Cd163 (NM_053094) Mouse Untagged Clone

SKU MC223654

Cd163 (untagged) - Mouse CD163 antigen (Cd163), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Cd163
• Synonyms: CD163v2; CD163v3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223654 representing NM_053094; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGTGGACACAGAATGGTTCTTCTTGGAGGTGCTGGATCTCCTGGTTGTAAAAGGTTTGTCCATCTAG; GTTTCTTTGTTGTGGCTGTGAGCTCACTTCTCAGTGCCTCTGCTGTCACTAACGCTCCTGGAGAAATGAA; GAAGGAACTGAGACTGGCGGGTGGTGAAAACAACTGTAGTGGGAGAGTGGAACTTAAGATCCATGACAAG; TGGGGCACAGTGTGCAGTAACGGCTGGAGCATGAATGAAGTGTCCGTGGTTTGCCAGCAGCTGGGATGCC; CAACTTCTATTAAAGCCCTTGGATGGGCTAACTCCAGCGCCGGCTCTGGATATATCTGGATGGACAAAGT; TTCTTGTACAGGGAATGAGTCAGCTCTTTGGGACTGCAAACATGATGGGTGGGGAAAGCATAACTGTACC; CATGAAAAAGATGCTGGAGTGACCTGCTCAGATGGATCTAATTTGGAGATGAGACTGGTGAACAGTGCGG; GCCACCGATGCTTAGGAAGAGTAGAAATAAAGTTCCAGGGAAAGTGGGGGACGGTGTGTGACGACAACTT; CAGCAAAGATCACGCTTCTGTGATTTGTAAACAGCTTGGATGTGGAAGTGCCATTAGTTTCTCTGGCTCA; GCTAAATTGGGAGCTGGTTCTGGACCAATCTGGCTCGATGACCTGGCATGCAATGGAAATGAGTCAGCTC; TCTGGGACTGCAAACACCGGGGATGGGGCAAGCATAACTGTGACCATGCTGAGGATGTCGGTGTGATTTG; CTTAGAGGGAGCAGATCTGAGCCTGAGACTAGTGGATGGAGTGTCCAGATGTTCAGGAAGATTGGAAGTG; AGATTCCAAGGAGAATGGGGGACCGTGTGTGATGATAACTGGGATCTCCGGGATGCTTCTGTGGTGTGCA; AGCAACTGGGATGTCCAACTGCCATCAGTGCCATTGGTCGAGTTAATGCCAGTGAGGGATCTGGACAGAT; TTGGCTTGACAACATTTCATGCGAAGGACATGAGGCAACTCTTTGGGAGTGTAAACACCAAGAGTGGGGA; AAGCATTACTGTCATCATAGAGAAGACGCTGGTGTGACATGTTCTGATGGAGCAGATCTGGAACTTAGAC; TTGTAGGTGGAGGCAGTCGCTGTGCTGGCATTGTGGAGGTGGAGATTCAGAAGCTGACTGGGAAGATGTG; TAGCCGAGGCTGGACACTGGCAGATGCGGATGTGGTTTGCAGACAGCTTGGATGTGGATCTGCGCTTCAA; ACCCAGGCTAAGATCTACTCTAAAACTGGGGCAACAAATACGTGGCTCTTTCCTGGATCTTGTAATGGAA; ATGAAACTACTTTTTGGCAATGCAAAAACTGGCAGTGGGGCGGCCTTTCCTGTGATAATTTCGAAGAAGC; CAAAGTTACCTGCTCAGGCCACAGGGAACCCAGACTGGTTGGAGGAGAAATCCCATGCTCTGGTCGTGTG; GAAGTGAAACACGGAGACGTGTGGGGCTCCGTCTGTGATTTTGACTTGTCTCTGGAAGCTGCCAGTGTGG; TGTGCAGGGAATTACAATGTGGAACAGTCGTCTCTATCCTAGGGGGAGCACATTTTGGAGAAGGAAGTGG; ACAGATCTGGGGTGAAGAATTCCAGTGTAGTGGGGATGAGTCCCATCTTTCACTATGCTCAGTGGCGCCC; CCGCTAGACAGAACTTGTACCCACAGCAGGGATGTCAGCGTAGTCTGCTCACGATACATAGATATTCGTC; TGGCAGGCGGCGAGTCCTCCTGTGAGGGAAGAGTGGAGCTCAAGACACTCGGAGCCTGGGGTCCCCTCTG; CAGTTCTCATTGGGACATGGAAGATGCTCATGTCTTATGTCAGCAGCTGAAGTGTGGGGTTGCCCAATCT; ATTCCAGAAGGAGCACATTTTGGGAAAGGAGCTGGTCAGGTCTGGAGTCACATGTTCCACTGCACTGGAA; CTGAGGAACATATAGGAGATTGCCTCATGACTGCTCTGGGTGCGCCGACGTGTTCCGAAGGACAGGTGGC; CTCTGTCATCTGCTCAGGAAACCAATCCCAGACACTATTGCCATGTAGTTCATTGTCTCCAGTCCAAACA; ACAAGCTCTACAATTCCAAAGGAGAGTGAAGTTCCCTGCATAGCAAGTGGCCAGCTTCGCTTGGTAGGTG; GAGGTGGTCGCTGCGCTGGAAGAGTGGAGGTCTACCACGAGGGCTCTTGGGGCACCGTCTGTGATGACAA; TTGGGATATGACTGATGCCAATGTGGTGTGCAAGCAGCTGGACTGTGGCGTGGCAATTAACGCCACTGGC; TCTGCTTACTTCGGGGAAGGAGCAGGAGCTATCTGGCTAGACGAAGTCATCTGCACTGGGAAAGAGTCTC; ATATTTGGCAGTGCCATTCACATGGCTGGGGACGCCATAACTGCAGGCACAAAGAAGATGCAGGTGTTAT; CTGCTCCGAGTTCATGTCTCTGAGGCTGACCAACGAAGCCCACAAAGAAAACTGCACAGGTCGCCTTGAA; GTGTTTTACAATGGTACATGGGGCAGTATTGGCAGTAGCAATATGTCTCCAACCACTGTGGGGGTGGTGT; GCCGTCAGCTGGGCTGTGCAGACAACGGGACTGTGAAACCCATACCTTCAGACAAGACACCATCCAGGCC; CATGTGGGTAGATCGTGTGCAGTGTCCAAAAGGAGTTGACACTTTGTGGCAGTGCCCCTCGTCACCTTGG; AAACAGAGACAGGCCAGCCCCTCCTCCCAGGAGTCCTGGATCATCTGTGACAACAAAATAAGACTCCAGG; AAGGGCATACAGACTGTTCTGGACGTGTGGAGATCTGGCACAAAGGTTCCTGGGGAACAGTGTGTGATGA; CTCCTGGGATCTTAATGATGCTAAGGTTGTATGTAAGCAGTTGGGCTGTGGCCAAGCTGTGAAGGCACTA; AAAGAAGCAGCATTTGGTCCAGGAACTGGGCCCATATGGCTCAATGAAATTAAGTGTAGAGGGAATGAGT; CTTCCCTGTGGGATTGTCCTGCCAAACCGTGGAGTCACAGCGACTGTGGGCACAAAGAAGATGCTTCCAT; CCAGTGCCTCCCAAAAATGACTTCAGAATCACATCATGGCACAGGTCACCCCACCCTCACGGCACTCTTG; GTTTGTGGAGCCATTCTATTGGTCCTCCTCATTGTCTTCCTCCTGTGGACTCTGAAGCGACGACAGATTC; AGCGACTTACAGTTTCCTCAAGAGGAGAGGTCTTGATACATCAAGTTCAGTACCAAGAGATGGATTCAAA; GGCGGATGATCTGGACTTGCTGAAATCCTCGGGGGTCATTCAGAGGCACACTGAGAAGGAAAATGATAAT; TTATAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053094
• Insert Size: 3366 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_053094.2, NP_444324.2
• RefSeq Size: 4409 bp
• RefSeq ORF: 3366 bp
• Locus ID: 93671
• UniProt ID: Q2VLH6
• Cytogenetics: 6 F2
• Summary: Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.