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Cdk12 (NM_026952) Mouse Untagged Clone

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SKU
MC224091
Cdk12 (untagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 3, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,205.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cdk12
Synonyms 1810022J16Rik; AI646528; Crk7; Crkrs; D11Ertd752e; Pksc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224091 representing NM_026952
Red=Cloning site Blue=ORF Orange=Stop codon

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCCC

ATGCCCAATTCCGAGAGACATGGGGGCAAGAAGGACGGGAGCGGAGGAGCTTCTGGAACTTCGCAGCCGT
CATCGGGAGGTGGCAGCTCCAACAGCAGGGAGCGTCACCGCTTGGTGTCGAAGCACAAGCGGCATAAGTC
CAAGCACTCCAAAGACGTGGGGCTGGTGACCCCCGAAGCGGCATCTTTGGGTACCATAATCAAACCACTG
GTGGAGTACGATGACATCAGCTCTGATTCAGACACCTTCTCCGATGACACGGCCTTCAAATCAGACCGGA
GGGAGAACGAGGAACGTCGGGGAACGGATCGGAGCGATCGCCTGCACCGACATCGTCACCACCAGCACCG
GCGGTCCCGAGACTTGCTAAAAACTAAACAGACGGAAAAGGAAAAAAATCAGGAAGTCTCCAAATCTGGA
TCTATGAAGGACCGGGTATCGGGCAGTTCCAAACGGTCCGTGGAGGGGAGTGATGATTATGGGAAGGCCC
AGCTATCCAAAAGCGGCAGCAAGGAATCCAGGTCGTCCAAAATGCACAAGGAGAAGACCCGCAAAGAGCG
AGAGTTAAAGTCTGGATACAAGGACCGGAGTAAAAGTCATCGGAAAAGGGAAACACCCAAAAGTTACAAA
ACCGTGGCTAGCCCTAAACGGAGATCCAGGAGTCCCCATAGGAAATGGTCTGACAGTTCCAAGCAAGATG
ACAGCCCTTCCGGAGCTTCTTATGGCCAAGACTACGATCTTAGCCCCCCAAGGTCTCACACTTCTAGCAA
CTATGACTCCTACAAGAAGAGTCCTGGAAGTACCTCAAGAAGGCAGTCAATCAGCCCACCTTACAAAGAG
CCTTCTGCTTACCAGTCCAGCACTCGGTCACCCAGTCCTTACAGCCGACGACAGAGGTCTGTGAGTCCCT
ATAGCCGGAGACGGTCCTCCAGCTATGAAAGGAGCGGCTCTTACAGCGGGAGATCACCCAGCCCCTATGG
CCGAAGGCGATCAAGCAGCCCTTTCCTGAGCAAGAGGTCTCTGAGTCGGAGTCCACTCCCCAGTAGGAAA
TCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACACTCATCTTCTCATAGTAAAAAGAAGCGAT
CCGGGTCACGCAGTCGTCATTCCAGTATCTCACCTGTCAGGCTTCCATTGAATTCCAGCCTGGGAGCTGA
ACTCAGTAGAAAAAAGAAGGAAAGAGCAGCAGCTGCTGCAGCAGCAAAAATGGATGGAAAAGAGTCCAAG
AGTTCACCTATAATTTTGCCTAAAAAAGAGAAACTTGAGGTGAAGGAGTCAGGGTTAGAGTCTAAAAAGT
TACCCAGAAGTATAAAATCAGAAAAATCGACCCCAGATACTGAACTGGTTACTGTAGCACATTCAAACCC
AGAGGTAAAACATTGTTTAGACACAGGGAAGGTTAGGTTGGATGAGAACTTGCAGAAGCATCCTGCTAAG
GATTTGAAAGCACAGGGAACAAAGGACGTTAAACCTGTAGCACCGAAAGAGGTGATTGTTACTTCAAAGG
AGACAGAGACATCAGAAAAGGAGACCCTTCCACCTCTTCCCACAATTACTTCTCCACCCCCTTTACCAGC
TACTACCCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCACTACCAGCTATTCCGCTGCAGCCA
CCTCTGCCTCCTCCCCAACCACCATTTAGTCAAGTTCCTGTTTCAAGTACTTCAATTTTACCCTCTTCTC
CTCACCCAAGGACATCTACTCTATCCTCTCAGACAAATTCTCAGCCCCCTGTACAGGTTTCTATGAAGAC
TCAAGTATCTATAACAGCTGCTATTCCACATCTGAAGACTTCAACATTGCCTCCTCTGCCCCTCCCTCCC
CTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAACACTTCCTTCAAAGCCTGCAAAGAAAGAGA
AGGAACAGAGGACTCGCCACTTGCTTACAGACTTGCCTCTTCCTCCTGAGCTACCAGGAGGAGATCCATC
GCCTCCAGATTCTCCAGAGCCAAAGGCAATTACACCACCTCAACAACCATATAAAAAGAGACCAAAAATT
TGTTGTCCACGTTATGGAGAAAGAAGACAAACAGAAAGTGATTGGGGGAAGCGCTGTGTGGACAAGTTTG
ACATTATTGGGATTATTGGAGAGGGGACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACACAGGAGA
ACTAGTAGCTCTGAAGAAGGTTCGGCTGGACAATGAGAAAGAGGGCTTCCCAATCACAGCCATCCGGGAG
ATCAAGATTCTTCGTCAATTAGTTCACCAGAGTGTTGTAAACATGAAGGAAATTGTCACAGACAAACAAG
ATGCACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAATATATGGACCATGACTTAAT
GGGACTGCTTGAATCAGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCATTTATGAAACAGCTAATG
GAAGGACTGGATTACTGTCACAAAAAGAATTTCCTCCATCGGGATATTAAATGTTCTAACATTTTGCTGA
ATAACAGCGGGCAAATCAAACTGGCAGATTTTGGACTTGCTCGGCTCTATAACTCTGAAGAGAGTCGCCC
TTACACAAACAAAGTCATCACTCTTTGGTATCGACCTCCAGAGCTTCTTCTTGGAGAGGAAAGATACACA
CCAGCCATTGATGTTTGGAGCTGTGGGTGCATCCTTGGAGAACTGTTCACAAAGAAACCTATTTTTCAAG
CCAATTTAGAACTGGCTCAGCTAGAACTTATCAGTCGTCTCTGTGGTAGTCCTTGTCCAGCAGTGTGGCC
TGATGTTATCAAGCTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATACAGGAGACGCCTAAGAGAA
GAATTCTCTTTCATTCCTTCAGCGGCACTTGATCTATTGGACCACATGCTGACACTGGATCCTAGCAAGA
GGTGCACAGCTGAACAGACCCTACAGAGTGACTTTCTTAAAGATGTGGAACTCAGCAAAATGGCACCTCC
AGACCTACCTCACTGGCAGGATTGCCATGAATTGTGGAGTAAGAAACGTCGACGGCAGCGACAGAGTGGT
ATTGTGATAGAAGATCCACCTCCGTCCAAAGCTTCTAGAAAAGAAACTACCTCAGGGACAACAGCTGAGC
CTGTGAAAAACAATAGCCCAGCACCACCTCAGCCTGCTCCTGTCAAGGCAGAGCCTGGTCCAGGGGATGC
AGTAGGCCTTGGTGACATCACACAGCAGTTGAATCAAAGTGAATTGGCAGTGTTATTAAACCTGCTTCAG
AGCCAAACTGACCTGAGCATCCCTCAGATGGCACAGCTGCTTAATATCCACTCCAATCCAGAGATGCAAC
AGCAGCTTGAAGCCTTGAATCAGTCTATTAGTGCACTGACTGAAGCCAGTTCCCAGCAGCAGGACTCAGA
ATCCATAGCCCCTGAAGAATCATTGAAGGAGGTACCTTCTGTACCTGTGGTCCTGCCTCCTGCTGAACAG
ACAACTCCTGAAGCTTCAAACACACCAGCTGACATGCAGAATGTGTTGGCAGTTCTCTTGAGTCAGCTGA
TGAAAACCCAAGAGCCAGCAGGTAACCTGGAGGAAAACACCAATGACAAGAATAGTGGGCCACAGGGGCC
CCGAAGAACTCCTACAATGCCACAGGAGGAGGCAGCAGGTAAACAGACCGGTCATGAATCTCATTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites AscI-MluI |
ACCN NM_026952
Insert Size 3777 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026952.2, NP_081228.2
RefSeq Size 4846 bp
RefSeq ORF 3777 bp
Locus ID 69131
UniProt ID Q14AX6
Cytogenetics 11 61.75 cM
Summary Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026952" in other vectors (4)
SKU Description Size Price
MG219170 Cdk12 (tGFP-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12) transcript variant 3, (10ug) 10 ug
$1,295.00
MR219170 Cdk12 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 3 10 ug
$589.00 $1,095.00
MR219170L3 Lenti ORF clone of Cdk12 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 3 10 ug
$1,395.00
MR219170L4 Lenti ORF clone of Cdk12 (mGFP-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 3 10 ug
$1,395.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.