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Tbc1d32 (NM_001033385) Mouse Untagged Clone

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SKU
MC224194
Tbc1d32 (untagged) - Mouse RIKEN cDNA D630037F22 gene (D630037F22Rik), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,242.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tbc1d32
Synonyms b2b2284Clo; Bromi; C6orf170; D630037F22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224194 representing NM_001033385
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCATTTCTCCAGCGAGGATGAGGTGATGCTGCAGGCGATGCTCCGGCAGCTGTTCCAGAGCGTGA
AGGAGAAAATCACCGGAGCTCCCTCCTTGGAGTGCGCAGAGGAGATCCTCTTGCGGCTGGAGGAGACTGA
CGAAAATTTTCACAACTATGAATTTGTGAAATACCTCAGACAGCACATATGTAACATGTTGGGGTCTATG
ATTGAAGAAGAAATGGAAAAATGCACATCTGATCAGAATCAGGGGGAGGATTCTGGCTATGACACGGTTG
TTCAGCATGTTACCAAAAGAACGCAGGAATCTAAAGAGTATAAGGAAATGATGCATTCCCTGAAGAACAT
TATGATGGTGGTGGTCGAGGCGATGATCAACAAGTTTGAGGAAGACGAGACACGGAGTGAGGACAGGCAG
AGGAAAATGCAGAGTGGCAGCTGCTGCACAGACAACTGCTCCGACAGTGACTCCTCCTTCAATCAGAGTT
ACAAATTCTGTCAAGGGAAACTACGGTTGATTTTAGACCAGTTGGATCCTGGCCAACCTAAAGAGGTGAG
ATATGAAGCTTTACAAACATTGTGCTCAGCTCCCCCATCCGACGTCCTGAGCTGTGAGAACTGGACCACT
CTCTGTGAAAAGCTGACCACATCTCTGTCTGATCCAGATCCTATGTTCACTGACCGGATTTTAAAGTTCT
ACGCACAGACTTTTACCCTCTCACCGTTACATATGACCAAGGAGATTTATACAAGCTTAGCTAAATATCT
GGAGGTATATTTTCTTTCTAGAGAAAATCACCTTCCTACTCTTTCAACTGGAGTAGACATAACCAGTCCC
AATGTGACCCGCTTACTGAAAAAGGTTCGCCTTCTGAATGAGTACCAGAAAGAGGCTCCATCTTTTTGGA
TTCGTCACCCAGAGAAGTATATGGAGGAAATTGTGGAGAGTACTCTGTCCTTGCTCTCAGTCAAACACGA
GCAAAGCCACCTGGTGCCACAGAAGATTTTGGATCCCATCTATTTTTTCGCATTAGTTGATACTAAGGCT
GTGTGGTTCAAAAAGTGGATGCATGCATATTACAGCAGAACTGCAGTACTGAGACTTCTTGAAAAGAAAT
ATAAATGTCTGATAACTACAGCAGTTCAGCAATGTGTTCAGTACTTGGAGTTATGTGAAGCTATGAAAGC
TGATGAAATTCTGAGACATCCAAAACATTGTGGAACCAAGCAAAAGAGTTTCTACTATTCAGGACAAGAA
TTGCAATATATTTATTTCATTCACTCACTGTGCCTTTTAGGAAGATTGTTGATCTATACACAGGGCAGGA
AATTATTCCCCATAAAGCTGAAGAATAGAAAAGATTCAGTATCCCTGACAAATCTGCTGGTTTTGTTTAC
TCAACTTATCTATTATTCACCAAGTTGTCCAAAGATGACCTCCATCATGTGTTCAGAGAACTATTCTCCT
GCAAGTATGGTGACTGACGTTCTGCGGATGCTCTGCGACCAGAAAGAATGTGCAGTCGAGTGCTTATACA
ACAGCACAGTGACAGAGGCGCTTCTCCTGCCCATTCATAACCTGACGAAAGGAACTGCGGCTGCTCCAGA
TTGCTCTGAGACAGCTCTAATTCATATAGCTGATATTTTGGCAAGAATCGCTTCTGTCGAGGAAGGACTC
ATCTTACTTCTGTATGGAGAAAATATGAACTCTTCTGAAGAAGAAAGTCTTACAGGTGCTCATATAATAG
CCAAGTTTTCAAAGAAACTTCTTGAAGAAGATATTTCTATTTTTTCTGGATCGGAAATGTTGCCTGTAGT
TAAAGGAGCTTTTATTTCTGTGTGTCGTCAGATCTACGGTACCTGCGAAGGCTTACAGGTGTTGCTTCCT
TATGGTTTACATGAATCCATAGCAAAGGCATGGAAGAAGACAAGCTTGCTATCAGAAAGGATCCCCACTC
CAGTAGAGGGCTCTGATTCTGTTTCCTCAGTGAGCCAGGTGTCTCCGAACAGTGTGGCTTGGGAAGACAA
TTTGTTGGATGATTTACTGAATTTTGCAGCCACCCCCAAAGGACTGCTGCTTCTTCAGAGAACAGGCGCC
ATCAATGAATGTGTGACGTTTATGCTCAGCCAGTATGCAAAAAAGCCACAGGTCAACAGGCAGAAGAAGT
TTGGCTATGAAGTCTTGGTTGTACAGGTGGCATCGACAGCAGCAGGTGCAGTGGCTCTACAGAATTCAGG
CTTTATTAGTGCACTTATAACTGAGTTATGGTCCAACCTTGAATGTGGAAGAGATGATGTAAGGCTAACT
CATCCCAGAGCTACCCCAGTGGACCCAATTGACCGAAGCTGTCAGAAGTCCTTCCTTGCACTGGTGAACC
TGCTGTCCTACCCTGCTGTGTATGAGCTAACAGCCAATCAGGAGCTTCCTAATAAGGCAGAGTATTCTCT
TCGTGAAGTTCCCACATGCATCATCGATATAATGGATAGGCTTATTGTTTTGAACTCTGAAGCCAAGATC
CGGTCTTTACTGAATTATGAACAGTCGCACACCTTTGGTCTAAGGTTACTAAGTGTGGTGTGCTGTGATC
TGGATGCGCTTCTCTTACTGGAGGCTCAGTATCAAGTATCTAACATGTTATTACATGCTCAAGAAGAAAA
TACCTTTGAGATCTCTGAGAACCACAGGAACTTTATAATTGATGGATTATCAGTAGAGAGAAATCATGTT
CTCGTAAGGATTAATCTTATTGGTGGTCCTTCAGAGCGGATATTGCCTCCAAGGATGCTTGAGAAGGGGG
ATGACCCATATCCTTGGCCAATGTTTTCTTCATATCCATTGCCACACTGCTATCAGTCAGAAGGTCCACG
AAGTGCTGATCTAAAACAAGACAATGATATTGGAAACCTGTTATCATGCTTCAAAATGTCCGATAAACAA
ACAGAGTGGATAGAGAACTGCCGAAGACAGTTTTGTAAAACAATGAAGTCCAAGCCTGATGCAGTCCATG
GAAGTGCCTTAGGAGAACTACTGGAAAAATTTGTGCTGCTTCTCACCGAAAACCCATCTGAATGTTACTT
CCCTTCAGTGGAATACACAGCTACTGATGCAAATGTGAAGAATGAAAGTCTCTCATCTGTGCAGCAGCTT
GGCATGAAAATGACTGTCAGGTATGGGAGGTTTCTCAATTTGTTAAAAGATGGTGCAGAAAATGAGCTTG
CTTTGGTCTTAAAGCATTGTGAGAAATTCCTGAAACAACAGCAGTCACCGGTCACTTCTTCTCTCCTGTG
CCTGCAAGGGAACTACGCAGGCCATGACTGGTTTGTGTCTTCTCTGTTCATGATAATGCTGGGAGACAAA
GGGAAGACCTTCCACTTTCTCCAGCATTTCTCCAGGCTGCTGACCTCTGCCTTCCTTTGGGTGCCACGGC
TGCATAATTCTAGGTACCTTCCTGTCGACACCCTAGGGACCGGCATCCATCCCATCTACTTTTGCAGTGC
TCACTACATTGAAATGCTGCTGAAGGCTGAGGTGCCGCTTGTGTTTTCGGCTTTCCACATGTCTGGTTTT
GCTCCATCCCAGATTTGCCTGCAGTGGATAACTCAGTGTTTTTGGAATTACTTAGATTGGATAGAAATAT
GCCACTATATTGCTACTTGTGTTGTCCTTGGTCCTGATTATCAAGTGTATGTCTGCATAGCCGTTCTCAA
ACATCTGCAGCGAGATATTCTCCAGCATACCCAGACTCAAGATCTGCAAGTCTTTCTCAAAGAAGAAGCA
CTGCATGGGTTTCGAGTGAGCAACTATTTTGAATACATGGAGAATTTGGAGCAAAACTACCGACCAGTGC
TGCTGCGAGACATGCGCAGCATCAGAGTGCAGAACACATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001033385
Insert Size 3891 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033385.3, NP_001028557.2
RefSeq Size 7269 bp
RefSeq ORF 3891 bp
Locus ID 544696
UniProt ID Q3URV1
Cytogenetics 10 B3-B4
Summary Required for high-level Shh responses in the developing neural tube. Together with CDK20, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to Shh signaling.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033385" in other vectors (4)
SKU Description Size Price
MG220160 D630037F22Rik (tGFP-tagged) - Mouse RIKEN cDNA D630037F22 gene (D630037F22Rik), (10ug) 10 ug
$1,328.00
MR220160 D630037F22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630037F22 gene (D630037F22Rik) 10 ug
$589.00 $1,128.00
MR220160L3 Lenti ORF clone of D630037F22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630037F22 gene (D630037F22Rik) 10 ug
$1,428.00
MR220160L4 Lenti ORF clone of D630037F22Rik (mGFP-tagged) - Mouse RIKEN cDNA D630037F22 gene (D630037F22Rik) 10 ug
$1,428.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.