Cpsf1 (NM_053193) Mouse Untagged Clone

SKU MC224463

Cpsf1 (untagged) - Mouse cleavage and polyadenylation specific factor 1 (Cpsf1), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Cpsf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224463 representing NM_053193; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTACGCGGTGTACAAGCAGGCGCACCCGCCCACCGGGCTGGAGTTTACCATGTACTGCAACTTCTTCA; ACAACAGTGAGCGCAACCTCGTGGTGGCCGGCACCTCGCAGCTCTATGTGTACCGCCTGAACCGCGACGC; CGAGGCTCTGACCAAGAATGATGGGAGCACAGAGGGGAAGGCCCACCGAGAGAAACTAGAGCTGGTAGCC; TCCTTCTCTTTTTTTGGCAATGTCATGTCCATGGCCAGTGTGCAGCTTGCAGGCGCCAAACGTGATGCCT; TGCTTCTCAGTTTCAAAGACGCTAAGCTGTCTGTGGTGGAGTATGACCCAGGCACTCATGACCTGAAGAC; CCTGTCTCTACACTACTTTGAGGAGCCTGAACTTCGGGATGGGTTTGTGCAGAACGTGCATACGCCTCGT; GTGCGGGTGGACCCTGACGGGCGCTGTGCAGCAATGCTCATCTATGGCACGAGGCTGGTAGTTCTGCCTT; TCCGCAGAGAGAGCCTGGCTGAGGAACATGAGGGGCTCATGGGCGAAGGGCAGAGGTCTAGCTTCTTGCC; CAGCTACATCATTGATGTACGGGCTCTGGATGAGAAACTACTCAACATCATTGACCTGCAGTTCCTGCAT; GGCTACTATGAGCCCACCCTGCTTATCCTGTTTGAGCCCAACCAGACTTGGCCAGGGCGGGTGGCTGTGA; GGCAGGACACGTGCTCCATTGTGGCTATCTCGCTGAACATCACACAGAAAGTCCATCCAGTCATCTGGTC; CCTCACCAGCTTGCCTTTTGACTGTACCCAGGCCCTGGCTGTGCCCAAGCCCATAGGTGGGGTAGTGATC; TTCGCTGTCAACTCACTGTTGTACCTGAACCAGAGTGTTCCCCCATATGGTGTGGCTCTCAATAGTCTCA; CCACAGGCACCACCGCTTTCCCATTGCGTACCCAAGAGGGTGTACGGATCACCCTAGACTGCGCACAGGC; GGCCTTCATCTCTTATGACAAGATGGTCATCTCCCTCAAGGGTGGTGAGATTTATGTGCTGACCCTCATC; ACTGACGGCATGCGAAGTGTCCGAGCGTTTCACTTTGACAAGGCAGCTGCTAGTGTCCTTACCACCAGTA; TGGTCACAATGGAGCCCGGATACCTGTTCCTAGGCTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC; GGAGAAGCTGCAGGAGCCCCCAGCCAGCTCCGTCCGTGAGGCTGCTGACAAGGAAGAGCCACCCTCAAAG; AAGAAGCGAGTGGAGCCTGCAGTGGGCTGGACAGGAGGCAAGACGGTGCCACAGGACGAGGTGGATGAAA; TTGAAGTTTATGGCAGTGAGGCCCAGTCAGGCACACAGCTGGCTACTTACTCTTTTGAGGTATGTGACAG; CATGCTCAACATTGGACCCTGTGCCAACGCTGCTGTGGGCGAGCCTGCCTTCCTCTCTGAAGAGTTCCAG; AATAGCCCTGAGCCAGACCTGGAGATTGTGGTTTGTTCCGGCTACGGGAAGAATGGGGCCCTGTCAGTGT; TGCAGAAGAGTATCCGGCCCCAGGTGGTGACAACCTTTGAACTTCCTGGCTGCTATGATATGTGGACAGT; CATTGCTCCTGTGCGGAAAGAGGAGGAAGAAACCCCCAAAGCAGAGAGCACAGAGCAGGAGCCCAGTGCC; CCGAAAGCAGAGGAGGATGGGCGAAGACATGGCTTCCTTATTCTTAGCCGGGAAGACTCAACCATGATCC; TACAGACTGGCCAGGAGATCATGGAGCTGGACACCAGTGGTTTTGCCACTCAGGGTCCTACTGTCTTTGC; TGGAAACATTGGGGACAATCGTTACATTGTTCAAGTCTCGCCACTGGGCATTCGCCTGTTGGAAGGCGTG; AATCAATTGCACTTCATCCCTGTGGACCTGGGCGCCCCCATCGTGCAGTGTGCCGTGGCTGATCCCTATG; TGGTTATCATGAGTGCTGAGGGACACGTTACCATGTTCCTACTCAAGAGCGATTCCTATGGTGGCCGTCA; TCACCGCCTGGCACTACACAAGCCTCCACTGCATCATCAGTCCAAGGTGATTGCTCTCTGCCTGTACCGC; GATGTCAGTGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGATGAACTGGGTGGCCGAAGTG; GCTCCGAGGCAGAAGGCCTGGGCTCAGAAACGAGCCCCACAGTGGATGATGAGGAAGAGATGCTCTATGG; GGACTCCAGTGCCCTCTTCAGTCCCAGCAAAGAGGAGGCCCGTAGGAGCAGCCAGCCACCGGCTGATCGT; GACCCTGCACCCTTCAAGGCAGACCCCACTCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCATGGAGA; TCTACCAGCTCCCAGACTGGCGGCTGGTATTCCTAGTTAAGAACTTCCCCGTGGGGCAGCGGGTTCTGGT; GGACAGTTCATTTGGACAGCCCACTACACAAGGTGAAGTCCGAAAGGAAGAAGCCACACGCCAGGGGGAG; CTGCCTTTGGTTAAGGAGGTGTTGTTGGTTGCTCTGGGAAGCCGGCAGAGCAGGCCCTACCTGCTGGTAC; ACGTGGACCAGGAGTTGCTTATCTATGAAGCCTTTCCCCATGACTCTCAGCTTGGCCAGGGAAATCTTAA; AGTCCGTTTCAAAAAGGTCCCTCACAATATCAACTTCCGTGAAAAGAAGCCAAAGCCGTCCAAGAAGAAG; GCCGAAGGTTGCAGCACTGAGGAGGGATCTGGAGGCCGGGGCCGTGTGGCACGTTTCCGCTACTTTGAGG; ATATTTATGGCTATTCGGGGGTTTTCATCTGTGGACCCTCACCTCACTGGCTCCTGGTGACTGGCCGCGG; AGCTCTGCGTCTGCACCCCATGGGCATTGATGGCCCCATTGACTCCTTTGCTCCTTTCCACAATGTGAAC; TGCCCCCGTGGTTTCCTGTACTTCAACAGACAGGGGGAGCTGAGGATCAGCGTCCTGCCTGCCTACCTGT; CCTACGATGCCCCATGGCCTGTCAGGAAGATCCCACTCCGATGCACAGCCCACTATGTGGCCTACCATGT; GGAATCCAAGGTCTATGCTGTGGCCACTAGTACCAACACACCTTGCACTAGGATCCCACGCATGACTGGC; GAGGAAAAAGAATTCGAGGCCATTGAGAGAGACGACAGATACATCCACCCCCAGCAAGAAGCCTTCTCCA; TCCAGCTCATCTCCCCGGTCAGCTGGGAGGCCATTCCCAATGCCAGGATCGAGCTAGAGGAATGGGAGCA; TGTCACGTGCATGAAGACAGTGTCACTGCGCAGTGAGGAGACCGTGTCAGGCCTAAAGGGCTATGTGGCT; GCTGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGTGGGCGGATCCTGATCATGGATGTGATTG; AAGTGGTGCCTGAGCCTGGGCAGCCCCTGACCAAGAACAAATTCAAGGTCCTGTATGAGAAGGAGCAGAA; AGGGCCCGTGACTGCGCTGTGCCACTGCAATGGCCACCTGGTGTCGGCCATTGGCCAAAAAATCTTCCTG; TGGAGCCTGCGGGCTAGCGAGCTGACCGGCATGGCCTTCATCGACACCCAGCTGTACATCCACCAGATGA; TCAGTGTCAAGAACTTCATCCTGGCAGCAGACGTCATGAAGAGCATCTCACTGCTGCGTTACCAAGAGGA; GAGCAAGACGCTGAGCCTGGTGTCCCGGGATGCCAAGCCTTTGGAGGTATACAGCGTGGACTTCATGGTG; GACAATGCCCAACTGGGCTTCTTGGTGTCTGACCGAGACCGCAATCTTATGGTGTATATGTATCTGCCAG; AAGCCAAAGAGAGTTTTGGGGGCATGCGCCTGCTACGCAGAGCGGATTTCCATGTGGGTGCCCACGTGAA; TACGTTCTGGAGGACCCCGTGCCGAGGTGCTGCCGAGGGCCCCAGCAAGAAGTCAGTCGTGTGGGAAAAC; AAGCATATCACATGGTTTGCAACCCTTGACGGTGGCATTGGGCTCCTGCTGCCCATGCAAGAAAAGACAT; ACCGACGCTTGCTGATGCTGCAGAATGCACTGACGACCATGCTACCCCACCACGCTGGCCTCAACCCCCG; CGCCTTCCGAATGTTGCACGTAGACCGGCGCATCTTGCAGAATGCTGTGCGCAACGTCCTTGATGGGGAA; CTGCTAAACCGCTACCTATACCTCAGCACCATGGAGCGCAGTGAGCTGGCCAAGAAGATAGGTACCACGC; CTGACATTATCCTGGATGACTTGCTGGAGACAGACCGGGTCACGGCTCACTTCTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053193
• Insert Size: 4326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_053193.2, NP_444423.1
• RefSeq Size: 4538 bp
• RefSeq ORF: 4326 bp
• Locus ID: 94230
• UniProt ID: Q9EPU4
• Cytogenetics: 15 D3
• Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.