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Efl1 (NM_175317) Mouse Untagged Clone

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SKU
MC223657
Eftud1 (untagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP tGFP DDK
  $1,080.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Efl1
Synonyms 4932434J20Rik; 6030468D11Rik; AI451340; AU019507; AU022896; D7Ertd791e; Eftud1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223657 representing NM_175317
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTCAGTGGTGTTGATAAGATGATTCGACTTCAGAAGAACACTGCCAACATTAGGAATATCTGTG
TTCTGGCTCATGTTGACCACGGAAAAACTACTCTTGCTGATTGTCTTATATCCAGTAATGGAATCATCTC
CAGCCGCCTGGCAGGCAAGTTAAGGTATATGGACAGCAGAGAAGATGAACAGGTCAGAGGGATCACCATG
AAGTCCAGCGCCATCTCACTACATTATGCAGAAGGTCATGAGGAGTACCTGATTAATCTGATAGACTCTC
CTGGACACGTGGACTTCTCCTCAGAAGTATCAACAGCGGTTCGCATTTGTGATGGATGCATCATTGTGGT
GGATGCTGTGGAGGGGGTCTGTCCACAGACACAGGCAGTTCTTCGACAAGCCTGGCTTGAAAACATCCGC
CCTGTTTTAGTGATCAATAAGATAGACCGCCTGATAGTGGAACTAAAATTCACCCCACAAGAGGCCTATT
CTCACCTCAAGAATATTTTAGAACAGATTAACGCACTTACAGGAACTCTTTTTACTTCTAAAGTCCTAGA
AGAAAGAGCAGAGAGAGAGACAGAATCCCAGGCGAAGCCACATTCTGAGCAAGGAGAGCAAGTGTATGAC
TGGAGCGCGGGCTTGGAGGACGTGGACGACTCGCAGCTGTACTTCTCGCCAGAGCAGGGCAATGTGGTGT
TTACCAGTGCGATAGATGGCTGGGGCTTTGGAATTGAGCACTTTGCCAGAATCTATAGTCAAAAAATTGG
CATCAAGAAGGAAGTTCTTTTGAAAACCTTGTGGGGAGATTATTATATAAATATGAAGGCTAAGAAGATT
ATGAAAGTTGATCAGGCCAAAGGAAAGAAACCTTTATTTGTACAGTTGATCCTGGAAAATATATGGAGTT
TGTATGATGCAGTCTTGAAAAAGGACAAAGAAAAAATTGATAAAATTGTGACTTCTTTAGGATTAAAAAT
TGGAGCCCGGGAGGCACGACATTCAGACCCTAAAGTCCAGATCAATGCCATTTGCAGCCAGTGGCTCCCC
ATATCCCACGCTGTCCTCGCTATGGTGTGTCACAAACTGCCCAGTCCCCTTGACATGACGTCGGAGCGAG
TAGAGAAACTGCTGTGCACAGGATCCCAGACGTTTGAGTCTCTGCCACCGGAGACCCAAGCCCTGAAAGC
AGCTTTTATGAAATGTGGAAGTGAGGATACTGCTCCAGTTATTATCTTTGTTTCCAAAATGTTTGCAGTT
GATGTGAAAGCCTTGCCTCAGAATAAGCCAAGGCCTCTCACTCAAGAAGAAATGGCCCAGAGACGGGAAC
GGGCAAGACAGAGGCACGCAGAGAAGCTTGCAGCAGCCCAGGGGCAAACATCCCAGGGACCCACCCAGGA
TGGAGGTGCCCTTGAAACAAGTCCCCATGAAGATGAGCCAAGAGGTGATGAACCAGATGTGGCAAGTGTA
AGCCGCCAGCCTGTTTCCCAGGAGGAAAGCAGCCAAGAGGCCTTCATCGCATTTGCTCGTGTGTTCAGTG
GCATAGCTCGAAGAGGAAAGAAAATTTTCGTCTTAGGACCCAAGTACAGCCCTGTTGATTTTTTACAGCG
GGTTCCACTAGGCTTCTCAGCTCCACTAGAGGACCTCCCTCCTGTGCCCCACATGGCGTGCTGTACTCTG
GAGAACCTGTACCTTCTGATGGGAAGGGAGCTGGAAGATCTGGAAGAAGTCCCCCCAGGAAACGTGCTGG
GAATTGGAGGCCTCCAGGACTTTGTGCTAAAATCTGCAACTTTGTGCAGCCTGCCGTCCTGCCCACCGTT
TATTCCCCTCAACTTTGAAGCCACCCCGATTGTGAGAGTTGCTGTTGAACCAAAGCACCCAAGTGAAATG
CCTCAGCTTGTGAAAGGAATGAAGCTGCTAAACCAGGCTGACCCCTGTGTCCAGGTTTTAATTCAGGAAA
CAGGAGAGCATGTCCTGGTCACGGCCGGGGAGGTCCACCTTCAGCGATGTTTGGATGACTTACGAGAAAG
GTTTGCAAAGATCCACATCAGTGTATCTGAACCCATTATTCCATTCAGAGAAACGATCACAAAACCTCCC
AAAGTTGACATGGTCAATGAAGAAATAGGCAGGCAGCAAAAAGTTGCTGTCATACACCAAACAAAAGAAG
AGCAAAGCAAAATCCCAGAGGGGATCCACGTGGACTCGGATGGGCTTATCACCATACCGACTCCCAACAA
ACTGGCCACTCTCAGTGTCCGTGCCATACCGCTTCCAGAAGAAGTCACCAGGATTCTGGAAGAAAACAGC
GACTTGATTCGTTCCATGGAGCTTTTGACATCCTCCTTGAACGAGGGTAGAAATACCCAAGCCATTCATC
AGAAGACCCAAGAGAAAATATGGGAATTCAAAGGAAAGCTAGAGAAACATCTAACAGGGAGGAAATGGAG
GAACACTGTTGACCAGATCTGGTCATTTGGCCCAAGGAAATGTGGGCCCAACATCCTAGTCAGTAGAAGT
GAAGATTTCCAGAACTCAGTATGGTCTGGCCCAGCTGGCAGAGAATCAAAAGAGGCCAGCAGATTCCGAG
ACTTTGGCAATAGCATTGTGAGTGGCTTCCAGCTTGCGACCCTCTCTGGCCCCATGTGTGAGGAGCCGCT
CATGGGTGTCTGTTTTGTTCTAGAAAAGTGGGAGCTAAATAAATGTGCAGAACAAGGAGCAAGTGATAAA
CAGCACCAAGGTCAGTGTGATCTGGCAGGAGAGGGACAGGGGGGAGGTAAAACCTGTCACGTTGGAGATG
AAAACCAAGAGCAACAAGATGTCTGCTCGGAGCCCTTTGAAGAGACTTCCCAGAAAGGAGACTCTCCGGT
TATTGACTGCTATGGACCCTTCTCCGGGCAGCTAATTGCTACCATGAAAGAAGCCTGCCGCTACGCTCTG
CAGGTGAAACCTCAACGCCTGATGGCAGCTATGTACACATGTGACATCATGGCCACCAGTGATGTCCTCG
GCCGAGTCTATGCCGTGCTGTCAAAGCGAGAAGGTCGAGTGCTTCAGGAAGAGATGAAGGAAGGGACGGA
CATGTTCATCATCAAGGCTGTGCTGCCTGTAGCGGAAAGCTTTGGCTTTGCGGATGAGATCAGGAAGAGG
ACCAGCGGACTGGCCAGCCCACAGCTGGTGTTCAGCCACTGGGAGGTCATTCCCAGCGACCCGTTCTGGG
TGCCGACCACGGAGGAGGAGTACCTGCACTTTGGGGAGAAGGCGGATTCCGAGAACCAGGCCCGGAAGTA
CATGAATGCCGTGCGGAAGCGGAAGGGCCTCTATGTGGAAGAAAAGATTGTGGAACACGCAGAAAAGCAG
AGAACCCTCAGCAAAAACAAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_175317
Insert Size 3384 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175317.3, NP_780526.2
RefSeq Size 3637 bp
RefSeq ORF 3384 bp
Locus ID 101592
UniProt ID Q8C0D5
Cytogenetics 7 47.25 cM
Summary Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_175317" in other vectors (5)
SKU Description Size Price
MG211672 Eftud1 (tGFP-tagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1) 10 ug
$1,181.00
MG216621 Eftud1 (tGFP-tagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1) transcript variant 1, (10ug) 10 ug
$1,181.00
MR216621 Eftud1 (Myc-DDK-tagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1), transcript variant 1 10 ug
$589.00 $981.00
MR216621L3 Lenti ORF clone of Eftud1 (Myc-DDK-tagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1), transcript variant 1 10 ug
$1,281.00
MR216621L4 Lenti ORF clone of Eftud1 (mGFP-tagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1), transcript variant 1 10 ug
$1,281.00

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