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Brip1 (NM_178309) Mouse Untagged Clone

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SKU
MC223817
Brip1 (untagged) - Mouse BRCA1 interacting protein C-terminal helicase 1 (Brip1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,023.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Brip1
Synonyms 3110009N10Rik; 8030460J03Rik; BACH; Bach1; FACJ; Fancj; OF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223817 representing NM_178309
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCAGTGTTGTCTGACTACACCATCGGTGGGGTGAAGATCCACTTTCCCTGTAGAGCGTATCCAG
CACAGCTTGCGATGATGAATTCTATTGTCAGAGGACTAAACAGCAGCCAACACTGTTTGCTGGAGAGTCC
CACAGGAAGTGGAAAAAGTTTAGCCTTACTTTGTTCTGCATTAGCATGGCAGCAATCTCTTAGTGAGAAG
CCAGTGGATGAGGGCCTGAATAAAAAGCCTGAGGCACCACCTTCATGTTCTTGTGCATGCCATTCAAAGA
ATTTTACCTACAGCGACACAAACCTGGACACTTCACCTCATTTCAATAGTCCAAGCAAACCATCTTCTGG
AAGAAATGGAGTCTCAACTCCCTGTCAAGACTCTCCTGAAAAAAATACTCTGGCTGCAAAGTTATCTGCC
AAGAAACAGGCATCTATACACAGAGACGAAGATGATGATTTTCAGGTAGAGAAGAAAAGAATCCGACCCT
TAGAAACTACACAGCAGATAAGAAAACGTCATTGTTTGGAAAAGGACGTCCACCATGTAGATGCACGACT
AGCTTCGGAAAAGAGAGTAAAGCCTGAGTCTCCGATAGGAAAGTCTTTCTCCGACAGGAAAGACTCTTTT
CAAAATGTGGATGGCCTTTGTTCCAGGTGCTGCTGTTCTGCTAAACAAGGAAACAACCAAGAGCCTGCAA
ATACTGTTAAGAAAGATCACGGGGGACAATGCAAGAGACCTAAAATTTATTTTGGGACGCGGACACACAA
GCAGATTGCTCAGATTACCAGGGAGCTCCGTAAGACAGCTTACTCAGGGGTCCCGATGACTATTCTTTCC
AGCAGGGACCACAGCTGTGTCCATCCTGAAGTGGTGGGGAATTTCAACAGAAAGGAGAAGTGCATGGAGC
TGCTGGATGGCAAGCACGGGAAGTCCTGCTATTTCTATCATGGTGTTCATAAAATTAGTAATCAGCAGAC
ACTGCAGCACCTTCAGGGCATGTCCAGAGCCTGGGACATAGAAGAACTAGTCAGCCTCGGGAGGAAACTG
AAAGCTTGTCCCTACTATACAGCTCGAGAACTGATAGAGGATGCTGACATAGTGTTTTGTCCCTACAACT
ACCTTTTAGACTCACAAATAAGGGAAACTATGGATATAAAGCTGAAAGGACAAGTTGTCATTTTAGATGA
AGCTCACAACATTGAAGACTGTGCTCGGGAATCAGCAAGCTACAGTGTAACCGAAGTTCAGCTTAGGTTT
GCTCGGGATGAGCTAGATAGTTTGATCAACGGTAACATAAGGAAGAAAAGCCACGAGCCCCTACGAGATG
TGTGCTATAACCTCATCAATTGGTTAGAAACAAATTCTAAGCATCTTGTAGAAAGGGGTTATGAGTCGTC
TTGTAAAATATGGAGTGGAAATGAGATGCTCTTAAACTTATACAGGATGGGCATCACCACGGCTACTTTT
CCAGTTTTGCAGAGGCATCTCTCAGCTGTTCTTCAAAAAGAAGAGAAAGTCACACCAATCCATGGTAAAG
AGGAGGCAATACAAATCCCTATTATCAGTGCATCAACTCAAGTCGTGCTCAAAGGACTCTTTATGGTGCT
TGACTATCTCTTTAGAGAAAATAGCAGATTTGCAGATGATTATAAAGTTGCTATTCAACAGACTTACTCC
TGGACAAATCAGATTGCTATTTTTGATAAAACTGGAGTCTTGGCTGTACCAAAAAATAAGAAACATTCAC
GACAGAAAATTGGAGTTAATGCGCTAAACTTTTGGTGTTTAAATCCAGCTGTGGCCTTTTCAGATATTAA
TGACAAAGTACGGACCATTGTTCTAACATCAGGAACACTCTCACCCCTGAAATCCTTTTCATCTGAGCTT
GGTGTCACATTTAGCATCCAACTGGAGGCTAACCATGTCATCAGTAACTCACAGGTTTGGGTTGGTACTG
TTGGCTCAGGCCCTAAGGGTCGGAATCTCTGCGCTACCTTTCAGCATACTGAAACATTTGAGTTCCAGGA
TGAAGTGGGAATGCTTCTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTCTGTGCTTTTTGCCATCT
TACAAGCTATTAGAGAAATTAAGAGAACGTTGGATTTTCACTGGCCTATGGCATAGTTTGGAGTCAGTGA
AGACAGTCATTGCAGAACCCCAAGGAGGAGAAAAAACTGATTTTGATGAATTGCTGCAAGTGTACTATGA
CGCCATCAAGTTCAAAGGAGAAAAGGATGGAGCCCTTCTCATAGCAGTCTGTCGTGGCAAGGTGAGCGAG
GGTCTGGATTTCTCAGATGATAACGCCCGTGCTGTCATAACCGTAGGGATTCCTTTCCCAAATGTGAAGG
ATCTTCAGGTGGAGCTAAAACGACAATACAATGACCACCACTCAAAATCGAGAGGTCTTCTGCCTGGCCG
TCAGTGGTATGAAATTCAAGCATACCGAGCCTTGAATCAGGCTCTAGGCAGATGTATTCGGCACAAAAAT
GATTGGGGAGCTCTTATTCTAGTGGACGATCGCTTCAATAACAATCCAAACCGCTATATATCAGGACTTT
CTAAATGGGTGAGGCAGCAGATTCAGCACCACTCAAGCTTTGCAAGTGCCCTGGAGTCGCTGACCGAGTT
CTCCAGAAGACACCAGAAAGTTACTAATAGATCCAAAAAAGACGAAAAATGTACAAAAGACAATGAGCCT
ACTCTGGAAGTGGCCTGTTTGGAGGATAGCACTTTTACAAGTGTGTCAGAATCAAGTCATCAGTCACCAG
AAAACTCGACAGAAGAAGCAGAAGTGTGTGTCCAGGAGCTTCAGTGTCCCCAGGTGGCTACTAAAAGCCC
CTCAGTAGCAAGCCACGGTGTTTCCAGAAGGAAGAAAAGTGATCCAGGACTAAGAGGAGAGTCTCTTCAG
ACAATGAAAACAGAAAAAAATGAGATTTCCCGATCTTCAAGCCCAACTTTTGGAAAGCAAACAGAGCCAG
TCAACTGGCCTATCTTTAATTCTCTAAGACGGCATTTCAATAGTAAAGTAAAAAATTGCACACCAGTACT
CAAGTCATCAAAGAACCGTGCCCCTGGTTCTTCCACTTTCAATAAAACTGCTTTGCCACTCACAGGTAAC
TGTGTGCCCTCAAACGAAACAGCAGACACATCACTGGGACCTTGCCTTCAGTCAGAAGTCATAATTTCAC
CAGTGAAGATTGAAGCTACTCCTGCTACGAATTATTCCAAACAGGTGTTCTGCTGTGAGAAAGACCTACT
CCCGGATACTGAATTGTCGCCAGGAACTGAAGAAGCCAAGTGTCCCAGCTCAAACAAAGCTGCTGAAACA
GAAGTGGATGATGACTCTGAGTGTTTTACACCTGAACTTTTTGATCCTGTCGATACCAACGAAGAAAACG
GTGAACTGGTTGAAACTGATAGATCAAGTCATAGTTCAGATTGTTTTTCAGCGGAAGAGCTTTTTGAAAC
TGCAACTGGATTTGGGCAGAAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_178309
Insert Size 3525 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC094252, AAH94252
RefSeq Size 3917 bp
RefSeq ORF 3525 bp
Locus ID 237911
UniProt ID Q5SXJ3
Cytogenetics 11 C
Summary This gene encodes a member of the DEAH subfamily of DEAD box helicases. A similar protein in humans is both a DNA-dependent ATPase and a 5-prime-to-3-prime DNA helicase, and plays a role in the repair of DNA double stranded breaks through interaction with the breast cancer-associated tumor suppressor BRCA1. provided by RefSeq, Feb 2011
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Documents
Resources
"NM_178309" in other vectors (4)
SKU Description Size Price
MG211750 Brip1 (tGFP-tagged) - Mouse BRCA1 interacting protein C-terminal helicase 1 (Brip1) 10 ug
$1,222.00
MR211750 Brip1 (Myc-DDK-tagged) - Mouse BRCA1 interacting protein C-terminal helicase 1 (Brip1) 10 ug
$589.00 $1,022.00
MR211750L3 Lenti ORF clone of Brip1 (Myc-DDK-tagged) - Mouse BRCA1 interacting protein C-terminal helicase 1 (Brip1) 10 ug
$1,322.00
MR211750L4 Lenti ORF clone of Brip1 (mGFP-tagged) - Mouse BRCA1 interacting protein C-terminal helicase 1 (Brip1) 10 ug
$1,322.00

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