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Phka1 (NM_173021) Mouse Untagged Clone

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SKU
MC223856
Phka1 (untagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,133.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Phka1
Synonyms 5330411D17; 9830108K24Rik; Phka
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223856 representing NM_173021
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGCCGCAGTAACTCGGGAGTCCGCCTGGACGGCTATGCTCGGCTGGTGCATCAGACCATCCTGT
GCCATCAGAATCCAGTGACAGGCTTGCTTCCAGCCAGCTATGATCAAAAAGATGCCTGGGTCCGAGATAA
TGTGTACAGTATCCTGGCTGTGTGGGGTTTGGGCCTGGCATATCGCAAAAATGCTGACCGTGATGAGGAC
AAGGCAAAGGCCTATGAACTGGAGCAGAGTGTAGTGAAGTTAATGAGGGGACTGCTGCACTGCATGATCA
GACAGGTGGATAAAGTAGAGTCCTTCAAGTACAGTCAGAGTACTAAAGATAGCCTCCATGCCAAGTACAA
CACCAAAACTTGTGCCACTGTGGTGGGTGATGACCAGTGGGGACACCTGCAGTTGGATGCTACTTCTGTG
TACCTGCTCTTCCTAGCACAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGACGAAGTCAATT
TTATACAGAACCTCGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTTGGGATATGGGAACGTGG
CGATAAGACAAACCAAGGCATCTCGGAATTGAATGCGAGTTCAGTTGGAATGGCCAAGGCAGCCCTGGAA
GCACTAGATGAATTAGACTTGTTTGGTGTGAAAGGTGGGCCACAATCAGTTATCCATGTCCTGGCTGATG
AAGTCCAACACTGCCAGTCTATCCTGAATTCACTACTGCCCAGGGCTTCAACATCCAAAGAAGTTGATGC
CAGTCTGCTCTCAGTGGTCTCTTTCCCAGCCTTTGCTGTAGAGGACAGCCATTTGGTGGAGCTCACCAAA
CAGGAGATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGTTTTCTGCGAGATGGATATAAAACTC
CTAAAGAGGATCCCAATCGCCTATACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGCGA
ATGGCCATTGTTCTGGACATACTTTATCCTTGATGGGATCTTCAGTGGCAACGTAGAACAGGTTCAAGAA
TATAGAGAGGCTCTTGATGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCTCTTCTTCCAGAGCTGTACA
GTGTCCCTCCTGACAGGGTTGATGAAGAGTATCAAAATCCCCACACTGTGGATCGAGTCCCTATGGGAAA
ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCTCCT
GGAGAAATTGATCCCCTGAATCGTAGGTTTTCTACTGTGCCAAAGCCAGATGTGGTGGTTCAAGTCTCCA
TTCTGGCTGAAACAGAAGAAATCAAGGCCATTTTGAAGGACAAAGGAATTGATGTGGAGACCATTGCTGA
AGTGTACCCCATAAGAGTACAGCCAGCTCGTATTCTCAGCCATATTTATTCTAGTCTAGGATGCAACAGT
AGAATGAAACTCAGTGGACGACCCTACAGGCTCATGGGTGTGCTTGGAACATCAAAACTTTATGACATTC
GCAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACCA
GATGATAGTAGAAATGCTCAGAACAGACCTTTCCTACCTCTGTAGCCGCTGGAGGATGACAGGCCAGCCC
ACGATCACTTTCCCTATCTCGCACACCATGCTTGATGAAGATGGAACCAGCTTGAATTCAAGTATCTTGG
CAGCACTCCGAAAAATGCAGGATGGCTATTTTGGTGGGGCCAGGATCCAAACAGGTAAATTGTCAGAGTT
TTTGACAACATCTTGCTGCACACACTTAAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA
GATTATGATGAAGACTATGAAGATGATTTGGACTCTGGCAACTGGATGGACAGCTATGATTCAACAAGTA
ATGATGTTCACATGTATCTTCCTACAAAGTTATTTCAGCCTTCTCGTCCTTCACTCAACTTACTTGACTC
CCCTGAGTCTCCACAAGATAGCCAGGTTCCTTCCGTTCATGTAGAAGTGCATCTTCCTAGGGACCAGTCT
GGGGAAGTGGACTTCCAGTCATTGGTTTCACAGTTGAAGGAGACCTCAAGCTTACAGGAGCAAGCTGATA
TACTCTACATGCTGTATTCAATGAAAGGACCTGACTGGAACACTGAATTGTATGAAGAAGGGGGGGCTAC
TGTCAGAGAGCTTCTTAGTGAACTGTATGTCAAAGTTGGTGAAATTCGGCACTGGGGTCTGATCCGATAT
ATCTCTGGGATCTTACGGAAGAAAGTGGAGGCACTTGATGAGGCCTGCACAGACCTTCTGTCCTACCAGA
AACACCTGACAGTAGGACTTCCTCCAGAACCTCGAGAGAAGACCATCTCTGCGCCTCTACCGTACGAGGC
ACTCACTAAGCTGATAGATGAAGCCAGTGAAGGCGACATGAGCATCTCAACCCTTACACAGGAAATAATG
GTCTATCTTGCCATGTATATGAGAACTCAGCCTGGCCTCTTTGCAGAAATGTTCAGACTTCGAATCGGTT
TGATCATACAAGTTATGGCAACAGAACTAGCACACTCTCTTCGATGTTCAGCTGAGGAAGCCACAGAGGG
CCTGATGAATCTCAGTCCTTCAGCCATGAAGAACCTCCTGCATCACATTCTCAGTGGCAAAGAGTTTGGA
GTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTGAGTCCTGCTATTTCCATCCATGAGATTGGTGCTG
TTGGAGCAACAAAAACTGAACGAACTGGAATCATGCAGTTAAAAAGTGAGATAAAGCAGGTGGAATTTCG
TAGGCTGTCTGTCTCGATGGAGAGTCAGACTAGTGGTGGTCATCCCTCGGGTGTAGATTTGATGTCGCCA
TCCTTTCTGTCCCCTGCAGCCTGTATTGCTGCAAGTAGTGGATCCTTTCCTACGGTGTGTGATCATCAGA
CATCTAAAGATAGTCGTCAAGGCCAGTGGCAACGCAGGAGAAGGCTAGATGGAGCACTAAATAGAGTACC
AATTGGATTTTATCAAAAAGTATGGAAAATTTTGCAGAAATGTCATGGGCTTTCTGTGGAAGGTTTTGTT
CTTCCCTCTTCAACCACTAGGGAGATGACCCCAGGTGAGATTAAATTCTCTGTCCATGTGGAGTCTGTCC
TGAATCGTGTCCCTCAGCCAGAATACCGCCAACTTCTGGTTGAAGCCATCCTTGTTCTCACCATGCTGGC
AGATATTGAAATTCATAGCATTGGGAGCATCATTGCTGTGGAGAAAATAGTTCATATTGCCAACGACTTG
TTCCTTCAAGAACAGAAAACCCTCGGCGCAGATGATACCATGTTGGCAAAGGATCCTGCATCTGGCATCT
GTACTCTCCTGTATGACAGTGCACCCAGTGGCAGATTTGGCACCATGACCTACCTCTCCAAGGCAGCTGC
CACCTACGTGCAGGAGTTTCTGCCACACAGCCTCTGTGCCATGCAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_173021
Insert Size 3549 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173021.3, NP_766609.2
RefSeq Size 5938 bp
RefSeq ORF 3549 bp
Locus ID 18679
Cytogenetics X 45.47 cM
Summary Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.
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Documents
Resources
"NM_173021" in other vectors (4)
SKU Description Size Price
MG226481 Phka1 (tGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1) transcript variant 2, (10ug) 10 ug
$1,229.00
MR226481 Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 10 ug
$589.00 $1,029.00
MR226481L3 Lenti ORF clone of Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 10 ug
$1,329.00
MR226481L4 Lenti ORF clone of Phka1 (mGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 10 ug
$1,329.00

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