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Rnf40 (NM_172281) Mouse Untagged Clone

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SKU
MC223131
Rnf40 (untagged) - Mouse ring finger protein 40 (Rnf40), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $959.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Rnf40
Synonyms AI848422; BRE1-B; mKIAA0661
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223131 representing NM_172281
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGCCTCAGCAACAAGCGCGCCGCCGGCGACGGGGGCTCAGGACCCCCAGAGAAGAAGATGAACC
GCGAGGAGAAGACCACGACCACTCTAATCGAACCCATTCGTCTCGGCGGCATTTCTTCCACGGAGGAGAT
GGATTCCAAGGTACTGCAGTTCAAGAACAAGAAGCTGGCGGAGCGGTTGGAGCAGCGGCAAGCCTGCGAG
GATGAGCTCCGGGAGAGAATTGAGAAATTGGAGAAGAGGCAGGCCACTGATGATGCCACACTCCTCATCG
TCAACCGCTACTGGGCCCAGCTGGATGAAACTGTAGAAGCCCTCCTCCAGTGCTACGAGAACCAGAGGGA
ACTGTCTTCAGGGACAGAGGTGCCTGGGTGCCAAGAGGGCCTGACCCGTGATGTGATCCCTCGACCAGAC
CCAGGGACATCAGATCTGAGGGAACCGCTGCCAGTGCAGTTTCGAGCCCCTCTCAGTGAGCCGGCCTTGG
CTTTTGTGGTGGCACTGGGCGCCAGCAGCTGTGAGGAAGTGGAGCTGCAGCTGCAGGGTCGGATGGAATT
CTCCAAGGCTGCCGTGTCTCGGGTGGTCGAGGCCTCGGACCGCCTGCAACGGCAGGTGGAAGAACTCTGT
CAGCGAGTATACAGCCGGGGGGACAGTGAGGCCCCTGGGGAGGTGGCACGGGTGCGCACCCGGGAGCTAG
GCAGAGAGAACCGTAGGCTACAGGACTTGGCCACTCAGCTGCAAGAGAAACACCACCGCATCTCGTTGGA
GTACTCTGAGCTCCAAGATAAAGTGACATCGACAGAGACCAAGGTCCTAGAGATGGAGACCACAGTGGAG
GACCTGCAGTGGGACATTGAGAAGCTGCGCAAGCGAGAACAGAAGCTCAATAAGCACTTGGCAGAGGCCT
TGGAGCAGCTCAACTCCGGCTACTATGTGTCTGGGAGCTCCACAGGCTTTCAGGGAGGCCAGATTACACT
CAGCATGCAGAAGTTTGAGATGTTGAATGCAGAGCTGGAAGAAAATCAGGAATTGGCCAATAGCCGAATG
GCAGAGCTGGAGAAGCTGCAGGCGGAGCTTCAGGGAGCCGTTCGGACCAATGAGCGTCTCAAGGTGGCCC
TGCGTAGCCTTCCTGAGGAGGTCGTTCGTGAGACAGGGGAGTACCGCATGCTGCAGGCACAGTTCTCCCT
GCTCTACAATGAGTCTTTGCAAGTGAAGACCCAGCTCGATGAGGCCCGGGGGTTGCTGCTGGCCTCCAAG
AACTCCCACTTGAGACACATTGAACATATGGAGAGTGATGAATTGGGGCTGCAGAAGAAGCTGCGCACAG
AAGTTATCCAGCTGGAAGACACCCTGGCCCAGGTACGCAAGGAGTACGAGATGCTGCGCATCGAGTTTGA
ACAGAACCTTGCGGCCAACGAGCAGGCTGGGCCTATCAACCGGGAAATGCGCCACCTGATTAGCAGCCTC
CAGAACCACAATCACCAGCTAAAAGGAGATGCCCAGCGATACAAGCGGAAACTTCGAGAAGTACAGGCTG
AGATTGGTAAACTCCGGGCACAGGCCAGCGGCTCTTCCCACTGCATTCCCACCCTGAGCCACCCAGATGA
CCCTGGCCTCAATGCCCTTGCCCCAGGGAAGGAAGATAGTGGGCCAGGCCCTGGAGGTACCCCTGACTGC
AAGAAGGAGATGGCTTTATTGGCTGGAGCCACCTCTGCTACTTCTTCCATAAAGAAAGAGGAGCTGGTCT
CCTCTGAAGATGACGCCCAGGCCCTAACCCCTGTAACCCAGGGCTTGCCCTCCAGGGGTCGAGAACCTGA
GGCCAGACCCAAGCGAGAACTTCGGGAACGGGAAGGGCCTAGCCTAGGACCCCCACCTGCAGCCTCAACT
CTGTCAAGGGCTGATCGAGAAAAGGCCAAGGTGGAAGAAGCCAAAAGGAAGGAATCGGAACTGCTCAAAG
GTCTCCGAGCAGAGCTCAAGAAGGCTCAGGAAAGCCAGAAGGAGATGAAGCTGCTCCTGGACATGTACAA
GTCAGCACCCAAGGAGCAGCGGGACAAGGTGCAGCTCATGGCAGCTGAGCGCAAGGCCAAGGCTGAGGTG
GATGAGCTTCGGAGCCGCATCCGAGAGCTGGAAGAACGAGACCGAAGAGAAAGCAAGAAGATTGCGGATG
AAGATGCCCTGCGGCGGATCCGGCAGGCAGAAGAGCAGATTGAACACTTGCAACGCAAATTGGGTGCCAC
CAAGCAGGAGGAGGAGGCGTTGCTGTCGGAGATGGATGTAACAGGTCAGGCTTTTGAAGACATGCAGGAG
CAAAACGGGCGGCTACTTCAGCAGTTACGGGAAAAGGATGACGCCAACTTCAAGCTCATGTCGGAGCGGA
TCAAGGCCAACCAGATTCACAAGCTGCTCCGAGAGGAGAAGGATGAGTTGGGCGAGCAGGTTCTTGGCCT
TAAGTCCCAGGTGGATGCCCAGCTGCTGACCGTACAGAAGCTTGAGGAGAAGGAGCGGGCTCTGCAGGGC
AGCCTCGGGGGTGTGGAAAAGGAGTTGACTCTGCGCAGCCAGGCTCTGGAGCTTAATAAGAGAAAGGCTG
TAGAAGCAGCCCAGTTGGCTGAGGACCTGAAGGTGCAGTTGGAGCATGTGCAGACTCGACTTCGAGAGAT
CCAGCCCTGCCTGGCAGAGAGCCGGGCTGCCCGGGAGAAGGAGAGCTTCAACCTCAAGAGGGCTCAGGAG
GACATTTCCCGGCTTCGGCGAAAACTAGAGAAACAGCGGAAAGTGGAGGTTTATGCAGATGCTGACGAGA
TCCTGCAGGAGGAGATCAAGGAGTACAAGGCCCGGTTGACCTGCCCCTGCTGTAACACCCGCAAGAAGGA
TGCCGTGCTTACCAAGTGCTTCCACGTTTTCTGCTTTGAATGTGTGCGGGGCCGCTATGAGGCCCGCCAG
AGGAAATGTCCCAAGTGCAACGCAGCCTTTGGTGCCCACGACTTCCACCGTGTGTACATCAGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_172281
Insert Size 3006 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172281.2, NP_758485.2
RefSeq Size 4172 bp
RefSeq ORF 3006 bp
Locus ID 233900
UniProt ID Q3U319
Cytogenetics 7 F3
Summary Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.UniProtKB/Swiss-Prot Function
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"NM_172281" in other vectors (4)
SKU Description Size Price
MG211421 Rnf40 (tGFP-tagged) - Mouse ring finger protein 40 (Rnf40) 10 ug
$1,071.00
MR211421 Rnf40 (Myc-DDK-tagged) - Mouse ring finger protein 40 (Rnf40) 10 ug
$589.00 $871.00
MR211421L3 Lenti ORF clone of Rnf40 (Myc-DDK-tagged) - Mouse ring finger protein 40 (Rnf40) 10 ug
$1,171.00
MR211421L4 Lenti ORF clone of Rnf40 (mGFP-tagged) - Mouse ring finger protein 40 (Rnf40) 10 ug
$1,171.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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