Trp53bp1 (BC035206) Mouse Untagged Clone

SKU MC223081

Trp53bp1 (untagged) - Mouse transformation related protein 53 binding protein 1 (cDNA clone MGC:30704 IMAGE:3979746), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Trp53bp1
• Synonyms: m53BP1, 53BP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC035206; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGGAGATCAATGATCCTCTACTTGGGAATGAAAAAGGGGATTCTGAGTCTGCCCCAGAAATGGATG; GAAAACTGTCTCTGAAAATGAAACTGGTTAGTCCTGAGACAGAGGCCAGTGAAGAATCTTTGCAATTTAG; CCTGGAAAAGCCTACAACTGCTGAGAGAAAAAATGGATCTACTGCCATTGCAGAGCCTGTTGCCAGTCTC; CAGAAGCCGGTGCCTGTGTTTGGCTGCATCTATGAAGCCCAGCAAGAGAAGGAGGCTCAAAGTGAGGCTC; CTCCCTCTGCACCCGACAGGGCGAATTTGCTCCATTTTCCAAGTGCTCAAGAAGAAGACAAAGAAAGACC; AGATGTTACCCCAAACCTCAGGCAGAGTGAACAGCCTGTGAAGCCTGTTGGGCCTGTCATGGACGATGCT; GCTCCCGAGGACTCAGCTTCTCCTGTTTCGCAGCAGAGGGCCTCACAGGAGCCCTTCAGTCCTGCAGAGG; ACGTGATGGAAACAGACCTGCTGGAAGGACTGGCTGCTAACCAGGACAGACCTAGTAAGATGTTGATGGA; CAGGCCCACCCAGAGTAACATCGGGATCCAGACCGTGGACCATTCCCTGTGTGCCCCAGAAACTGTCTCG; GCAGCAACGCAGACTGTGAAGAGTGTGTGTGAGCAGGGGACCAGTACAGCGGAGCAGAACTCTGGGAAAC; AAGATGCCACTGTGCAGACTGAGAGGGGGAGTGGAGAGAAACCTGCCAGTGCTCCCGTGGACGACACAGA; GTCCCTCCACAGCCAGGGGGAAGAGGAATTTGAAATGCCCCAGCCTCCGCATGGCCATGTCTTGCATCGC; CACATGAGAACCATTCGAGAAGTCCGTACACTCGTCACCCGCGTCATCACAGATGTTTATTATGTGGATG; GGACAGAAGTGGAAAGGAAAGTAACGGAGGAGACTGAAGAACCAATCGTAGAGTGTCAGGAATGTGAAAC; AGAAGTTTCCCCTTCACAGACTGGAGGTTCCTCTGGGGACCTGGGGGACATCAGCTCCTTCTCCTCCAAA; GCTTCCAGCTCACACCACACATCCAGTGGAACAAGTCTCTCAGCCATACACAGCAGTGGCAGCTCAGGGC; GAGGAGCTGGGCCACTCAAAGGAAAAGCCAGTGGGACAGAAGCTGCAGATTTTGCCTTACCCAGTTCCCG; AGGAGGCCCAGGAAAACTGAGTCCTAGAAAAGGGATCAGTCAGACAGGGGCACCGGTGTGTGAGGAAGAT; GGTGATGCAGGCCTTGGCATCAGACAGGGAGGGAAGGCTCCAGTTACACCTCGTGGGCGTGGGCGAAGGG; GCCGCCCACCTTCTCGGACCACTGGAACCAGAGAAACAGTTGTCTCTGGTCCGTTGGGCGTAGAAGATAT; TTCACCTAGCATGTCACCAGATGACAAGTCCTTCACCCGCATTATGCCTCGTGTGCCAGATTCTACCAAA; CGGACGGATGCCAGTTCTAGTACTTTGCGGCGGAGTGATTCTCCAGAGATTCCTTTTCAGGCTGCTACTG; GTTCCTCTGATGGCTTGGATTCCTCATCTTCAGGAAACAGCTTTGTGGGTCTCCGTGTTGTAGCTAAGTG; GTCATCCAATGGCTACTTTTACTCTGGGAAGATCACCCGAGATGTGGGGGCTGGGAAGTACAAGCTGCTC; TTTGATGATGGGTACGAATGTGACGTGCTGGGCAAAGACATTCTCCTGTGTGACCCCATCCCCCTGGACA; CTGAAGTGACAGCCCTCTCAGAAGATGAATATTTCAGTGCAGGAGTGGTGAAAGGACATAGGAAGGAGTC; TGGGGAGCTGTATTACAGCATTGAAAAAGAAGGCCAAAGGAAGTGGTATAAACGAATGGCGGTCATTCTG; TCCTTGGAGCAAGGAAACAGACTGAGAGAGCAATATGGGCTTGGCCCATATGAAGCTGTCACACCGCTCA; CGAAGGCAGCAGATATCAGCTTAGACAATTTGGTGGAAGGAAAGCGGAAACGGCGCAGTAACATCAGCTC; CCCAGTCACCCCCACTGCCGCCAGTAGCAGCAGCACAACGCCCACACGTAAAGCCACAGAGAGTCCCCGT; GCTTCCACGGGAGTCCCGTCAGGCAAAAGGAAACTTCCGACTTCTGAAGAGGAACGCTCTCCTGCTAAGC; GAGGTCGAAAGTCTGCCACCGTGAAACCTGGAACAGTGGGGGCAGCAGAGTTTGTGAGTCCCTGTGAGAC; TGGAGACAACATAGGTGAGCCTTCTGTCCTAGAAGAGCCAAGAGGGCCTTTGCCCCTCAACAAGACCTTG; TTTCTGGGCTATGCCTTTCTCCTCACCATGGCTACAACTAGTGACAAGCTGGCCAGTCGCTCTAAGCTGC; TAGATGGTCCTACAGGAAGCAGTGAAGAAGAGGAGGAATTTTTAGAAATTCCTCCTTTCAACAAGCAGTA; TACAGAATGCCAGCTTCGAGCAGGAGCTGGGTATATCCTTGAAGACTTCAATGAAGCCCAGTGTAACACA; GCCTACCAGTGTCTCCTAATTGCGGACCAGCACTGTCGAACCCGGAAGTACTTCCTGTGCCTTGCCAGTG; GCATTCCTTGTGTGTCTCATGTCTGGGTCCATGACAGTTGCCATGCCAACCAACTTCAAAACTATCGTAA; TTATCTGCTGCCTGCTGGGTATAGCCTTGAAGAGCAACGAATTCTGGATTGGCAACCCCGTGAAAACCCT; TTCCAGAATCTGAAGGTCCTCTTGGTGTCAGATCAACAACAGAACTTCTTGGAGCTCTGGTCTGAGATCC; TCATGACTGGAGGGGCAGCCTCTGTGAAGCAGCACCATTCAAGTGCCCACAACAAAGACATTGCTTTAGG; GGTATTTGATGTGGTGGTGACAGACCCCTCATGCCCAGCCTCGGTGCTCAAGTGTGCTGAAGCCTTGCAA; CTGCCTGTGGTATCACAAGAATGGGTGATCCAGTGCCTCATTGTTGGGGAGAGAATTGGATTCAAGCAGC; ATCCAAAATATAAACATGATTATGTTTCTCACTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC035206
• Insert Size: 3045 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC035206, AAH35206
• RefSeq Size: 3441 bp
• RefSeq ORF: 3044 bp
• Locus ID: 27223
• Cytogenetics: 2 E5
• Summary: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:15159415, PubMed:15077110, PubMed:20453858, PubMed:23333305, PubMed:26308889, PubMed:20362325). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:23333305, PubMed:20362325). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:15159415, PubMed:15077110). Participates to the repair and the orientation of the broken DNA ends during CSR (PubMed:26308889). In contrast, it is not required for classic NHEJ and V(D)J recombination (PubMed:15159415). Promotes NHEJ of dysfunctional telomeres (By similarity).[UniProtKB/Swiss-Prot Function]