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Micall2 (NM_174850) Mouse Untagged Clone

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SKU
MC223187
Micall2 (untagged) - Mouse MICAL-like 2 (Micall2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $967.00
8 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Micall2
Synonyms A930021H16Rik; JRAB; mFLJ00139; MICAL-L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223187 representing NM_174850
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCATAAAGGCCCTGCAAGAATGGTGCCGGCAGCAGTGCGAGGGCTACCGGGACGTGAGCATCA
CCAACATGACCACCTCGTTCCGCGATGGCCTGGCCTTCTGCGCTATCTTGCACCGACACCGGCCTGACCT
TATAAACTTCAGTGCCCTCCGGAAGGAAAACATTTACGAGAACAACAAGTTGGCCTTCCAAGTGGCTGAG
GAACAGCTGGGCATCCCAGCCCTGCTAGACGCTGAGGACATGGTGGCCTTGAAGGTTCCAGACCGGCTGA
GCATTCTCACCTATGTGTCACAATACTACAACTACTTCCATGGCCGGTCCCCAATTGGGGGCATGGCTGG
CATAAAGAGACCCTCATCCGATTCGACCGAAGAACTTTCTGGAAAGAAGGGCCTGTCCCAGCCTGCCAAG
TTGCCCTCGCCGGCTCAAACCCAGAGGTCACCACTGTCCCCAGCTCGGACAAACCCTGTGGTTCAGAGGA
ATGAAGGTGGCTCACAAAGACCATCCCCAAAGGCTGCTCCGGGGACCGCGGGAAGCTCTGTCAGCAGTAT
CTGTGGGGTCTGTGGCAAACATGTTCACCTTGTGCAGCGGCACCTGGCTGACGGGCGCCTCTACCACCGA
AGCTGCTTCAGGTGTAAACAGTGCTCCAGCACGCTGCACTCGGGGGCCTACCGTGCCACGGGAGAGCCCG
GAGTCTTCGTCTGTACCCACCACAGCTCAGAAGTCACCTCTGTGAGCCCCAAGTCATCAAACCTGGCCTC
CAGGAAACCAGGGGGTGTCACTGCAGACACCAGGCCATTCGGCGTCTCATGGACGGTTCAGGAGGCAAAT
GGAGAGGGGACACCACTGAGGGTCAGGACAGCAGCATGGGAACACGCTGGAGGAAACACAACCGCCAAAG
GTTTTGTCCAGACGGAACTTAAGCCACCATCTACCTCCCAAGTCCACGTGGGGAGCTCTGCGGGGCCCAA
GCTACCCACGATCACAGTGACTACTACTTCTGTGACTAGCAAAGCTCTCACCCATGTGACGAATAGCTCC
CCAATAGGATGGTCATCGCCTGCCCAGAGCAGCCCAGCGAACTTCAACTCCCGCCCAGTTGTATCCCCAA
GTGCTCGGAACACTCACCTACCTGGGTCCCAAGGGCAGACAGCCTCAAAAGGTGTTAAGACTCAGCTCAA
CTTGAACTCAGAGTCTTCAAACACAGCGGTCACCCCAGCCTGGACTTCCTCAGCCTCTAAGACCCAGCAG
GCCCGAGAGAAATTTTTCCAGACCCCTCCCTCGGCCCCAGCCCCAGCCTCAGCCCCAGCCCCAGCCCCCA
CCAGCAAGGTCCCCACTGTAGTGACTGTCCCCACCAGCAAGGTCCCCAATGTGGTGACTGCCCCCACCAG
CAAGGTCCCCACTGTAGTGACTGTCCCCACCAGCAAGGTCCCCACTGTGGTGTCTGCCCCCACCAGCAAG
GTCCCTACTGTGGTGTCTGCCCCCACCAGCAAGGTCCCCACTGTGGTGAATTCCACTAATAGCAGGGTCA
CCACGGTAGTAAATGCCCCCACCAGCAAGGTCCCCACTGTAGTGAGTGCCACAAATGGCAGGGTGCCCAC
TGTGGTGACTGCCCACACTGGTAGGGTCCCTGCTGTGATGAATACCTCTGCCAGCAAAGTCTCTCCTGTT
GTGGATGCCCCTGCCCAGGAGAGCAGCCGGGAGCAAGCATTAAGTGTCCTTAGGAAGGCCCTTCCGGCGC
TGACAGGATCTGGTACCCAGGCTCCAAACAGGTCCTTCCCAGCCACTTCCTCTGTCCTGGTAACCCTTCC
CAAGAATGAAGTGCCACAAAAAGTTCCCTCAGACAAGCTCTCAGCTTTAACCACCCAGACTCCTAATTTC
ACTATAAAGCTAGAGCCTTCAGCCCCAGTGAATGTGGGCAATACCGCTGTATTTCTCCAGGCTGGCAAAA
AGAGCCCGAGTATTTCTCCAAGGGTTGGTAAGACCAGCGTTGGCTCCAGGCCACAAGCAGAGGTGGCAGG
AGTAAAGGGTCCAGGTCCCATCTCTCAGGAAGGCCAAGAGGAGGGACCAGAAGGATGGAGGGCCCGGCTG
AAGCCTGTGGATAAGAAAACCCCTGCTGGGAGGTCTCTGGAGCAAAAAGAGCCAGTCCTGGCAGAGCCAA
GGATAGGTGACACTTCCAGGAAAGCCTCTAGTAGCTCTGACTCCAGCGTCCATATCACCTTGACTTCTAT
TCAGCACAAGAGGAAACCATGCCCTGCTGGTTCCGGGCCCAGTCCTGCAGCCCTCTCTCCTTCTCCATCA
CACCGCAAGAAACTAGCTGTCCCTCCCAGCCTTGATGTTTCAGCTGATTGGCTCCAGCCAGAGCCCAAGA
AGCAGGAAGATGGGACCAGGAGCTGTAAGGAGGAGAAGTCACCCACCAGGTGGTCAAGAGAGAGGTCTGC
AGTCCTGGACAGTGGCCTTGCTCCACCTGGTGAAGCTGTCACTTCCCCAGTCAGGCTCCACCCCGACTAT
ATCCCCCAGGAGGAGCTGCAGAGGCAGCTGCAGGACATTGAGAGCCAACTGGACGCCCTGGAGCTCAGGG
GTGTTGAGTTGGAGAAGCGGCTGCGGGCAGCTGAGGGAGATGCTTCTGAGGATAGCCTCATGGTGGACTG
GTTCCGGCTTATTCATGAGAAGCAGCTACTGTTGAGGCTGGAATCGGAACTCATGTACAAGTCCAAGGAC
CAGCGCCTGGAGGAGCAGCAGCTGGACCTCCAGGGTGAGCTGCGCAGGCTGATGGACAAGCCAGAGGGTC
TGAAGTCCCCCCAGGACCGCCAGCGGGAGCAGGAGCTGCTGAGCCAATACGTGAACACCGTGAATGACCG
AAGTGACATCGTTGACTTCCTGGATGAGGATCGGCTCCGGGAACAGGAAGAGGACCAAATGCTGGAGAAT
ATGATCCAGAACCTGGGCCTCCAGAGAAAGAAGTCCAAGTCCTTCTTGTCCAAGATCTGGTCCTCGAAGA
GCAAAAGCGGGCAGGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_174850
Insert Size 3030 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174850.3, NP_777275.2
RefSeq Size 3346 bp
RefSeq ORF 3030 bp
Locus ID 231830
UniProt ID Q3TN34
Cytogenetics 5 G2
Summary Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth.UniProtKB/Swiss-Prot Function
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"NM_174850" in other vectors (2)
SKU Description Size Price
MG215438 Micall2 (tGFP-tagged) - Mouse MICAL-like 2 (Micall2), (10ug). Note: ORF is codon optimized 10 ug
$1,166.00
MR215438 Micall2 (Myc-DDK-tagged) - Mouse MICAL-like 2 (Micall2). Note: ORF is codon optimized 10 ug
$966.00

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