGACCACATGGTACGGGAGG; AAACACGGAATCTCACTCCAAAGCAGTGTGCTGTCCTCGACTTGGCCTTGGACACCATCAAGCAATACTT; CCATGCAGGAGGCAATGGTCTGAAGAAAACCTTCCTGGAAAAGAGCCCAGACCTGCAGTCTCTGCGTTAT; GCCCTCTCTCTGTATACACAGACTACAGACACTCTCATCAAGACATTTGTGCGCTCACAGACTGCCCAGG; GGGCTGGTGTGGATGACCCCGTGGGAGAAGTCTCTATTCAGGTGGACTTATTTACACATCCTGGCACTGG; GGAGCATAAGGTCACAGTGAAAGTGGTAGCAGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGG; CCTTTTGTGGAAGTGACCATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTCACAACCAAGTCTA; AAAGTAACAACTGGACTCCCAAGTACAACGAGACGTTTCACTTCCTCCTGGGAAATGAAGAGGGTCCAGA; GGCCTATGAATTGCAGATCTGTGTGAAGGATTACTGCTTTGCCCGGGAGGATCGTGTGATAGGACTGGCA; GTGATGCCCCTGAGGGATGTGGCAGCTAAGGGCAGCTGTGCCTGCTGGTGCCCACTGGGCCGGAAGATCC; ATATGGATGAGACAGGCATGACCATTCTCCGGATTCTGTCTCAGAGGAGCAATGACGAGGTAGCCCGAGA; GTTCGTGAAGCTCAAATCAGAGTCTCGATCCACAGAGGAAGGGAGCTGA; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_021468
• Insert Size: 4809 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021468.3, NP_067443.2
• RefSeq Size: 6426 bp
• RefSeq ORF: 4809 bp
• Locus ID: 22249
• UniProt ID: Q9Z1N9
• Cytogenetics: 4 A5
• Summary: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP) (By similarity). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).