Top2a (NM_011623) Mouse Untagged Clone

SKU MC224642

Top2a (untagged) - Mouse topoisomerase (DNA) II alpha (Top2a), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Top2a
• Synonyms: Top-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224642 representing NM_011623; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGTTGTCACCGCTGCAGCCTGTAAATGAAAATATGCTAATGAACAAAAAGAAGAATGAAGATGGCA; AGAAAAGATTGTCTATTGAAAGAATCTATCAGAAAAAAACACAACTGGAACATATACTGCTCCGCCCAGA; TACCTACATTGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGCATTAAC; TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAGATTCTAGTTAATGCTGCTGATA; ACAAACAAAGGGACCCAAAAATGTCTTGTATTAGAGTCACAATTGATCCAGAAAATAATGTAATTAGCAT; CTGGAATAATGGGAAAGGAATCCCTGTTGTTGAACACAAAGTTGAGAAAATATATGTCCCAGCTCTCATA; TTTGGACAACTCCTGACCTCCAGTAACTATGACGACGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCT; ATGGAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGAGAGTACAAGAA; AATGTTCAAACAGACGTGGATGGATAACATGGGGAGAGCTGGTGACATGGAACTCAAGCCCTTTAGTGGA; GAAGATTATACATGTATCACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATA; TTGTTGCACTGATGGTCAGAAGAGCATATGATATCGCTGGCTCCACTAAAGATGTCAAAGTCTTTCTTAA; TGGAAATAGTCTGCCAGTGAAAGGATTCCGCAGTTACGTGGATTTGTATTTGAAGGATAAGGTAGATGAA; ACTGGGAATTCACTGAAGGTGATACATGAACAGGTCAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCG; AGAGAGGCTTCCAGCAGATTAGCTTCGTCAACAGCATTGCGACTTCTAAGGGCGGCAGACATGTCGATTA; TGTAGCTGATCAGATTGTGAGCAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGCGGGGTTGCAGTG; AAGGCTCATCAGGTGAAAAATCACATGTGGATTTTTGTAAATGCCTTAATTGAAAACCCAACCTTTGACT; CTCAAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACATGTCAATTAAGTGAAAAGTT; CATCAAAGCTGCAATTGGTTGTGGTATTGTGGAAAGCATACTAAACTGGGTGAAGTTCAAGGCTCAAATC; CAACTGAACAAGAAGTGTTCAGCTGTAAAACATACCAAAATCAAGGGAATCCCCAAACTTGATGATGCCA; ACGATGCAGGGAGCCGAAACTCTACTGAATGCACACTTATCCTGACTGAGGGAGACTCAGCAAAAACTCT; AGCCGTTTCAGGCCTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTTAGAGGAAAGATACTC; AATGTGCGAGAAGCCTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGG; GTCTCCAGTACAAGAAAAACTATGAAGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGAT; TATGACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGG; CCATCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCCATTGTAAAGGTGTCCAAAAATAAGC; AAGAAATAGCATTCTATAGTCTTCCCGAGTTTGAAGAATGGAAAAGTTCGACTCCGAATCATAAAAAGTG; GAAAGTCAAATACTACAAAGGTTTGGGCACCAGCACATCAAAGGAAGCTAAGGAATATTTTGCAGATATG; AAACGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGATGATGCTGCGATCAGCCTGGCCTTTAGTA; AAAAACAAGTTGATGATCGAAAGGAATGGTTAACTAATTTTATGGAAGACAGAAGACAGCGGAAGTTACT; TGGCCTTCCTGAGGATTATTTGTATGGACAAAGCACTTCGTACCTGACCTATAATGACTTCATAAACAAG; GAACTCATCCTCTTCTCCAACTCTGATAATGAAAGGTCTATCCCATCCATGGTAGACGGTTTGAAACCAG; GTCAGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAAGTGAAGGTTGCCCAGTTAGC; TGGGTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCACTGATGATGACCATTATCAATTTG; GCGCAGAATTTTGTGGGTAGCAATAATTTGAACCTTTTACAGCCCATTGGTCAGTTTGGAACCAGGCTGC; ATGGTGGCAAGGACTCAGCTAGTCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTGTT; TCCTCCAAAAGATGATCACACATTACGGTTTCTATATGATGACAACCAACGTGTTGAGCCTGAATGGTAC; ATTCCTATAATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCC; CCAACTTTGATGTGCGTGAAGTTGTGAATAATATCAGACGGCTTCTGGATGGAGAGGAGCCCCTACCCAT; GCTCCCAAGTTATAAGAATTTCAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGA; GAAGTAGCTATTCTGGATTCTACAACCATTGAAATCTCAGAGCTTCCCATCCGAACGTGGACTCAGACAT; ATAAAGAACAGGTTCTAGAACCCATGTTGAATGGAACTGAGAAGACACCCTCTCTAATAACAGACTATAG; GGAATACCATACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAACTGGCAGAGGCAGAG; AGAGTCGGCCTACACAAAGTCTTCAAACTCCAGAGTAGTCTCACTTGCAACTCTATGGTGCTTTTTGACC; ATGTAGGTTGCTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAA; ATATTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAA; GCTCGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAAAAAGAATTAATTA; AAGTTCTGATTCAGAGAGGCTACGACTCTGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTTCC; AGATGAAGAAGAAAATGAAGAAAGTGACACTGAAACCAGCACCAGTGACTCTGCAGCCGAGGCTGGGCCG; ACCTTCAACTACCTTCTCGACATGCCCCTGTGGTATCTGACCAAGGAGAAGAAGGATGAGCTGTGCAAAC; AAAGAAACGAGAAGGAACAAGAGCTCAACACATTAAAGCAAAAGAGTCCATCAGATCTGTGGAAGGAAGA; TCTGGCTGTTTTTATTGAAGAACTGGAGGTTGTCGAGGCCAAGGAAAAGCAGGATGAACAAGTGGGCCTT; CCTGGAAAGGCGGGGAAAGCAAAGGGGAAGAAAGCACAGATGTGTGCTGACGTCTTGCCTTCTCCTCGGG; GCAAAAGAGTCATCCCCCAAGTGACTGTGGAGATGAAAGCTGAGGCAGAGAAGAAAATTAGGAAGAAAAT; TAAGAGTGAAAACGTTGAGGGTACCCCTGCTGAGGATGGTGCGGAACCAGGAAGCCTCCGGCAACGAATA; GAGAAGAAGCAGAAGAAAGAGCCAGGTGCAAAGAAGCAGACTACATTGCCGTTTAAGCCTGTCAAAAAAG; GGAGGAAGAAAAACCCCTGGTCTGACTCTGAGTCAGACGTGAGCAGTAATGAAAGTAACGTTGATGTCCC; TCCTCGACAAAAAGAGCAAAGAAGTGCTGCAGCAAAAGCCAAATTCACAGTGGATTTAGACTCGGATGAA; GATTTCTCAGGTTTGGATGAGAAGGATGAGGATGAAGATTTCCTCCCATTAGATGCTACTCCACCTAAGG; CCAAAATCCCCCCAAAAAATACTAAAAAAGCACTGAAGACCCAGGGAAGCTCCATGTCGGTAGTTGATCT; TGAAAGTGATGTTAAGGACAGTGTGCCAGCTTCTCCAGGCGTTCCTGCTGCTGACTTCCCAGCGGAAACT; GAACAGTCAAAGCCATCCAAAAAGACCGTGGGAGTGAAGAAGACAGCAACCAAAAGCCAGTCTTCAGTCT; CCACTGCTGGTACCAAAAAGAGAGCTGCGCCAAAGGGAACCAAATCAGATTCAGCCTTGAGTGCTCGTGT; CTCGGAAAAACCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAAAGGAAGCCATCTTCTTCTGATAGCTCT; GACTCTGATTTTGAGAGAGCAATTTCTAAAGGTGCCACGAGCAAGAAAGCAAAGGGAGAGGAACAGGACT; TCCCTGTGGACTTGGAAGACACGATAGCTCCTCGAGCCAAATCTGACCGGGCGAGGAAGCCAATTAAGTA; CCTGGAGGAGTCCGATGACGATGACGACCTCTTCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011623
• Insert Size: 4587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_011623.2, NP_035753.2
• RefSeq Size: 5217 bp
• RefSeq ORF: 4587 bp
• Locus ID: 21973
• UniProt ID: Q01320
• Cytogenetics: 11 62.91 cM
• Summary: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (PubMed:24321095).[UniProtKB/Swiss-Prot Function]