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Kdm6a (NM_009483) Mouse Untagged Clone

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SKU
MC224431
Kdm6a (untagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,828.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Kdm6a
Synonyms Utx
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224431 representing NM_009483
Blue=ORF Red=Cloning site Green=Tag(s) Orange=Stopcodon

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCCGCCGCCGCCGCCGCCGCTTTC
GGTGATGAGGAAAAGAAAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCC
CCCAGCCTGACAGCGGAGGAGAGGGAGGCGCTCGGCGGACTGGACAGCCGCCTTTTCGGG
TTCGTGAGGTTTCATGAAGATGGCGCCAGGATGAAGGCCCTGCTGGGCAAGGCTGTTCGC
TGCTACGAATCTCTAATCTTAAAAGCTGAAGGGAAAGTGGAGTCTGATTTCTTTTGTCAA
TTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCAGAGG
TACTACAGTTTACAGTCTGATTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTG
GTCTACTTCCATTACAATGCATTTCAGTGGGCTATTAAAGCATTTCAGGAGGTGCTTTAT
GTAGATCCCAGCTTTTGTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAA
GTGAACACAGACTATGAGTCTAGTTTAAAGCATTTTCAGTTAGCTTTGGTTGACTGTAAT
CCCTGCACTTTGTCCAATGCTGAAATTCAGTTTCACATTGCCCACTTATATGAAACCCAG
AGGAAGTATCATTCTGCAAAAGAAGCTTATGAGCAACTTTTGCAGACAGAAAACCTTTCT
GCACAAGTAAAAGCAACTATTTTACAACAATTAGGTTGGATGCATCACACTGTGGATCTC
CTGGGAGATAAGGCCACCAAGGAAAGTTATGCTATTCAGTATCTCCAGAAGTCCTTGGAA
GCAGATCCAAATTCTGGCCAGTCCTGGTATTTCCTTGGAAGGTGCTATTCAAGTATTGGG
AAAGTTCAGGATGCCTTTATATCTTACAGGCAATCTATTGATAAATCAGAAGCAAGTGCA
GATACATGGTGTTCAATAGGTGTGCTCTATCAACAGCAAAATCAGCCTATGGATGCTTTG
CAAGCTTATATTTGTGCTGTACAATTGGACCACGGTCATGCTGCAGCCTGGATGGATCTA
GGCACTCTCTATGAATCCTGCAACCAACCTCAGGATGCTATTAAATGCTATTTAAATGCA
ACTAGAAGCAAAAATTGTAGTAATACCTCTGGACTTGCAGCACGAATTAAGTATTTACAG
GCTCAGTTGTGTAACCTTCCACAAGGTAGTCTACAGAATAAAACTAAATTACTTCCTAGT
ATTGAGGAGGCATGGAGCCTACCAATCCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATG
AACACAGCACAGCAGAATACTTCTGATAATTGGAGTGGTGGCAATGCACCACCTCCAGTA
GAACAACAAACTCATTCATGGTGTTTGACACCACAGAAATTACAGCACTTGGAACAGCTC
CGAGCAAACAGAAATAATTTAAATCCAGCACAGAAACTAATGCTGGAACAGCTGGAAAGT
CAGTTTGTCTTAATGCAGCAACACCAAATGAGACAAACAGGAGTTGCACAGGTACGGCCT
ACTGGAATTCTTAATGGGCCAACAGTTGACTCATCACTGCCTACAAACTCAGTTTCTGGC
CAGCAGCCACAGCTTCCTCTGACCAGAATGCCTAGTGTCTCTCAGCCTGGAGTCCACACT
GCCTGTCCTAGGCAGACTTTGGCCAATGGACCCTTTTCTGCAGGCCATGTTCCCTGTAGC
ACATCAAGAACACTGGGAAGTACAGACACTGTTTTGATAGGCAATAATCATGTAACAGGA
AGTGGAAGTAATGGAAACGTGCCTTACCTGCAGCGAAACGCACCCACTCTACCTCATAAC
CGCACAAACCTGACCAGCAGCACAGAGGAGCCGTGGAAAAACCAACTATCTAACTCCACT
CAGGGGCTTCACAAAGGTCCGAGTTCACATTTGGCAGGTCCTAATGGTGAACGACCTCTA
TCTTCCACTGGGCCTTCCCAGCATCTCCAGGCAGCTGGCTCTGGTATTCAGAATCAGAAT
GGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGGGCTGCTCTCAATCACCTCTCC
TCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAGAGAGCAAGCCT
TCAGGAAACACATTGACGGTGCCTGAAACAAGCAGGCAAACTGGAGAGACACCTAACAGC
ACTGCCAGTGTTGAGGGACTTCCTAATCATGTCCATCAGGTGATGGCAGATGCTGTTTGC
AGTCCTAGCCATGGAGATTCTAAGTCACCAGGTTTACTAAGTTCAGACAATCCTCAGCTC
TCTGCCTTGTTGATGGGAAAAGCTAATAACAATGTGGGTCCTGGAACCTGTGACAAAGTC
AATAACATCCACCCAACTGTCCATACAAAGACTGATAATTCTGTTGCCTCTTCACCATCT
TCAGCCATTTCCACAGCAACACCTTCTCCTAAGTCCACTGAACAGACAACCACAAACAGT
GTTACCAGCCTTAACAGCCCTCACAGTGGGCTGCACACAATTAATGGAGAAGGAATGGAA
GAATCTCAGAGCCCCATTAAAACAGATCTGCTTCTAGTTAGCCACAGACCTAGTCCTCAG
ATCATACCATCAATGTCTGTGTCCATATATCCCAGCTCAGCAGAAGTTCTGAAAGCTTGC
AGGAATCTAGGTAAAAACGGCCTGTCTAATAGTAGCATTCTGTTGGATAAATGTCCGCCT
CCAAGACCACCATCCTCACCATACCCTCCCTTGCCAAAGGACAAGTTGAATCCACCTACA
CCTAGTATTTATTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACATCAATTTTGT
ACAAACCCAAACAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTAGAC
TTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATATGGTAGAAGTG
AGGACACAGTTGTTACAACCAGCAGATGAAAATTGGGACCCTACTGGAACCAAGAAAATC
TGGCACTGTGAAAGTAATAGATCTCATACTACAATTGCTAAATATGCTCAGTACCAGGCC
TCCTCATTCCAAGAATCATTGAGAGAAGAAAATGAGAAAAGAAGTCACCATAAAGACCAC
TCAGACAGTGAATCTACATCATCAGATAATTCTGGGAAAAGAAGAAAAGGACCCTTTAAA
ACCATTAAGTTTGGGACCAACATTGACCTGTCTGATGACAAAAAGTGGAAGTTACAGCTA
CATGAGCTGACTAAACTTCCTGCCTTCGTGAGAGTTGTATCTGCAGGAAATCTTTTAAGC
CACGTTGGTCATACTATACTGGGCATGAACACAGTTCAACTATACATGAAAGTTCCAGGA
AGCAGAACACCAGGTCATCAAGAAAATAACAACTTCTGTTCAGTTAATATAAATATTGGC
CCAGGTGACTGTGAATGGTTTGTTGTTCCTGAAGGCTACTGGGGTGTTTTGAATGACTTC
TGTGAAAAAAATAATTTGAATTTCTTAATGGGTTCTTGGTGGCCCAACCTTGAAGATCTA
TATGAAGCAAATGTTCCAGTGTATAGGTTTATTCAGCGACCTGGAGATCTGGTCTGGATA
AATGCTGGCACTGTTCATTGGGTTCAAGCTATTGGCTGGTGCAACAATATTGCTTGGAAT
GTTGGTCCACTTACAGCCTGTCAGTATAAGTTAGCAGTGGAACGTTATGAATGGAACAAG
TTGCAAAATGTAAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATATGGCACGAAAT
ATCAAGGTTTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTGAGAACGCTG
AAGCAATGTCAGACATTGAGGGAAGCTCTAATTGCTGCAGGAAAAGAGATCATATGGCAC
GGGCGGACAAAAGAAGAACCAGCTCATTATTGTAGTATTTGTGAGGTGGAGGTTTTTGAT
CTGCTTTTTGTCACTAATGAGAGTAATTCTCGAAAAACCTACATAGTACATTGCCAAGAT
TGTGCACGAAAAACAAGTGGGAATCTGGAAAATTTTGTGGTGCTAGAACAGTACAAAATG
GAGGATCTGATGCAAGTCTATGACCAATTTACATTAGTAAGTGAAATCAACATGCTCCTC
CATTACCATCCGCCTCATCTTGATATTGTTCCATGGACATTAAACATGAGACCTTTTCTG
CTATTCAGAAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTG
GATTACAAGGATGACGACGATAAGGTTTAAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_009483
Insert Size 4275 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009483.1, NP_033509.1
RefSeq Size 5309 bp
RefSeq ORF 4275 bp
Locus ID 22289
UniProt ID O70546, Q3TPN3
Cytogenetics X 13.45 cM
Summary Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (By similarity). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (PubMed:21095589).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
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"NM_009483" in other vectors (6)
SKU Description Size Price
MG211981 Kdm6a (tGFP-tagged) - Mouse ubiquitously transcribed tetratricopeptide repeat gene, X chromosome (Utx) 10 ug
$2,027.00
MR211981 Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) 10 ug
$1,827.00
MR211981L1 Lenti ORF clone of Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) 10 ug
$2,127.00
MR211981L2 Lenti ORF clone of Kdm6a (mGFP-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) 10 ug
$2,127.00
MR211981L3 Lenti ORF clone of Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) 10 ug
$2,127.00
MR211981L4 Lenti ORF clone of Kdm6a (mGFP-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a) 10 ug
$2,127.00

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