Prdm16 (NM_027504) Mouse Untagged Clone
SKU
MC224134
Prdm16 (untagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 1, (10ug)
$1,222.00
6 Weeks*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Prdm16 |
| Synonyms | 5730557K01Rik; csp1; mel1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-NotI | |
| ACCN | NM_027504 |
| Insert Size | 3828 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_027504.3, NP_081780.3 |
| RefSeq Size | 8605 bp |
| RefSeq ORF | 3828 bp |
| Locus ID | 70673 |
| UniProt ID | A2A935 |
| Cytogenetics | 4 E2 |
| Summary | Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.UniProtKB/Swiss-Prot Function Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_027504" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG222596 | Prdm16 (tGFP-tagged) - Mouse PR domain containing 16 (Prdm16) transcript variant 1, (10ug) | 10 ug |
$1,310.00
|
|
| MR222596 | Prdm16 (Myc-DDK-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 1 | 10 ug |
$589.00
$1,110.00
|
|
| MR222596L3 | Lenti ORF clone of Prdm16 (Myc-DDK-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 1 | 10 ug |
$1,410.00
|
|
| MR222596L4 | Lenti ORF clone of Prdm16 (mGFP-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 1 | 10 ug |
$1,410.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
