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Heatr5b (BC086652) Mouse Untagged Clone

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SKU
MC223140
Heatr5b (untagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,029.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Heatr5b
Synonyms 2010013B10Rik; A230048G03Rik; AV071430; D330050P16Rik; mKIAA1414
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC086652
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTAGCTCACAGTTTATTGCTAAATGAGGAAGCTTTGGCTCAGATCACGGAGGCAAAGAGACCCG
TGTTCATCTTTGAATGGTTGAGATTTCTTGATAAAGTCCTAGTTGCTGCCAACAAGACAGATGTAAAAGA
AAAACAGAAAAAACTTGTTGAACAATTAACTGGATTAATAAGCAGCTCACCTGGACCACCTACTCGAAAA
TTGTTAGCTAAAAACCTTGCAGCCCTTTATAGCATTGGGGATACTTACACAGTTTTTCAAACACTAGATA
AATGTAATGACATTATCAGAAGTAAGGACGACACTGCAGCCTACTTACCAACAAAATTGGCAGCAGTGGC
TTGTGTTGGAGCCTTCTATGAGAAGATGGGAAGGATGTTGGGCAGTGCATTTCCAGAAACAGTGAACAAT
CTGTTGAAATCTCTGAAAAGTGCAGAGTCTCAAGGGCGAAGTGAGATCTTAATGAGTCTGCAGAAAGTGC
TGACTGGACTGGGCGGCGCAGCAGCTTCCTCTCACCGCGACATTTACAAGAATGCTCGATCCCTCCTGAC
CGACAGGTCGATGGCTGTCCGATGTGCAGTGGCCAAGTGCCTTCTGGAACTACAGAATGAAGCTGTGTTC
ATGTGGACTGCCGAACTAGAAAATGTAGCTACCCTCTGCTTTAAGGCTCTGGAAAACTCAAATTATGGGG
TACGAGTCGCAGTATCTAAGCTACTAGGAACAGTCATGGCTACAGCATTGATGCCGAAACAGGCAACAGT
CATGCGGCAAAATGTGAAGCGAGCAACCTTCGATGAAGTCTTAGAACTCATGGCCACGGGATTTCTGCGC
GGAGGGTCGGGTTTCCTAAAGAGTGGCGGAGAAATGTTGAAAGTTGGAGGGTCTGTTAATCGGGAAGTGA
GAGTTGGTGTGACACAGGCCTATGTTGTCTTTGTGACAACTCTGGGTGGCCAGTGGCTGGAGCGTAGCTT
TGCCACATTCTTGTCCCACGTACTTGATCTGGTTTCCCATCCTCGAGCAACACAAACACACGTGGACGCT
GTGTACTCCAGGCGATGTGTGTCCTTCATGCTGAGAGCTACCGTGGGCAGTCTGTTAGGTGAGAAAGCCC
AGATAGCAGCTGCCAAGGAGATCTGCCAGGCAATCGGAAAGCAGATGAAAGCTGTGGAAGCAGTAGTGAA
TGATACGAGCAGTGAAAACAAATCGGGGACAGCAGACATTGCAGCCAGCCAGCACGTCATGGTCTGTGCC
CTGCAGGAGCTTGGGAGCCTGGTGCAGAGCCTGAATGCCACCGCCTCTCCTCTTATTCAGGAAGCGTCCA
TAGGACTTTTGGAGATTGTGACGTCAGTGCTTCTTCACCCAAGCATGGCTGCTCGGCTTGCTGCTGCCTG
GTGTCTGCGTTGTGTAGCTGTGGCGCTGCCCTTCCAGTTGACACCATTTCTGGATAGGTGTGCAGAACGG
CTCAACAACCTCAAGACATCCCCAGAAGCTGTTAGTGGATACAGTTTTGCAATGGCCGCCTTGTTGGGTG
GAGTGCATCAGTGTCCTTTGGGCATTCCTCATGCCAAAGGAAAGATGGTGGTCAGCATTGCTGAAGATCT
GCTACGAACTGCTGCCCAAAACAGCAGGCTGTCCCTGCAGCGCACCCAGGCTGGCTGGCTTCTTCTGGGA
GCCCTCATGACTTTAGGACCGTCTGTTGTTCGTTACCATCTACCCAAGATGTTGCTGCTGTGGCGGAACG
TGTTCCCTCGCTCTTTAAAGGAACTGGAGGCTGAGAAGGCCCGGGGGGATTCATTCACCTGGCAGGTCAC
TCTGGAAGGTCGCGCTGGGGCTCTGTGTGCCATGAGGAGTTTTGTTGCACATTGTCCTGAGCTGCTAACA
GAGGATGCAATTAGGAAATTAATGACTCCTATCGAATGTGCCATGACCATGATGTCGCACATTCCATCCG
TCATCAAGGCCCATGGAGCCCATCTGAAAGCTAGTGCCGCAATGGTCCGCTTAAGACTTTATGATATCTT
GGCTTTATTACCTCCAAAAACTTATGAAGGGTCTTTCAATGCACTTCTTAGAGAGCTGGTGGCAGAGTTC
ACTCTGACTGACAACTCGGCCAACACAACTACCTCCCTCCTTCGCTCCCTCTGCCACTATGACGACAGCG
TTCTCCTGGGCTCCTGGCTTCAAGAGACTGATCATAAATCCATTGAAGACCAGCTCCAGCCAAACAGTGC
ATCTGGCAGTGGGGCTCTGGAACACGACCCTTCTTCCATTTACCTACGGATACCTGCAGGTGAAGCCGTG
CCGGGTCCGCTCCCTCTCGGAGTCTCAGTGATTGACGCATCTGTGGCTCTGTTCGGGGTTGTGTTTCCAC
ATGTTTCCTATAAACACAGGTTACAAATGCTGGATCACTTTGCTGAATGTGTTAAGCAAGCCAAAGGTGT
CCGCCAGCAGGCTGTGCAGCTTAATATATTTACTGCTGTTCTTAGTGCACTGAAGGGCTTAGCTGAAAAC
AAGAGTACTTTGGGACCTGAGGAAGTCCGCAAGTCTGCTCTGACACTGGTTATGGGAGCCCTCGACAACC
CAAACCCCATCTTACGGTGTGCGGCAGGGGAAGCCCTTGGAAGAATGGCTCAGGTGGTGGGAGAAGCCTC
TTTCATTGCCCGAATGGCCCAATACAGCTTTGACAAGTTGAAGTCAGCTCGAGATGTCGTGTCCAGGACT
GGTCACTCACTGGCCCTCGGTTGTTTACATCGTTATGTTGGTGGCATAGGATCAGGACAACACCTAAAGA
CCAGTGTCAGCATTTTACTGGCTCTAGCTCAAGATGGGACATCCCCTGAAGTTCAGACGTGGTCTCTTCA
TTCACTCGCCCTGATAGTGGATTCCAGCGGCCCAATGTACCGTGGGTACGTGGAGCCCACGTTGTCCCTG
GTACTCACCTTGCTGCTGACAGTTCCACCTTCACACACAGAAGTTCACCAGTGCCTGGGCAGATGCTTGG
GGGCGATCATAACTACTGTTGGACCAGAGCTGCAGGGAAATGCAGCGACCATATCCACCATCCGCTCCTC
CTGCTTGGTGGGCTGTGCCATTACCCAGGACCACTCTGACTCCCTTGTTCAGGCAGCTGCCATCTCTTGC
CTTCAGCAGCTTCACATGTTTGCCCCCACCACACATTCCACACCGACAATTAAGCTGAAAACAAGCTTTT
TTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC086652
Insert Size 3225 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC086652, AAH86652
RefSeq Size 3463 bp
RefSeq ORF 3224 bp
Locus ID 320473
Cytogenetics 17 E3
Summary Component of clathrin-coated vesicles (By similarity). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (By similarity).UniProtKB/Swiss-Prot Function
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"BC086652" in other vectors (4)
SKU Description Size Price
MG211590 Heatr5b (tGFP-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) 10 ug
$1,135.00
MR211590 Heatr5b (Myc-DDK-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) 10 ug
$589.00 $935.00
MR211590L3 Lenti ORF clone of Heatr5b (Myc-DDK-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) 10 ug
$1,235.00
MR211590L4 Lenti ORF clone of Heatr5b (mGFP-tagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641) 10 ug
$1,235.00

file-alt Citations

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