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Nrp1 (NM_008737) Mouse Untagged Clone

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SKU
MC222621
Nrp1 (untagged) - Mouse neuropilin 1 (Nrp1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,269.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nrp1
Synonyms C530029I03; NP-1; NPN-1; Npn1; Nrp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222621 representing NM_008737
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGGGGGCTGCCGTTGCTGTGCGCCACGCTCGCCCTTGCCCTCGCCCTGGCGGGCGCTTTCCGCA
GCGACAAATGTGGCGGGACCATAAAAATCGAAAACCCAGGGTACCTCACATCTCCCGGTTACCCTCATTC
TTACCATCCAAGTGAGAAGTGTGAATGGCTAATCCAAGCTCCGGAACCCTACCAGAGAATCATGATCAAC
TTCAACCCACATTTCGATTTGGAGGACAGAGACTGCAAGTATGACTACGTGGAAGTAATCGATGGGGAGA
ATGAAGGCGGCCGCCTGTGGGGGAAGTTCTGTGGGAAGATTGCACCTTCTCCTGTGGTGTCTTCAGGGCC
CTTTCTCTTCATCAAATTTGTCTCTGACTATGAGACACATGGGGCAGGGTTTTCCATCCGCTATGAAATC
TTCAAGAGAGGGCCCGAATGTTCTCAGAACTATACAGCACCTACTGGAGTGATAAAGTCCCCTGGGTTCC
CTGAAAAATACCCCAACAGCTTGGAGTGCACCTACATCATCTTTGCACCAAAGATGTCTGAGATAATCCT
GGAGTTTGAAAGTTTTGACCTGGAGCAAGACTCGAATCCTCCCGGAGGAATGTTCTGTCGCTATGACCGG
CTGGAGATCTGGGATGGATTCCCTGAAGTTGGCCCTCACATTGGGCGTTATTGTGGGCAGAAAACTCCTG
GCCGGATCCGCTCCTCTTCAGGCGTTCTATCCATGGTCTTTTACACTGACAGCGCAATAGCAAAAGAAGG
TTTCTCAGCCAACTACAGTGTGCTACAGAGCAGCATCTCTGAAGATTTTAAGTGTATGGAGGCTCTGGGC
ATGGAATCTGGAGAGATCCATTCTGATCAGATCACTGCATCTTCACAGTATGGTACCAACTGGTCTGTAG
AGCGCTCCCGCCTGAACTACCCTGAAAATGGGTGGACTCCAGGAGAAGACTCCTACAAGGAGTGGATCCA
GGTGGACTTGGGCCTCCTGCGATTCGTTACTGCTGTAGGGACACAGGGTGCCATTTCCAAGGAAACCAAG
AAGAAATATTATGTCAAGACTTACAGAGTAGACATCAGCTCCAACGGAGAGGACTGGATCTCCCTGAAAG
AGGGAAATAAAGCCATTATCTTTCAGGGAAACACCAACCCCACAGATGTTGTCTTAGGAGTTTTCTCCAA
ACCACTGATAACTCGATTTGTCCGAATCAAACCTGTATCCTGGGAAACTGGTATATCTATGAGATTTGAA
GTTTATGGCTGCAAGATAACAGATTATCCTTGCTCTGGAATGTTGGGCATGGTGTCTGGACTTATTTCAG
ACTCCCAGATTACAGCATCCAATCAAGCCGACAGGAATTGGATGCCAGAAAACATCCGTCTGGTGACCAG
TCGTACCGGCTGGGCACTGCCACCCTCACCCCACCCATACACCAATGAATGGCTCCAAGTGGACCTGGGA
GATGAGAAGATAGTAAGAGGTGTCATCATTCAGGGTGGGAAGCACCGAGAAAACAAGGTGTTCATGAGGA
AGTTCAAGATCGCCTATAGTAACAATGGCTCTGACTGGAAAACTATCATGGATGACAGCAAGCGCAAGGC
TAAGTCGTTCGAAGGCAACAACAACTATGACACACCTGAGCTTCGGACGTTTTCACCTCTCTCCACAAGG
TTCATCAGGATCTACCCTGAGAGAGCCACACACAGTGGGCTTGGGCTGAGGATGGAGCTACTGGGCTGTG
AAGTGGAAGCACCTACAGCTGGACCAACCACACCCAATGGGAACCCAGTGGATGAGTGTGACGACGACCA
GGCCAACTGCCACAGTGGCACAGGTGATGACTTCCAGCTCACAGGAGGCACCACTGTCCTGGCCACAGAG
AAGCCAACCATTATAGACAGCACCATCCAATCAGAGTTCCCGACATACGGTTTTAACTGCGAGTTTGGCT
GGGGCTCTCACAAGACATTCTGCCACTGGGAGCATGACAGCCATGCACAGCTCAGGTGGAGTGTGCTGAC
CAGCAAGACAGGGCCGATTCAGGACCATACAGGAGATGGCAACTTCATCTATTCCCAAGCTGATGAAAAT
CAGAAAGGCAAAGTAGCCCGCCTGGTGAGCCCTGTGGTCTATTCCCAGAGCTCTGCCCACTGTATGACCT
TCTGGTATCACATGTCCGGCTCTCATGTGGGTACACTGAGGGTCAAACTACGCTACCAGAAGCCAGAGGA
ATATGATCAACTGGTCTGGATGGTGGTTGGGCACCAAGGAGACCACTGGAAAGAAGGACGTGTCTTGCTG
CACAAATCTCTGAAACTATATCAGGTTATTTTTGAAGGTGAAATCGGAAAAGGAAACCTTGGTGGAATTG
CTGTGGATGATATCAGTATTAACAACCATATTTCTCAGGAAGACTGTGCAAAACCAACAGACCTAGATAA
AAAGAACACAGAAATTAAAATTGATGAAACAGGGAGCACTCCAGGATATGAAGGAGAAGGGGAAGGTGAC
AAGAACATCTCCAGGAAGCCAGGCAATGTGCTTAAGACCCTGGATCCCATCCTGATCACCATCATAGCCA
TGAGTGCCCTGGGAGTACTCCTGGGTGCAGTCTGTGGAGTTGTGCTGTACTGTGCCTGTTGGCACAATGG
GATGTCAGAAAGGAACCTATCTGCCCTGGAGAACTATAACTTTGAACTTGTGGATGGTGTAAAGTTGAAA
AAAGATAAACTGAACCCACAGAGTAATTACTCAGAGGCGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_008737
Insert Size 2772 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008737.2, NP_032763.2
RefSeq Size 5921 bp
RefSeq ORF 2772 bp
Locus ID 18186
UniProt ID P97333
Cytogenetics 8 75.78 cM
Summary Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis (By similarity). Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed:26503042).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008737" in other vectors (4)
SKU Description Size Price
MG211197 Nrp1 (tGFP-tagged) - Mouse neuropilin 1 (Nrp1) 10 ug
$1,467.00
MR211197 Nrp1 (Myc-DDK-tagged) - Mouse neuropilin 1 (Nrp1) 10 ug
$1,267.00
MR211197L3 Lenti ORF clone of Nrp1 (Myc-DDK-tagged) - Mouse neuropilin 1 (Nrp1) 10 ug
$1,567.00
MR211197L4 Lenti ORF clone of Nrp1 (mGFP-tagged) - Mouse neuropilin 1 (Nrp1) 10 ug
$1,567.00

file-alt Citations

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