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Msh2 (NM_008628) Mouse Untagged Clone

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SKU
MC222681
Msh2 (untagged) - Mouse mutS homolog 2 (E. coli) (Msh2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $943.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Msh2
Synonyms AI788990
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_008628
Insert Size 2808 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008628.2, NP_032654.1
RefSeq Size 3056 bp
RefSeq ORF 2808 bp
Locus ID 17685
UniProt ID P43247
Cytogenetics 17 57.87 cM
Summary Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.UniProtKB/Swiss-Prot Function
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"NM_008628" in other vectors (4)
SKU Description Size Price
MG211249 Msh2 (tGFP-tagged) - Mouse mutS homolog 2 (E. coli) (Msh2) 10 ug
$1,057.00
MR211249 Msh2 (Myc-DDK-tagged) - Mouse mutS homolog 2 (E. coli) (Msh2) 10 ug
$589.00 $857.00
MR211249L3 Lenti ORF clone of Msh2 (Myc-DDK-tagged) - Mouse mutS homolog 2 (E. coli) (Msh2) 10 ug
$1,157.00
MR211249L4 Lenti ORF clone of Msh2 (mGFP-tagged) - Mouse mutS homolog 2 (E. coli) (Msh2) 10 ug
$1,157.00

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