Nos1 (NM_008712) Mouse Untagged Clone

SKU MC224449

Nos1 (untagged) - Mouse nitric oxide synthase 1, neuronal (Nos1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Nos1
• Synonyms: 2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224449 representing NM_008712; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGAGCACACGTTTGGGGTCCAGCAGATCCAACCCAACGTCATTTCTGTCCGTCTCTTCAAACGCA; AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCCCCGGTGATCATCTCAGATCTGAT; CCGAGGAGGTGCCGCGGAGCAGAGCGGCCTTATCCAAGCCGGCGACATCATTCTCGCCGTCAATGATCGG; CCCCTGGTAGACCTCAGCTATGACAGCGCTCTGGAAGTTCTCAGGGGCATTGCCTCTGAGACCCATGTGG; TCCTCATTCTGAGAGGCCCTGAGGGCTTTACTACACACCTGGAGACCACCTTCACAGGGGATGGAACCCC; CAAGACCATCCGTGTGACCCAGCCCCTGGGGACACCCACCAAAGCTGTCGATCTGTCTCGCCAGCCATCA; GCCAGCAAAGACCAGCCATTAGCAGTAGACAGGGTCCCAGGTCCCAGTAACGGACCTCAGCATGCCCAAG; GCCGTGGACAGGGAGCCGGCTCAGTCTCCCAGGCTAATGGTGTGGCCATTGACCCCACAATGAAAAATAC; CAAGGCCAACCTCCAGGACAGCGGAGAACAGGATGAACTGCTCAAAGAGATAGAACCTGTGTTGAGCATC; CTCACCGGTGGGGGAAAAGCAGTCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC; AGGTGGACAGAGACCTCGACGGCAAACTGCACAAAGCTCCGCCCCTGGGCGGGGAGAATGATCGAGTCTT; CAATGATCTGTGGGGGAAGGGCAACGTCCCTGTGGTCCTCAACAACCCGTATTCAGAGAACGAACAGTCT; CCCGCCTCGGGCAAACAGTCTCCTACCAAGAATGGCAGTCCTTCCAGGTGCCCTCGCTTCCTTAAGGTCA; AGAACTGGGAGACAGATGTGGTCCTCACTGACACCCTGCACCTGAAGAGCACGCTGGAAACGGGGTGCAC; AGAGCAGATCTGCATGGGCTCCATCATGCTGCCATCCCATCACATACGGAAGTCAGAAGATGTCCGCACC; AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCCTCCATTAAGAGGTTTGGCT; CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATCGAGAGCACCAGCACCTACCAGCTCAA; GGACACGGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGCTGTGTGGGCAGGATCCAG; TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCATGGGATGTTCAACTACATCTGTA; ACCACGTCAAGTACGCCACCAACAAAGGGAACCTCAGGTCGGCCATCACTATATTCCCTCAAAGGACTGA; TGGCAAGCATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTATGCCGGCTACAAGCAGCCAGATGGC; TCTACCTTGGGCGATCCAGCTAATGTGGAGTTCACAGAGATCTGTATACAGCAGGGCTGGAAACCCCCAA; GAGGCCGGTTCGATGTGCTGCCTCTCCTGCTCCAGGCCAATGGCAATGACCCTGAACTCTTTCAGATCCC; CCCAGAGCTGGTGCTGGAAGTACCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA; TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATTGGGGGCCTGGAGTTCAGCGCCTGTCCCT; TTAGTGGCTGGTACATGGGCACGGAGATTGGCGTTCGTGATTACTGTGACAACTCTCGATACAACATCCT; GGAGGAAGTAGCCAAGAAAATGGATTTGGACATGAGGAAGACATCCTCCCTCTGGAAGGACCAAGCCCTG; GTGGAGATTAACATTGCTGTCCTATACAGCTTCCAGAGTGACAAGGTGACCATTGTGGACCACCACTCTG; CCACCGAGTCCTTCATCAAACACATGGAGAATGAGTACCGCTGCAGAGGGGGCTGTCCCGCCGACTGGGT; GTGGATTGTGCCCCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTACCGGCTC; ACCCCGTCCTTTGAGTACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAATGGGACCCCCA; CGAAGCGGCGAGCCATTGGCTTCAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTGATGGGACA; GGCCATGGCCAAGAGAGTCAAAGCGACCATTCTCTACGCCACCGAGACAGGCAAATCCCAAGCCTATGCC; AAGACCCTGTGCGAGATCTTCAAGCATGCCTTCGATGCCAAGGCTATGTCCATGGAGGAATATGACATCG; TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCAGAGAA; TGGGGAGAAATTCGGCTGTGCTTTGATGGAGATGAGGCACCCCAACTCTGTGCAAGAGGAGAGGAAGAGC; TACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGACTCCCGCAAGTCATCAGGCGATGGACCAGACC; TCAGAGACAACTTTGAAAGTACTGGACCGCTGGCCAATGTGAGGTTCTCAGTCTTCGGCCTGGGCTCTCG; GGCATACCCTCACTTCTGTGCCTTTGGGCACGCAGTGGACACTCTCCTGGAGGAGCTGGGCGGGGAGAGG; ATTCTGAAGATGAGGGAGGGAGATGAGCTCTGCGGACAGGAAGAGGCTTTCAGGACCTGGGCTAAGAAAG; TCTTCAAGGCAGCCTGTGATGTGTTCTGTGTGGGGGATGACGTCAACATTGAGAAGGCGAACAATTCCCT; CATTAGCAATGACCGCAGCTGGAAGAGGAATAAATTCCGCCTCACTTATGTGGCAGAAGCTCCAGAGCTG; ACCCAAGGTCTTTCCAATGTTCACAAAAAGCGAGTCTCAGCCGCCCGACTCCTCAGCCGCCAAAACCTGC; AAAGTCCTAAATCCAGCCGATCGACCATCTTCGTGCGTCTCCACACCAACGGGAATCAGGAGTTGCAGTA; CCAGCCAGGGGACCACCTGGGTGTCTTCCCTGGCAACCATGAGGACCTCGTGAATGCACTCATTGAACGG; CTGGAGGACGCGCCGCCTGCCAACCACGTGGTGAAGGTGGAAATGCTGGAGGAAAGGAACACGGCTCTGG; GTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCACCCTGCACCATCTTCCAGGCCTTCAAGTACTA; CCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGCAGCAGTTCGCCTCCCTGGCCACCAATGAGAAA; GAGAAGCAGCGGCTGCTGGTCCTCAGCAAGGGGCTTCAGGAATATGAGGAATGGAAATGGGGCAAGAACC; CCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATCCAGATGCCGGCCACACTTCTCCTCACACAGCT; GTCGCTGCTACAACCTCGCTACTATTCCATCAGCTCCTCTCCAGACATGTACCCCGACGAGGTGCACCTC; ACCGTGGCCATCGTGTCCTACCATACCCGAGATGGAGAAGGACCGGTTCACCACGGGGTGTGCTCCTCCT; GGCTCAACCGAATACAGGCTGACGATGTCGTCCCCTGCTTCGTGAGAGGTGCCCCTAGCTTCCACCTGCC; TCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAGGCACCGGCATCGCTCCCTTCCGAAGTTTTTGG; CAACAGCGACAATTTGACATCCAACACAAAGGAATGAATCCGTGCCCCATGGTTCTGGTCTTCGGGTGTC; GACAATCCAAGATAGATCATATCTACAGAGAGGAGACCCTGCAGGCCAAGAACAAGGGCGTCTTCAGAGA; GCTGTACACTGCCTACTCCCGGGAACCCGACAGGCCAAAGAAATATGTACAGGACGTGCTGCAGGAACAG; CTGGCCGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGGAGGCCATATTTATGTCTGCGGGGACGTTACCA; TGGCCGCTGATGTCCTCAAAGCCATCCAGCGCATAATGACCCAGCAGGGGAAACTCTCGGAGGAGGATGC; TGGTGTGTTCATCAGCAGGCTGAGGGATGACAACCGGTACCACGAGGACATCTTTGGGGTCACCCTCAGA; ACATATGAAGTGACCAACCGCCTTAGATCTGAGTCCATCGCCTTCATTGAAGAGAGCAAAAAAGACACAG; ATGAGGTTTTCAGCTCCTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008712
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008712.3, NP_032738.1
• RefSeq Size: 9695 bp
• RefSeq ORF: 4290 bp
• Locus ID: 18125
• UniProt ID: Q9Z0J4
• Cytogenetics: 5 57.29 cM
• Summary: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex.[UniProtKB/Swiss-Prot Function]