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Prpf6 (NM_133701) Mouse Untagged Clone

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SKU
MC222727
Prpf6 (untagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $949.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prpf6
Synonyms 1190003A07Rik; 2610031L17Rik; ANT-1; U5-102K
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222727 representing NM_133701
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAGAAGAAGAAGCCGTTCCTGGGCATGCCTGCGCCGCTGGGCTACGTACCCGGGCTGGGCAGGG
GTGCCACTGGCTTCACCACCCGATCAGACATCGGGCCTGCTCGGGATGCAAATGATCCTGTGGATGATCG
CCATGCCCCACCAGGGAAGAGAACTGTGGGGGACCAGATGAAGAAAAATCAGGCGGCTGATGATGATGAT
GAAGATCTGAATGATACCAACTACGATGAGTTTAATGGCTATGCTGGGAGCCTCTTCTCAAGTGGGCCCT
ATGAAAAAGATGATGAGGAAGCAGATGCTATTTATGCAGCTCTGGATAAAAGGATGGATGAGAGAAGAAA
AGAGAGAAGAGAGCAAAGGGAGAAAGAAGAGATTGAGAAATACCGAATGGAACGTCCCAAAATCCAGCAG
CAATTTTCAGACCTTAAGAGGAAGCTGGCAGAAGTCACAGAAGAAGAGTGGCTGAGCATCCCAGAAGTTG
GCGATGCCAGAAACAAACGTCAACGGAACCCACGTTATGAGAAGCTGACTCCTGTTCCTGATAGTTTCTT
TGCCAAACATTTACAGACTGGAGAGAACCATACTTCTGTGGATCCCCGACAGACTCAATTTGGAGGTCTG
AACACACCCTACCCGGGAGGCCTGAACACTCCATACCCAGGTGGAATGACACCTGGATTGATGACACCTG
GTACAGGGGAGCTGGACATGAGGAAGATTGGCCAGGCAAGGAATACCCTGATGGACATGAGGCTTAGCCA
GGTATCTGACTCAGTGAGTGGGCAGACTGTTGTGGACCCCAAAGGCTACCTGACAGATCTAAATTCCATG
ATCCCAACCCATGGCGGGGACATAAATGACATCAAGAAAGCACGACTACTTCTCAAATCTGTTCGAGAAA
CAAACCCTCATCACCCACCAGCTTGGATTGCATCAGCTCGCCTGGAAGAAGTCACTGGAAAGTTACAAGT
AGCTCGAAACCTTATTATGAAAGGGACAGAGATGTGCCCCAAGAGTGAAGATGTCTGGCTAGAAGCAGCT
AGGTTACAGCCTGGGGACACAGCCAAGGCTGTGGTGGCACAAGCTGTCCGTCATCTCCCGCAGTCTGTTA
GGATTTACATCAGAGCAGCAGAGCTAGAAACAGACATTCGAGCAAAGAAGCGTGTTCTTCGGAAAGCACT
TGAGCATGTTCCAAACTCTGTTCGATTGTGGAAAGCAGCTGTTGAGCTTGAAGAGCCTGAAGATGCTAGA
ATCATGCTGAGCCGAGCAGTAGAGTGCTGTCCCACCAGTGTAGAGCTATGGCTTGCTTTGGCAAGACTAG
AAACTTATGAAAATGCTCGCAAGGTCTTAAATAAAGCACGTGAGAACATTCCTACAGATCGGCACATCTG
GATCACAGCTGCCAAACTGGAGGAGGCCAATGGGAACACTCAGATGGTGGAAAAGATCATTGACCGAGCC
ATCACCTCCCTACGGGCCAATGGTGTAGAGATCAACCGCGAACAGTGGATCCAGGATGCTGAGGAGTGTG
ACAGGGCAGGGAGTGTGGCCACGTGCCAGGCTGTCATGCGTGCTGTAATCGGTATTGGGATTGAGGAGGA
AGATCGCAAACACACCTGGATGGAAGACGCTGACAGTTGTGTGGCCCACAATGCCCTGGAGTGTGCACGA
GCCATATATGCCTATGCCCTACAAGTCTTCCCCAGCAAGAAAAGTGTGTGGTTACGAGCTGCCTACTTTG
AGAAGAATCATGGTACCAGGGAGTCTCTGGAAGCACTCTTGCAGAGGGCTGTAGCCCACTGCCCCAAGGC
AGAGGTGCTATGGCTCATGGGTGCCAAGTCAAAGTGGCTGGCAGGGGATGTACCTGCAGCAAGGAGCATC
CTGGCCCTGGCCTTTCAGGCCAACCCCAACAGCGAAGAGATCTGGTTGGCGGCTGTGAAGCTGGAGTCAG
AGAACAATGAATACGAGCGAGCCCGGAGGCTACTGGCCAAGGCTCGGAGCAGCGCACCCACTGCCCGTGT
ATTCATGAAGTCTGTGAAGCTGGAGTGGGTTCTGGGGAACATCTCTGCGGCGCAGGAGCTGTGTGAGGAG
GCTCTGAGGCACTATGAAGATTTCCCCAAGCTGTGGATGATGAAGGGGCAGATTGAGGAGCAGGGAGAAC
TGATGGAGAAGGCCCGGGAGGCCTACAACCAGGGGCTGAAGAAATGTCCTCATTCCACACCGCTTTGGCT
CTTGCTTTCCCGACTAGAGGAAAAGATTGGGCAGCTGACACGTGCTCGGGCCATTTTAGAGAAATCTCGT
CTGAAAAACCCAAAGAACCCTGGGCTATGGTTGGAGTCAGTGCGGCTGGAGTACCGGGCAGGGCTGAAGA
ACATTGCCAATACCCTCATGGCCAAGGCGCTGCAGGAGTGCCCTAACTCTGGTATCCTGTGGTCTGAAGC
TGTCTTCCTTGAGGCAAGGCCCCAGAGAAAGACCAAGAGTGTGGATGCCCTGAAGAAGTGTGAACATGAC
CCCCATGTGCTTTTAGCTGTGGCTAAGTTGTTCTGGAGTGAAAGAAAGATCACCAAGGCTCGGGAGTGGT
TCCATCGCACTGTGAAGATTGACTCAGATCTGGGGGATGCCTGGGCCTTCTTCTACAAATTTGAACTGCA
GCATGGAACTGAGGAGCAACAGGAGGAGGTGAGGAAACGCTGTGAGAATGCAGAGCCCCGACATGGAGAG
CTGTGGTGTGCTGTGTCCAAGGATATCACCAACTGGCAGAGGAAGATTGGGGAAATCCTAGTCCTGGTGG
CCGCCCGCATCAAAAATACCTTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_133701
Insert Size 2826 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133701.2, NP_598462.1
RefSeq Size 3071 bp
RefSeq ORF 2826 bp
Locus ID 68879
UniProt ID Q91YR7
Cytogenetics 2 H4
Summary Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133701" in other vectors (4)
SKU Description Size Price
MG211260 Prpf6 (tGFP-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) 10 ug
$1,062.00
MR211260 Prpf6 (Myc-DDK-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) 10 ug
$589.00 $862.00
MR211260L3 Lenti ORF clone of Prpf6 (Myc-DDK-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) 10 ug
$1,162.00
MR211260L4 Lenti ORF clone of Prpf6 (mGFP-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) 10 ug
$1,162.00

file-alt Citations

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