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Atp9a (NM_015731) Mouse Untagged Clone

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SKU
MC223345
Atp9a (untagged) - Mouse ATPase, class II, type 9A (Atp9a), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,003.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Atp9a
Synonyms IIa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223345 representing NM_015731
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGACAGCATCCCGCTGCAGCCCGTGCGCCACAAGAAGCGGGTGGACAGTAGGCCGCGCGCGGGGT
GCTGTGAGTGGCTGAGATGTTGCGGTGGAGGGGAGCCCAGGCCCCGTACTGTCTGGTTGGGACACCCCGA
GAAGAGGGACCAGCGGTACCCTCGAAATGTCATCAACAACCAGAAGTACAATTTCTTCACATTTCTTCCT
GGGGTGTTGTTCAGCCAGTTCAGATACTTCTTCAACTTCTACTTCCTGCTTCTCGCCTGCTCGCAGTTCG
TCCCAGAGATGAGGCTTGGCGCCCTGTACACCTACTGGGTTCCTCTGGGCTTCGTGCTGGCTGTCACCAT
CATCCGTGAGGCAGTAGAGGAGATCCGATGTTATGTGCGTGACAAGGAGATGAACTCCCAGGTCTACAGC
CGGCTCACGTCACGAGGGACCGTGAAGGTGAAGAGTTCAAACATCCAGGTGGGAGACCTCATCCTTGTGG
AAAAGAACCAGCGGGTCCCTGCTGACATGATCTTCCTGAGGACGTCAGAGAAAAACGGCTCTTGCTTCTT
GCGCACGGATCAGCTGGATGGAGAGACAGACTGGAAGCTTCGGCTCCCGGTGGCCTGCACACAGAGGCTT
CCCACGGCTGCTGACCTCCTGCAGATTCGGTCCTATGTGTACGCTGAAGAGCCCAACATCGACATTCACA
ACTTCCTGGGGACTTTCACCAGGGAAGACAGTGACCCTCCGATCAGTGAGAGTCTGAGCATTGAGAACAC
GCTGTGGGCCGGCACCGTCATAGCATCAGGCACTGTTGTAGGCGTTGTTCTCTACACTGGCAGAGAACTG
CGGAGTGTCATGAATACTTCCGACCCCAGAAGTAAGATTGGCCTGTTCGACCTGGAGGTGAACTGCCTCA
CCAAGATCCTGTTTGGTGCGCTGGTGGTGGTGTCGCTGGTCATGGTGGCCCTGCAGCACTTTGCCGGCCG
CTGGTACCTGCAGATCATCCGCTTCCTGCTCCTGTTTTCCAACATCATTCCTATCAGCTTGCGTGTGAAC
TTGGACATGGGCAAGATCGTGTACAGCTGGGTGATCCGCAGGGATTCCAAAATCCCCGGGACCGTGGTTC
GTTCCAGCACAATTCCTGAGCAGCTGGGCAGGATTTCGTACTTGCTCACAGACAAGACAGGAACCCTGAC
CCAGAATGAGATGGTGTTCAAGCGGCTGCACCTGGGTACGGTGGCCTACGGCCTGGACTCCATGGACGAA
GTGCAGAGTCACATCTTCAGCATTTACACCCAGCAATCCCAGGATCCACCTGCTCAGAAGGGCCCCACGG
TCACCACCAAGGTTCGGAGGACCATGAGCAGCCGTGTCCACGAGGCTGTGAAGGCCATTGCACTCTGCCA
CAACGTGACACCCGTGTACGAGTCCAATGGTGTGACGGACCAGGCTGAGGCTGAGAAGCAGTTTGAGGAC
TCCTGCCGAGTGTACCAGGCATCCAGCCCGGATGAGGTGGCTCTGGTCCAGTGGACAGAAAGTGTGGGAC
TGACGCTGGTGGGTCGAGACCAGTCCTCCATGCAGCTGAGGACCCCTGGTGACCAGGTCCTGAATCTCAC
TATCCTTCAGGTCTTCCCGTTCACCTATGAGAGCAAGCGGATGGGCATCATCGTGCGGGATGAGTCCACG
GGGGAAATCACGTTCTACATGAAGGGAGCAGACGTCGTCATGGCTGGCATTGTCCAGTACAACGACTGGC
TGGAGGAGGAGTGTGGCAACATGGCCCGGGAGGGACTACGTGTGCTGGTGGTAGCCAAGAAGTCCCTCAC
AGAGGAGCAGTACCAAGACTTTGAAGCCCGCTACGTCCAGGCTAAGCTGAGTGTGCATGACCGCTCGCTG
AAGGTGGCCACGGTGATCGAGAGCCTGGAGATGGAGATGGAGCTGCTGTGCCTGACTGGTGTGGAGGACC
AGCTGCAGGCAGATGTCAGGCCCACGCTGGAGACGCTGCGCAACGCTGGCATCAAGGTTTGGATGCTAAC
AGGGGACAAGCTGGAGACAGCCACGTGCACAGCCAAGAACGCACATCTGGTGACCAGAAACCAAGATATC
CATGTTTTCCGACTGGTGACCAACCGCGGGGAGGCCCACCTGGAGCTGAATGCCTTCCGTAGGAAGCATG
ACTGTGCCCTGGTCATCTCTGGAGACTCCCTGGAGGTTTGCCTCAAATACTATGAGTACGAGTTCATGGA
ACTGGCCTGCCAGTGCCCGGCTGTGGTGTGCTGCCGCTGTGCCCCAACCCAGAAGGCCCAGATTGTTCGG
CTGCTCCAGGAACGCACCGGGAAGCTCACCTGTGCAGTAGGGGACGGAGGCAATGACGTCAGCATGATCC
AGGAATCCGACTGCGGCGTGGGCGTGGAGGGCAAGGAAGGGAAGCAGGCCTCGCTGGCAGCGGACTTCTC
CATCACCCAGTTCAAGCATCTCGGCCGCTTGCTCATGGTGCACGGTCGGAACAGCTACAAGCGCTCGGCG
GCCCTCAGTCAGTTTGTGATCCACAGGAGCCTCTGCATCAGCACCATGCAGGCTGTCTTCTCGTCTGTGT
TCTACTTTGCATCCGTTCCTCTCTACCAAGGCTTCCTGATCATTGGGTATTCTACCATCTACACGATGTT
TCCCGTGTTCTCCCTGGTTTTGGACAAAGACGTGAAGTCGGAAGTCGCCATGTTGTATCCTGAGCTCTAC
AAGGACCTGCTTAAGGGGCGGCCACTGTCCTACAAGACGTTCTTAATTTGGGTGTTAATCAGCATCTATC
AAGGGAGCACCATCATGTACGGGGCGCTGCTGCTGTTCGAGTCGGAGTTTGTACACATCGTGGCAATCTC
CTTCACATCCCTCATCCTCACTGAGCTACTGATGGTGGCGCTCACCATCCAGACGTGGCACTGGCTCATG
ACAGTGGCCGAGCTACTCAGCCTGGCCTGCTACATTGCCTCCCTGGTGTTCCTCCATGAGTTCATCGATG
TCTACTTCATTGCCACCCTGTCATTCCTCTGGAAGGTGTCCGTCATCACCTTGGTCAGCTGTCTCCCCCT
CTATGTCCTCAAGTACCTGCGGAGACGGTTCTCCCCACCCAGCTACTCGAAGCTCACTTCCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_015731
Insert Size 3144 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015731.3, NP_056546.2
RefSeq Size 3529 bp
RefSeq ORF 3144 bp
Locus ID 11981
UniProt ID O70228
Cytogenetics 2 H3
Summary Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport. In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation. Appears to be implicated in intercellular communication by negatively regulating the release of exosomes. The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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"NM_015731" in other vectors (4)
SKU Description Size Price
MG211525 Atp9a (tGFP-tagged) - Mouse ATPase, class II, type 9A (Atp9a) 10 ug
$1,112.00
MR211525 Atp9a (Myc-DDK-tagged) - Mouse ATPase, class II, type 9A (Atp9a) 10 ug
$589.00 $912.00
MR211525L3 Lenti ORF clone of Atp9a (Myc-DDK-tagged) - Mouse ATPase, class II, type 9A (Atp9a) 10 ug
$1,212.00
MR211525L4 Lenti ORF clone of Atp9a (mGFP-tagged) - Mouse ATPase, class II, type 9A (Atp9a) 10 ug
$1,212.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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