Grin3a (NM_001033351) Mouse Untagged Clone

SKU MC223627

Grin3a (untagged) - Mouse glutamate receptor ionotropic, NMDA3A (Grin3a), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Grin3a
• Synonyms: 6430537F04; A830097C19Rik; mKIAA1973; NMDAR-L; NR3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223627 representing NM_001033351; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGAGACTGAGTTTGTGGTGGCTGCTGAGCAGGGTCTGTCTGTTGCTGCCTCCGCCCTGTGCACTGG; TGCTGGCCGGGGTGCCCAGCTCCTCCTCGCACCCGCAACCCTGCCAGATCCTCAAGCGCATCGGACACGC; GGTGAGGGTGGGCGCGGTGCACTTGCAACCCTGGACCACGGCCCCACGCGCAGCCAGTCGCGCTCAGGAC; GGCGGCAGGGCGGGTGCCCAGAGGGATGAGCCAGAGTCGGGGACGTGGCGGCCACCGGCGCCCTCGCAAG; GCGCACGTTGGTTGGGGAGCGCCCTGCATGGCCGGGGTCCACCCGGCTCCCGCAAGCTCGGGGAGGGCGC; CGGGACCGAGACCCTGTGGCCGCGGGATGCCCTACTGTTCGCTGTGGAAAACCTGAACCGTGTGGAAGGG; CTCCTTCCCTACAACCTGTCTTTGGAAGTAGTGATGGCCATCGAGGCGGGCCTGGGCGACCTGCCACTTA; TGCCTTTCTCTTCCCCAAGCTCACCGTGGAGCAGTGACCCTTTCTCCTTTCTGCAGAGCGTGTGCCACAC; CGTAGTGGTACAAGGGGTTTCAGCGCTGCTGGCCTTCCCCCAGAGCCAGGGCGAAATGATGGAGCTGGAC; TTGGTCAGTTCTGTCCTGCACATCCCAGTGCTCAGCATAGTGCGCCACGAGTTTCCGCGGGAGAGTCAGA; ATCCCCTGCACCTACAGCTGAGTTTAGAAAATTCACTAAGTTCTGATGCTGATGTCACTGTCTCAATCCT; GACCATGAACAACTGGTACAATTTTAGCTTGTTGCTATGCCAGGAAGACTGGAATATCACCGACTTCCTA; CTCCTTACAGAGAATAACTCCAAGTTCCACCTCGAGTCTATCATCAACATCACTGCTAACCTGTCCTCCA; CAAAGGACCTTCTAAGTTTCCTGCAGGTCCAGCTGGAAAACATTAGGAACAGCACACCCACAATGGTGAT; GTTTGGCTGTGACATGGGGAGCATCCGGCAGATATTTGAAATGTCCACACAGTTTGGGCTATCACCTCCT; GATCTTCACTGGGTTTTAGGAGACTCACAGAATGTGGAGGAGCTGAGGACAGAAGGCCTGCCCTTAGGGC; TCATTGCTCATGGAAAAACCACACAGTCTGTCTTTGAGTACTATGTTCAGGATGCCATGGAGTTGGTTGC; AAGAGCTGTAGCCACAGCCACCATGATCCAGCCAGAACTTGCTCTCCTTCCCAGCACAATGAACTGCATG; GATGTGAAAACCACAAATCTCACTTCTGGACAATATTTATCAAGGTTTTTAGCCAACACCACTTTCAGAG; GTCTCAGTGGTTCCATCAAAGTAAAGGGATCCACCATCGTCAGCTCAGAAAACAACTTTTTCATCTGGAA; CTTGCAGTATGACCCTATGGGAAAGCCAATGTGGACTCGCCTGGGTAGCTGGCAAGGGGGAAGGATTGTC; ATGGACTCGGGAATATGGCCAGAGCAGGCCCAGAGGCACAAAACCCACTTCCATCACCCAAACAAGTTAC; ACTTGAGAGTGGTGACACTGATTGAACATCCATTTGTTTTCACGAGAGAAGTAGATGATGAAGGCTTATG; CCCTGCTGGACAACTCTGTCTAGACCCTATGACTAATGACTCTTCCATACTGGATAGCCTGTTTAGCAGC; CTACATAGCAGTAATGATACAGTGCCAATCAAGTTCAAGAAGTGCTGCTATGGGTATTGCATTGATCTAC; TGGAACAGTTAGCAGAAGACATGAACTTTGACTTTGACCTCTATATTGTAGGGGATGGAAAGTATGGAGC; TTGGAAAAATGGTCACTGGACTGGGCTGGTTGGTGATCTCCTGAGTGGAACAGCCAACATGGCAGTCACT; TCTTTCAGCATCAATACTGCAAGAAGCCAAGTGATAGATTTCACCAGCCCTTTCTTCTCAACCAGTTTGG; GCATCTTAGTGAGGACTCGAGACACAGCAGCTCCCATTGGAGCCTTCATGTGGCCACTCCACTGGACCAT; GTGGCTGGGGATTTTCGTGGCTCTACATATCACTGCCATCTTCCTCACTCTGTATGAATGGAAGAGCCCC; TTTGGTATGACTCCTAAAGGGAGGAACAGAAACAAAGTCTTCTCCTTCTCCTCAGCCTTGAATGTCTGCT; ATGCCCTTCTGTTTGGCAGAACAGCAGCCATCAAACCCCCTAAATGCTGGACTGGAAGATTTCTGATGAA; TCTTTGGGCCATTTTCTGTATGTTTTGCCTTTCTACATACACAGCGAACTTGGCTGCTGTCATGGTAGGT; GAGAAGATCTATGAAGAACTTTCTGGAATCCATGACCCTAAGCTTCATCATCCTTCTCAAGGTTTTCGTT; TTGGAACTGTCCGGGAAAGCAGTGCTGAGGACTATGTACGGCAGAGCTTCCCAGAGATGCATGAGTACAT; GAGAAGGTACAATGTACCAGCCACCCCTGATGGAGTGCAGTACCTGAAGAATGATCCAGAGAAACTAGAT; GCCTTCATCATGGACAAAGCCCTTCTGGATTATGAAGTGTCAATAGATGCTGACTGCAAGCTTCTGACCG; TAGGAAAGCCATTTGCCATTGAAGGCTATGGCATTGGTCTCCCTCCCAACTCTCCGTTGACCTCTAACAT; ATCTGAGCTCATCAGTCAGTACAAGTCTCATGGGTTTATGGATGTGCTTCATGACAAGTGGTACAAGGTG; GTTCCCTGCGGAAAGAGAAGCTTTGCTGTCACTGAGACTTTGCAAATGGGCATCAAGCATTTCTCTGGAC; TCTTCGTGCTGCTGTGCATAGGATTCGGTCTCTCCATCCTGACAACCATTGGTGAACACATAGTGTACAG; ACTGCTGCTACCACGAATCAAAAACAAATCCAAGCTGCAGTACTGGCTGCACACCAGTCAGAGGTTTCAC; AGAGCATTAAACACATCATTTGTAGAAGAAAAGCAGCCATGTTCCAAGACAAAACGTGTGGAAAAGAGGT; CCAACATGGGACCCCAGCAGCTCATGGTATGGAATACTTCCAATCTGAGTCACGACAACCAACGAAAATA; CATCTTTAATGATGAGGAAGGACAAAACCAGCTGGGTACCCAGACCCATCAGGACATCCCTCTCCCTCCA; AGGAGAAGAGAGCTCCCTGCTTCACTGACCACCAATGGGAAAGCAGACTCCCTCAATGTAGCTCGGAACT; CCGTGATGCAGGAACTCTCCGAGTTGGAGAAGCAGATCCAAGTGATCCGCCAGGAGCTGCAGTTGGCCGT; AAGCAGGAAGACAGAGCTTGAGGAGTATCAAAGGACGAACCGGACTTGTGAATCCTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033351
• Insert Size: 3348 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001033351.2, NP_001028523.1
• RefSeq Size: 7667 bp
• RefSeq ORF: 3348 bp
• Locus ID: 242443
• UniProt ID: A2AIR4
• Cytogenetics: 4 B1
• Summary: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. During the development of neural circuits, plays a role in the synaptic refinement period, restricting spine maturation and growth (By similarity). By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed:24297929). May also play a role in PPP2CB-NMDAR mediated signaling mechanism (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.