Dnmt1 (BC053047) Mouse Untagged Clone
SKU
MC224567
Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259), (10ug)
$1,532.00
4 Weeks*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Dnmt1 |
| Synonyms | Dnmt, MTase, Dnmt1o |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | BC053047 |
| Insert Size | 4884 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC053047, AAH53047 |
| RefSeq Size | 5249 bp |
| RefSeq ORF | 4883 bp |
| Locus ID | 13433 |
| Cytogenetics | 9 7.66 cM |
| Summary | This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC053047" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG212057 | Dnmt1 (tGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) | 10 ug |
$789.00
$1,592.00
|
|
| MR212057 | Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) | 10 ug |
$589.00
$1,392.00
|
|
| MR212057L3 | Lenti ORF clone of Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) | 10 ug |
$1,692.00
|
|
| MR212057L4 | Lenti ORF clone of Dnmt1 (mGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259) | 10 ug |
$1,692.00
|
|
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.