Grin2d (NM_008172) Mouse Untagged Clone

SKU MC224232

Grin2d (untagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Grin2d
• Synonyms: GluN2D; NMDAR2D; NR2D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224232 representing NM_008172; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCGCGGCGCCGGTGGCCCCCGCGGCCCTCGGGGCCCCGCTAAGATGCTGTTGCTGCTGGCGCTGGCGT; GCGCCAGCCCGTTCCCGGAGGAGGTGCCGGGGCCGGGCGCGGCCGGCGGGGGCACGGGCGGGGCGCGGCC; GCTCAACGTGGCGCTGGTCTTCTCTGGCCCGGCGTACGCGGCCGAGGCGGCGCGCTTGGGCCCGGCCGTG; GCGGCGGCAGTGCGCAGCCCGGGCCTGGACGTACGGCCCGTGGCGCTGGTGCTCAACGGCTCCGACCCTC; GCAGCCTTGTGCTGCAGCTCTGCGACCTGCTGTCGGGGCTGCGCGTGCACGGCGTGGTGTTCGAGGACGA; CTCGCGCGCGCCCGCCGTCGCGCCCATTCTCGACTTCCTGTCGGCGCAGACCTCGCTGCCCATCGTGGCC; GTGCACGGCGGCGCCGCGCTCGTACTCACACCCAAGGAGAAGGGCTCCACCTTCCTGCAGCTTGGCTCCT; CCACAGAGCAACAGCTGCAGGTCATTTTTGAGGTGCTGGAGGAGTACGACTGGACATCCTTTGTGGCAGT; GACTACGCGTGCCCCAGGCCATCGAGCCTTCTTGTCATACATCGAGGTGCTGACTGATGGCAGCCTGGTG; GGCTGGGAGCATCGAGGAGCGCTGACACTGGACCCCGGAGCGGGTGAGGCCGTCCTGGGCGCACAGCTCC; GTAGTGTCAGTGCGCAGATCCGCCTGCTCTTCTGCGCCCGCGAGGAGGCAGAGCCTGTTTTCCGGGCGGC; AGAAGAGGCTGGTCTCACTGGGCCTGGCTACGTCTGGTTCATGGTGGGACCTCAGCTGGCCGGAGGTGGG; GGCTCCGGGGTCCCTGGGGAACCACTTCTTCTGCCAGGAGGTGCCCCACTGCCTGCTGGGCTGTTTGCAG; TGCGCTCTGCTGGCTGGCGTGACGACTTGGCACGTCGAGTGGCTGCTGGTGTGGCGGTGGTGGCCAGAGG; TGCCCAGGCCCTGCTGCGAGACTATGGCTTCCTGCCTGAGCTGGGCCATGACTGTCGCGCCCAGAATCGC; ACCCACCGCGGGGAGAGTCTGCACAGGTATTTCATGAACATCACCTGGGATAACCGAGACTACTCCTTCA; ATGAGGATGGCTTTCTGGTAAACCCGTCACTGGTAGTCATCTCCCTCACCAGAGACAGGACGTGGGAAGT; GGTGGGCAGCTGGGAACAGCAGACCCTCCGCCTCAAGTACCCTCTATGGTCCCGCTATGGCCGCTTCCTG; CAGCCGGTGGATGACACGCAGCACCTCACTGTGGCCACGCTGGAGGAGAGACCTTTTGTCATTGTAGAGC; CTGCAGACCCCATCAGCGGCACTTGCATCAGAGACTCGGTTCCCTGCCGGAGCCAGCTCAACCGTACCCA; CAGCCCTCCGCCTGACGCTCCCCGCCCGGAGAAGAGATGCTGCAAGGGTTTCTGCATTGACATTTTGAAG; AGGCTGGCGCACACCATCGGTTTCAGCTATGACCTCTACCTGGTTACCAACGGCAAGCATGGCAAGAAGA; TCGATGGCGTCTGGAATGGCATGATTGGTGAGGTGTTCTATCAGCGTGCGGACATGGCCATCGGCTCCCT; CACCATCAATGAGGAGCGGTCAGAGATCGTGGACTTCTCCGTCCCTTTTGTAGAGACAGGCATCAGCGTC; ATGGTGGCACGCAGCAATGGCACTGTGTCCCCCTCTGCCTTCCTCGAGCCCTACAGCCCCGCTGTGTGGG; TGATGATGTTCGTCATGTGCCTCACCGTGGTCGCCGTCACAGTTTTCATCTTTGAGTACCTCAGTCCTGT; GGGCTATAACCGAAGCCTGGCCACGGGCAAACGCCCCGGAGGCTCTACCTTCACCATTGGGAAATCCATC; TGGCTGCTGTGGGCCCTGGTGTTCAACAACTCCGTGCCAGTGGAGAATCCTCGGGGCACCACCAGCAAGA; TCATGGTGCTGGTGTGGGCCTTCTTTGCCGTCATCTTTCTTGCCAGCTATACAGCCAATCTGGCTGCCTT; CATGATCCAGGAGGAGTACGTGGACACCGTGTCTGGGCTCAGCGACCGGAAGTTCCAGCGGCCCCAGGAG; CAATACCCACCCCTGAAGTTTGGAACGGTGCCCAATGGGTCCACGGAGAAGAATATCCGAAGCAACTACC; CTGATATGCACAGCTACATGGTGCGATACAACCAGCCAAGAGTGGAGGAGGCGCTCACTCAGCTCAAGGC; AGGGAAACTGGACGCCTTCATCTATGACGCAGCAGTGCTCAACTACATGGCCCGAAAGGATGAGGGCTGC; AAGCTGGTCACCATCGGCTCAGGCAAGGTCTTCGCCACCACTGGTTATGGCATCGCCCTACACAAGGGCT; CCCGCTGGAAGAGGCCCATCGACCTGGCGCTGCTGCAGTTCCTGGGGGACGATGAGATTGAGATGCTGGA; GCGGCTGTGGCTTTCAGGGATCTGCCACAACGACAAAATCGAGGTGATGAGCAGCAAGCTGGATATCGAC; AACATGGCAGGTGTCTTCTACATGCTCCTCGTGGCCATGGGCCTCTCCTTGCTGGTCTTCGCCTGGGAAC; ACCTTGTGTACTGGCGACTGCGGCACTGTCTGGGGCCCACCCACCGCATGGATTTCCTACTGGCCTTCTC; CAGGGGTATGTACAGCTGCTGCAGCGCTGAGGCTGCTCCGCCGCCGGCCAAACCCCCGCCACCGCCGCAG; CCGCTGCCCAGTCCGGCGTATCCCGCCGCTCGCCCACCCCCTGGCCCCGCACCCTTCGTGCCCCGAGAGC; GCGCAGCCGCCGACCGCTGGCGCCGGGCCAAGGGCACAGGGCCCCCGGGGGGCGCAGCGCTAGCCGACGG; CTTCCACCGATACTACGGCCCCATCGAGCCGCAGGGGCTGGGCCTCGGCGAGGCGCGCGCGGCACCGAGA; GGCGCAGCCGGACGCCCACTGTCCCCACCCACCACACAGCCCCCACAGAAGCCACCACCTTCCTACTTCG; CCATCGTGCGCGAGCAAGAGCCGGCCGAGCCCCCCGCCGGCGCCTTCCCGGGCTTCCCATCTCCGCCCGC; TCCGCCTGCCGCCGCAGCCGCCGCCGTCGGGCCGCCACTGTGCCGCCTGGCTTTCGAGGACGAGAGCCCG; CCCGCGCCCTCGCGCTGGCCGCGTTCTGACCCCGAGAGCCAGCCGCTGTTGGGTGGGGGCGCGGGCGGCC; CGAGCGCTGGGGCCCCGACCGCACCACCGCCGCGCCGCGCCGCGCCACCACCGTGCGCCTACCTGGACCT; CGAGCCTTCGCCTTCGGACTCCGAGGATTCGGAGAGCCTGGGCGGAGCGTCGCTCGGTGGCCTGGAGCCC; TGGTGGTTCGCCGACTTCCCCTACCCGTATGCGGAGCGCCTCGGGCCGCCGCCCGGCCGCTACTGGTCGG; TTGACAAGCTCGGGGGCTGGCGCGCTGGTAGCTGGGACTACCTGCCCCCGCGCGGCGGCCCCGCATGGCA; CTGCCGCCACTGCGCCAGCCTGGAGCTGCTACCGCCACCACGCCATCTCAGCTGCTCGCACGACGGCCTA; GACGGTGGCTGGTGGGCGCCTCCGCCTCCACCCTGGGCTGCGGGGCCACCGCCCCGGCGCCGGGCGCGCT; GTGGTTGTCCGCGACCGCACCCGCACCGCCCCCGGGCTTCGCACCGTGCGCCCGCCGCCGCACCGCACCA; CCACCGACACAGGCGCGCGGCGGGTGGCTGGGACCTCCCGCCGCCCGCGCCCACCTCGCGTTCGCTGGAG; GACCTGAGCTCCTGCCCACGGGCGGCCCCCACGCGCAGGCTCACCGGGCCCTCGCGCCACGCGCGCCGCT; GTCCGCACGCTGCGCATTGGGGGCCGCCCCTGCCCACCGCATCTCACCGGAGACACCGGGGCGGGGACCT; GGGCACACGCAGGGGCTCTGCGCACTTCTCCAGCCTGGAGTCCGAGGTATGA; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008172
• Insert Size: 3972 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008172.2, NP_032198.2
• RefSeq Size: 4383 bp
• RefSeq ORF: 3972 bp
• Locus ID: 14814
• UniProt ID: Q03391
• Cytogenetics: 7 29.54 cM
• Summary: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1385220). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable).[UniProtKB/Swiss-Prot Function]