Itgae (NM_008399) Mouse Untagged Clone

SKU MC223790

Itgae (untagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Itgae
• Synonyms: A530055J10; alpha-E1; alpha-M290; aM290; CD103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223790 representing NM_008399; Red=Cloning site Blue=ORF; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGTGGCTCTTCCATACCCTTCTCTGCATGGCCAGCCTGAAGCCCCAGGGAGCCTTCAATTTGGATG; TGGACTGGGCCTGGGTCACAGCACTCCAGCCAGGTGCACCGGCTGTGCTCAGCTCCCTTCTGCATCAAGA; CCCCAGCAACAACCAGACCTGCCTCCTGGTAGCCAGACGCAGCTCCAACAGGAATACAGCCGCCCTGTAT; CGATGCGCTATATCCATCAGTCCAGATGAAATTGCTTGCCAGCCAGTGGAGCACATCTGTATGCCAAAGG; GCAGGTACCAGGGAGTGACATTGGTCGGGAACCACAATGGTGTTTTGGTGTGCATTCAGGTACAGGCCCG; GAAGTTCCGCAGCCTCAACTCAGAGCTCACGGGTGCCTGCAGCCTACTCACCCCCAATTTGGACCTCCAG; GCCCAAGCCTACTTCTCTGACCTCGAAGGGTTCCTGGATCCAGGAGCACATGTGGACTCTGGTGACTACT; GTAGAAGCAAAGGAGGCAGCACAGGGGAGGAGAAGAAGTCAGCCAGGAGACGCCGGACAGTGGAGGAGGA; AGACGAGGAGGAAGATGGCACTGAGATCGCTATTGTCCTGGATGGCTCAGGAAGCATTGAACCCTCAGAC; TTCCAGAAGGCCAAAAATTTCATCTCTACCATGATGAGGAACTTCTATGAGAAGTGTTTTGAGTGCAACT; TTGCCCTGGTGCAGTATGGGGCCGTGATCCAGACTGAGTTTGATCTTCAAGAGAGCCGGGACATTAACGC; CTCCCTTGCCAAAGTTCAGAGCATCGTTCAAGTGAAGGAAGTCACTAAGACCGCCTCAGCCATGCAGCAT; GTCCTAGACAATATCTTCATTCCAAGCCGTGGCTCCAGAAAAAAAGCATTGAAGGTCATGGTGGTACTTA; CTGATGGTGACATATTTGGGGACCCTCTCAATCTTACGACTGTCATCAACTCCCCAAAGATGCAGGGTGT; TGTGCGCTTTGCCATTGGGGTGGGTGATGCATTCAAGAATAACAACACTTACAGAGAACTGAAGCTGATT; GCCTCAGACCCCAAAGAGGCCCACACTTTCAAGGTGACCAACTACTCAGCTCTGGATGGGCTGCTGAGCA; AGCTGCAACAACACATCGTTCATATGGAAGGTACAGTTGGAGATGCCCTTCAATATCAACTCGCACAGAC; TGGTTTCAGTGCCCAGATCCTGGACAAGGGGCAGGTGTTGCTTGGCACTGTGGGGGCCTTTAACTGGTCT; GGGGGTGCGCTGCTCTACAGCACACAGAATGGCCGGGGCTGCTTCCTAAACCAGACAGCCAAGGAGGACT; CCAGGACTGTACAGTACAGCTACCTAGGTTACTCCCTTGCGGTGCTACACAAGGCCCACGGCGTCTCTTA; CGTGGCAGGGGCTCCAAGGCACAAGCTCCGTGGGGCTGTGTTTGAGCTCCGGAAGGAGGACAGAGAGGAG; GATGCCTTTGTGCGACGGATAGAAGGAGAGCAGATGGGGTCCTACTTTGGCTCTGTGCTGTGCCCTGTGG; ATATCGACATGGATGGGACCACAGATTTTCTACTGGTGGCTGCTCCATTTTACCACATCCGTGGAGAAGA; AGGCAGAGTCTATGTGTACCAGGTGCCTGAGCAGGATGCTTCCTTCTCCTTGGCACACACACTGAGTGGG; CACCCTGGGCTCACCAATAGCCGCTTTGGCTTTGCCATGGCAGCTGTGGGGGATATCAACCAAGACAAAT; TCACAGATGTGGCCATTGGGGCCCCTCTGGAGGGTTTTGGGGCAGGTGACGGGGCCAGCTATGGCAGCGT; GTACATCTACAACGGACACTCAGGTGGCTTGTATGACAGTCCTTCCCAGCAGATCCGAGCCTCCTCTGTG; GCCTCGGGACTCCACTACTTCGGCATGTCTGTGTCCGGTGGGTTAGATTTCAATGGCGATGGCCTTGCCG; ATATCACAGTTGGCTCTCGGGACTCGGCGGTTGTGCTCCGCTCACGCCCTGTGGTTGACCTGACCGTATC; TATGACCTTTACCCCCGATGCACTGCCCATGGTCTTCATAGGCAAGATGGATGTAAAACTGTGTTTTGAA; GTTGACTCTTCAGGCGTAGCTTCTGAGCCAGGCCTTAGAGAGATGTTCCTCAACTTTACGGTGGATGTGG; ATGTGACCAAGCAAAGGCAGAGGCTGCAGTGTGAGGACTCGAGCGGTTGTCAGAGCTGCCTCAGGAAGTG; GAACGGAGGGTCCTTTCTGTGTGAACACTTCTGGCTCATCTCCACAGAGGAGCTTTGTGAGGAGGACTGC; TTCTCCAACATCACCATCAAGGTCACCTACGAGTTCCAGACGTCTGGGGGAAGGAGGGACTACCCCAACC; CCACCCTGGACCACTACAAGGAACCCTCTGCCATCTTCCAGCTGCCCTACGAGAAGGACTGCAAGAACAA; AGTGTTCTGCATCGCTGAGATCCAGCTGACCACCAACATCTCCCAGCAGGAACTGGTGGTGGGCGTCACA; AAGGAGGTGACCATGAACATCAGCCTGACTAACTCTGGAGAGGATTCCTACATGACAAACATGGCTCTCA; ATTATCCCAGAAACTTACAGTTTAAGAAGATACAAAAGCCAGTATCTCCAGATGTTCAGTGTGATGACCC; CAAGCCAGTTGCTTCTGTCCTGGTCATGAACTGCAAGATTGGTCACCCCATACTCAAGAGATCATCTGTG; AATGTCTCAGTAACTTGGCAGCTAGAGGAGAGTGTCTTTCCAAATAGGACAGCAGATATCACTGTGACCA; TCTCCAATTCCAATGAGAAGTCTTTGGCCAGAGAGACACGCAGCCTTCAATTCAGACATGCCTTCATTGC; AGTTCTTTCCAGACCCTCAGTGATGTACATGAACACAAGCCAAAGCCCTTCTGACCACAAAGAATTCTTC; TTCAATGTGCATGGGGAGAATCTTTTTGGAGCTGTATTCCAGTTGCAAATTTGTGTCCCCATCAAATTAC; AAGATTTCCAGATTGTAAGAGTGAAGAATCTGACAAAGACTCAGGACCACACTGAGTGCACCCAGAGTCA; AGAGCCTGCGTGTGGAAGCGATCCTGTGCAGCATGTAAAAGAATGGCATTCAGTGGTCTGTGCTATCACA; TCTAATAAAGAAAATGTAACCGTGGCCGCAGAGATCTCCGTGGGCCACACTAAGCAGTTACTAAGAGATG; TATCTGAACTGCCGATCCTTGGTGAAATATCTTTCAACAAATCTCTCTATGAGGGGCTGAATGCAGAGAA; CCACAGGACGAAGATCACTGTCATCTTCCTGAAAGAGGAGGAGACACGCTCTTTGCCTCTCATCATTGGC; AGCAGCATCGGAGGCCTCCTGGTCTTGGTTGTGATTATAGCCATTTTGTTCAAGTGTGGCTTTTTCAAAA; GAAAATATCAACAACTGAACTTGGAGAGCACAAGGAGGGCCCAGCTGAAGGCTGACAGTCTGCTCCAAGA; TTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008399
• Insert Size: 3504 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008399.2, NP_032425.2
• RefSeq Size: 3836 bp
• RefSeq ORF: 3504 bp
• Locus ID: 16407
• UniProt ID: Q60677
• Cytogenetics: 11 45.22 cM
• Summary: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).