Pold1 (NM_011131) Mouse Untagged Clone
SKU
MC223588
Pold1 (untagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1), (10ug)
$1,059.00
6 Weeks*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Pold1 |
| Synonyms | 125kDa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_011131 |
| Insert Size | 3318 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011131.3, NP_035261.3 |
| RefSeq Size | 3428 bp |
| RefSeq ORF | 3318 bp |
| Locus ID | 18971 |
| UniProt ID | P52431 |
| Cytogenetics | 7 28.83 cM |
| Summary | As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites.UniProtKB/Swiss-Prot Function |
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Resources
"NM_011131" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG217545 | Pold1 (tGFP-tagged) - Mouse polymerase (DNA directed) delta 1 catalytic subunit (Pold1), (10ug) | 10 ug |
$1,163.00
|
|
| MR217545 | Pold1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1) | 10 ug |
$589.00
$963.00
|
|
| MR217545L3 | Lenti ORF clone of Pold1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1) | 10 ug |
$1,263.00
|
|
| MR217545L4 | Lenti ORF clone of Pold1 (mGFP-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1) | 10 ug |
$1,263.00
|
|
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.