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Atp2b2 (NM_001036684) Mouse Untagged Clone

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SKU
MC223909
Atp2b2 (untagged) - Mouse ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,670.00
6 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Atp2b2
Synonyms D6Abb2e; dfw; Gena300; jog; PMCA2; Tmy; wms; wri
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223909 representing NM_001036684
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT
TCGGGTGCACCATGGAGGAGCTGCGCTCCCTCATGGAGCTGCGGGGCACCGAGGCTGTGGTCAAGATCAA
GGAGACGTATGGGGACACTGAAGCCATCTGCCGGCGCCTCAAAACCTCGCCTGTTGAAGGTTTACCAGGC
ACTGCTCCAGACTTGGAAAAGAGGAAACAGATTTTTGGGCAAAACTTCATACCTCCAAAGAAACCAAAAA
CCTTCCTGCAGCTGGTGTGGGAAGCGCTACAGGACGTGACACTTATCATCCTGGAGATCGCGGCCATCAT
CTCCCTGGGACTGTCCTTCTACCACCCACCGGGAGAGAGCAATGAAGGATGTGCCACGGCCCAGGGTGGG
GCAGAGGATGAAGGTGAAGCAGAAGCAGGCTGGATTGAGGGGGCTGCCATCCTGCTGTCAGTCATCTGTG
TGGTCCTGGTCACAGCCTTCAATGACTGGAGTAAGGAGAAACAGTTCCGGGGCCTGCAGAGCCGAATTGA
GCAGGAGCAGAAGTTCACTGTGGTCCGGGCCGGCCAGGTGGTTCAGATCCCTGTGGCCGAGATCGTGGTC
GGGGACATTGCCCAGATCAAATATGGTGACCTTCTTCCCGCTGATGGCCTCTTCATCCAGGGAAATGACC
TCAAAATTGATGAAAGCTCACTCACAGGGGAATCTGACCAGGTGCGCAAGTCTGTGGATAAGGACCCCAT
GTTGCTGTCAGGAACCCATGTGATGGAGGGCTCAGGACGGATGGTGGTTACTGCTGTGGGTGTGAACTCT
CAGACTGGCATCATATTTACCCTGCTTGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGCCA
AGCAGCAGGATGGGGCAGCTGCCATGGAGATGCAGCCTCTGAAGAGTGCAGAGGGCGGCGATGCAGATGA
CAAGAAGAAAGCCAACATGCACAAGAAAGAGAAGTCGGTGCTTCAGGGCAAGCTCACCAAACTGGCTGTG
CAGATAGGCAAGGCGGGCCTGGTGATGTCGGCCATCACAGTGATCATCCTGGTACTCTACTTCACCGTGG
ACACCTTCGTGGTCAACAAGAAGCCATGGCTGACGGAATGCACACCCGTCTACGTACAGTACTTTGTCAA
GTTCTTCATCATTGGTGTGACGGTGCTGGTGGTCGCTGTGCCCGAGGGCCTCCCTCTGGCTGTCACCATC
TCACTGGCCTATTCTGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGA
CCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGAACGCTGACCACCAACCGCATGACCGTGGT
CCAGGCCTATGTCGGTGACGTCCACTACAAGGAGATCCCCGATCCCAGCTCCATCAATGCCAAGACGCTG
GAGCTGCTGGTCAACGCCATTGCCATCAACAGCGCCTACACCACCAAGATCCTTCCCCCAGAAAAAGAGG
GAGCCCTGCCCCGGCAGGTGGGCAACAAGACAGAGTGCGGCCTGCTGGGCTTTGTGCTGGACTTGAGGCA
GGACTACGAGCCGGTGCGCAGCCAGATGCCAGAGGAGAAGCTGTATAAGGTGTACACCTTCAACTCCGTG
CGCAAGTCCATGAGCACCGTCATCAAGATGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGCGCCTCGG
AGATTGTGCTCAAAAAGTGCTGCAAGATCCTCAGTGGGGCAGGGGAAGCCCGTGTCTTCCGGCCCCGAGA
CAGGGATGAGATGGTTAAGAAGGTGATTGAGCCCATGGCCTGTGACGGGCTCCGTACCATCTGCGTGGCC
TATCGTGACTTCCCCAGCAGCCCTGAGCCTGACTGGGACAATGAGAATGACATTCTCAATGAACTCACGT
GCATCTGCGTGGTGGGCATCGAAGACCCAGTACGACCTGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCG
GGCAGGTATCACAGTCCGCATGGTCACCGGTGACAATATCAACACAGCCCGGGCCATCGCCATCAAGTGT
GGCATTATCCACCCTGGAGAGGACTTCCTGTGCCTGGAAGGCAAAGAATTCAATCGGAGGATTCGCAACG
AGAAGGGGGAGATTGAGCAGGAGCGGATTGACAAGATCTGGCCAAAGCTGAGGGTGCTGGCTCGCTCCTC
GCCCACGGATAAGCACACGCTGGTCAAAGGCATCATCGACAGTACACACACTGAGCAGCGGCAGGTGGTG
GCTGTGACAGGGGATGGGACCAACGACGGGCCTGCTCTCAAGAAGGCAGATGTGGGCTTCGCAATGGGCA
TCGCAGGCACAGATGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGATGACAACTTCAGCAGCATCGT
CAAGGCAGTGATGTGGGGCCGTAACGTCTATGACAGCATATCCAAATTCCTGCAGTTCCAGCTGACTGTC
AACGTGGTGGCGGTGATCGTGGCCTTCACGGGCGCCTGCATTACACAGGACTCCCCTCTCAAGGCTGTGC
AGATGCTCTGGGTGAACCTCATCATGGACACGTTTGCCTCCCTGGCCCTGGCCACAGAGCCACCTACGGA
GACTCTGCTTCTGAGGAAACCGTACGGTCGCAACAAGCCGCTCATCTCGAGGACCATGATGAAGAACATC
CTGGGCCACGCCGTCTACCAGCTCACCCTCATCTTCACCCTGCTCTTCGTGGGTGAGAAGATGTTCCAGA
TCGACAGCGGAAGGAACGCCCCGCTGCACTCACCACCCTCAGAGCACTACACCATCATCTTCAACACCTT
CGTCATGATGCAGCTTTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAACGTCTTTGACGGG
ATCTTCCGGAACCCCATCTTCTGCACCATCGTTCTTGGCACTTTCGCCATCCAGATAGTGATCGTGCAGT
TTGGCGGGAAGCCCTTCAGCTGCTCCCCACTCCAGCTGGACCAGTGGATGTGGTGCATCTTCATAGGCCT
GGGAGAGCTCGTCTGGGGCCAGGTCATCGCCACCATCCCTACCAGCAGGCTCAAGTTCCTGAAAGAGGCA
GGGCGTCTAACACAGAAGGAGGAGATCCCCGAGGAGGAGTTAAATGAGGATGTGGAAGAGATAGACCACG
CAGAGCGGGAGCTTCGCCGTGGCCAGATCCTGTGGTTCCGGGGCCTGAATCGGATCCAGACACAGATCCG
CGTCGTGAAGGCGTTCCGTAGCTCTCTCTATGAAGGGTTAGAAAAACCCGAGTCTCGAACCTCCATCCAT
AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATCGATGACACCG
ACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCCTCGCTCAACAAGAACAATAGCGC
CATCGACAGCGGGATCAACCTGACGACCGACACGAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCC
ATCCACAGCCTGGAGACGTCGCTTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001036684
Insert Size 3597 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001036684.2, NP_001031761.1
RefSeq Size 6922 bp
RefSeq ORF 3597 bp
Locus ID 11941
UniProt ID Q9R0K7
Cytogenetics 6 52.85 cM
Summary This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 3. These differences result in a shorter protein (isoform 1), compared to isoform 2. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001036684" in other vectors (4)
SKU Description Size Price
MG223661 Atp2b2 (tGFP-tagged) - Mouse ATPase Ca++ transporting plasma membrane 2 (Atp2b2) transcript variant 2, (10ug) 10 ug
$1,764.00
MR223661 Atp2b2 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2), transcript variant 2 10 ug
$1,564.00
MR223661L3 Lenti ORF clone of Atp2b2 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2), transcript variant 2 10 ug
$1,864.00
MR223661L4 Lenti ORF clone of Atp2b2 (mGFP-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2), transcript variant 2 10 ug
$1,864.00

file-alt Citations

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