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Nckap1 (NM_016965) Mouse Untagged Clone

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SKU
MC223648
Nckap1 (untagged) - Mouse NCK-associated protein 1 (Nckap1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,081.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nckap1
Synonyms C79304; H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1; p125Nap1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223648 representing NM_016965
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGCTCCGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG
GCGTCGGCATGCTCACGCGCCTCTACAACATCAAGAAGGCATGTGGCGACCCCAAGGCTAAACCCTCCTA
CCTTATTGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTTCCTGCTGTAGAAACGCGC
AACAACAATCAACAGCTGGCACAGCTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCGTTGTATT
ACTTTACATTTGTAGATGTTATGGAATTTAAGGACCATGTCTGTGACTTGCTGAATACTATTGACGTTTG
CCAAGTCTTCTTTGATATTACTGTGAACTTTGATTTAACAAAGAACTACTTAGACTTGACTGTAACCTAT
ACAACATTAATGATACTGCTGTCCCGGATTGAAGAACGGAAGGCAATCATTGGACTATACAATTATGCAC
ATGAAATGACCCATGGAGCAAGTGACCGAGAATATCCACGCCTTGGTCAGATGATTGTGGACTATGAAAA
CCCTTTAAAAAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCGCTAATTTCTCTT
CAGATGGTGTATCCTAGACGGAACCTTTCAGCAGACCAGTGGAGGAATGCCCAGTTGCTGAGTCTCATCA
GTGCACCCAGCACAATGCTTAATCCTGCGCAGTCGGACACTATGCCTTGTGAATATCTCTCCTTGGATGC
AATGGAGAAATGGATCATCTTTGGCTTTATTCTGTGCCATGGGATGCTAAATACGGAGGCTACCGCACTG
AACCTTTGGAAGCTAGCTCTTCAGAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTTTTCCACATTC
ACAAAGCTGCAGAAGACTTATTTGTAAACATTCGGGGCTACAATAAACGTATTAACGACATAAGAGAATG
CAAGGAGGCGGCTGTATCCCACGCCGGCTCAATGCACAGAGAAAGACGCAAGTTTTTACGGTCTGCACTA
AAAGAACTGGCCACTGTCCTCTCTGACCAGCCTGGCCTGCTTGGTCCCAAGGCACTTTTTGTTTTCATGG
CGTTATCTTTTGCCCGTGATGAAATTATCTGGCTGCTCCGTCACGCGGACAACATGCCAAAGAAGAGTGC
AGATGACTTTATAGATAAGCACATTGCTGAGCTAATATTTTACATGGAAGAGCTTAGAGCACATGTCAGG
AAGTATGGGCCTGTCATGCAGAGGTACTACGTGCAATACCTCTCTGGCTTTGATGCTGTTGTCCTCAATG
AACTTGTGCAGAACCTGTCTGTTTGCCCTGAGGATGAATCGATCATCATGTCCTCTTTTGTGAACACCAT
GACCTCCCTAAGTGTGAAGCAAGTTGAAGATGGAGAAGTGTTTGATTTCAGAGGGATGAGATTAGATTGG
TTTAGGTTACAGGCGTACACTAGCGTCTCCAAAGCTTCACTTAGCCTTGCAGATCACAGAGAACTTGGAA
AGATGATGAACACAATCATATTCCATACAAAAATGGTAGATTCCTTGGTTGAAATGTTGGTGGAAACATC
CGATCTCTCCATATTTTGTTTTTACAGCCGTGCTTTTGAGAAGATGTTTCAGCAGTGCTTGGAGCTGCCC
TCTCAGTCAAGATACTCCATTGCCTTTCCTCTGCTCTGCACTCACTTCATGAGCTGCACACATGAACTGT
GTCCTGAAGAGCGCCATCATATAGGAGATCGTAGCCTTTCCCTGTGCAATATGTTCCTGGATGAGATGGC
CAAACAAGCTCGAAATCTCATCACCGATATTTGCACAGAACAGTGTACTCTTAGTGACCAGTTATTGCCG
AAGCATTGTGCCAAAACCATCAGTCAAGCAGTGAATAAGAAGTCAAAAAAACAGACGGGCAAGAAAGGGG
AACCTGAAAGGGAAAAACCGGGTGTGGAGAGCATGAGGAAAAACAGGCTGGTAGTGACCAACCTTGATAA
GTTGCACACTGCACTTTCGGAGTTATGCTTCTCCATAAATTATGTTCCAAACATGGCGGTGTGGGAGCAC
ACCTTCACGCCGAGGGAGTATTTGACTTCTCATCTGGAAATCCGGTTCACTAAATCAATTGTTGGAATGA
CTATGTATAATCAAGCTACACAGGAAATTGCAAAGCCATCAGAGCTTCTAACAAGCGTAAGAGCATATAT
GACTGTACTCCAGTCCATAGAGAACTATGTGCAGATTGATATCACCAGAGTATTTAATAATGTCCTTCTT
CAGCAAACACAACACTTAGACAGCCATGGAGAACCAACCATCACGAGTCTGTATACAAACTGGTACTTGG
AAACTTTATTAAGACAAGTCAGCAATGGCCATATAGCTTATTTTCCTGCAATGAAAGCATTTGTGAATTT
ACCCACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGATATATCAGAAATGAGATCATTATCA
GAGCTCCTAGGCCCATATGGGATGAAGTTCCTGAGTGAGAGCCTTATGTGGCACATTTCATCACAAGTAG
CTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTTCTAACACAAATGAGGACCAGCTTTGACAAACC
AGACCAGATGGCTGCACTCTTTAAAAGATTATCATCTGTTGACAGTGTCTTGAAAAGGATGACGATAATT
GGTGTTATTTTATCCTTCCGGTCATTGGCACAAGAAGCATTGAGAGATGTCCTGTCCTACCACATTCCTT
TCCTCGTGAGCTCCATTGAAGATTTCAAGGATCACATTCCTCGGGAAACTGATATGAAGGTTGCAATGAA
TGTGTATGAGCTGTCCTCGGCTGCTGGGTTACCATGTGAGATTGATCCTGCCTTAGTCGTAGCTCTTTCC
TCACAAAAATCAGAGAACATTAGTCCAGAGGAAGAATACAAGATCGCTTGTCTCCTGATGGTCTTTGTAG
CAGTTTCCTTGCCTACACTGGCCAGCAATGTAATGTCACAGTACAGCCCTGCAATAGAAGGACACTGCAA
CAATATACATTGTTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGT
ATTGAAGACCGACTTAAGGAATTCCTGGCGCTTGCATCATCCAGTCTACTGAAAATTGGCCAAGAGACAG
ATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTGCTACTGGATATGATTGTACAAGAATCACCATT
CCTGACAATGGATCTCTTGGAATCTTGTTTTCCTTATGTGTTGCTGAGAAATGCATACCATGCTGTCTAC
AAACAAAGTGTTACATCTTCTGCATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_016965
Insert Size 3387 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016965.3, NP_058661.1
RefSeq Size 4403 bp
RefSeq ORF 3387 bp
Locus ID 50884
UniProt ID P28660
Cytogenetics 2 48.21 cM
Summary Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' end compared to variant 1, resulting in an isoform (b) that is shorter compared to isoform a.
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Documents
Resources
"NM_016965" in other vectors (4)
SKU Description Size Price
MG217978 Nckap1 (tGFP-tagged) - Mouse NCK-associated protein 1 (Nckap1), (10ug) 10 ug
$1,673.00
MR217978 Nckap1 (Myc-DDK-tagged) - Mouse NCK-associated protein 1 (Nckap1) 10 ug
$1,473.00
MR217978L3 Lenti ORF clone of Nckap1 (Myc-DDK-tagged) - Mouse NCK-associated protein 1 (Nckap1) 10 ug
$1,773.00
MR217978L4 Lenti ORF clone of Nckap1 (mGFP-tagged) - Mouse NCK-associated protein 1 (Nckap1) 10 ug
$1,773.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.