Sun1 (NM_024451) Mouse Untagged Clone

SKU MC222597

Sun1 (untagged) - Mouse Sad1 and UNC84 domain containing 1 (Sun1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Sun1
• Synonyms: 4632417G13Rik; 5730434D03Rik; mKIAA0810; Unc84a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222597 representing NM_024451; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGACTTTTCTCGGCTGCACACGTACACCCCACCCCAGTGTGTGCCGGAGAACACTGGCTACACTTACG; CACTCAGTTCTAGTTACTCGTCGGATGCTCTGGATTTTGAAACTGAGCACAAGTTGGAACCTGTATTTGA; CTCTCCAAGGATGTCCCGCCGCAGCTTGCGTCTGGTCACAACAGCTTCGTACAGCAGTGGGGACAGCCAG; GCTATTGATTCGCACATTAGCACCAGCAGGGCCACCCCCGCCAAGGGGAGAGAAACCAGGACAGTCAAAC; AGAGAAGAAGTGCAAGCAAGCCAGCTTTTAGTATCAACCACCTGTCAGGGAAGGGCTTGTCCTCGAGCAC; AAGCCATGACAGCTCTTGCAGCCTGCGGAGTGCCACGGTGCTGCGGCACCCTGTGCTAGATGAGTCCCTG; ATTCGTGAGCAGACCAAAGTGGACCACTTCTGGGGTCTCGATGATGATGGTGACCTTAAAGGTGGAAATA; AAGCTGCCACTCAGGGAAATGGTGAACTGGCAGCAGAGGTGGCGAGCAGCAATGGATACACTTGCCGTGA; CTGCAGGATGCTCTCAGCGCGCACTGACGCACTCACAGCCCACTCTGCCATCCACGGGACCACCTCCAGG; GTGTACTCCAGAGACAGGACTCTCAAACCACGCGGAGTGTCCTTTTACCTGGATAGGACTCTGTGGCTGG; CCAAGTCCACCTCCTCATCCTTTGCATCATTTATAGTTCAACTTTTCCAAGTGGTTTTAATGAAGCTCAA; TTTTGAAACTTACAAATTGAAAGGCTATGAATCCAGAGCTTATGAATCACAGAGCTATGAGACAAAGAGC; CATGAGTCAGAAGCCCATCTCGGTCACTGTGGGAGGATGACTGCCGGAGAACTTTCCAGAGTGGACGGGG; AGTCCCTGTGCGATGACTGTAAGGGGAAGAAGCACCTTGAGATACACACAGCCACCCACTCGCAACTGCC; CCAGCCACACAGGGTGGCCGGGGCCATGGGGCGCCTCTGCATCTATACAGGTGACCTCTTGGTTCAAGCA; CTGCGAAGGACTAGAGCTGCCGGGTGGTCTGTGGCCGAGGCCGTGTGGTCGGTGCTCTGGCTGGCTGTCT; CTGCTCCAGGGAAGGCAGCCTCGGGAACCTTCTGGTGGCTAGGGAGCGGCTGGTACCAATTTGTTACTTT; GATTTCTTGGCTGAATGTCTTTCTTCTTACCAGGTGCCTTCGAAATATTTGCAAGGTTTTTGTCTTGCTC; CTCCCACTCCTACTTTTACTAGGTGCTGGTGTCTCCCTGTGGGGCCAGGGAAACTTCTTCTCACTCCTAC; CAGTGCTGAACTGGACGGCCATGCAGCCAACACAGAGGGTGGACGATTCCAAGGGCATGCATAGACCTGG; CCCTCTTCCCCCGAGCCCACCTCCAAAGGTTGATCACAAGGCTTCCCAGTGGCCTCAGGAGAGTGACATG; GGGCAGAAGGTAGCTTCTTTGAGTGCGCAGTGCCACAACCATGATGAGAGACTTGCAGAGCTGACAGTCC; TGCTTCAGAAACTACAGATACGGGTAGACCAAGTGGATGACGGCAGGGAAGGGCTGTCACTGTGGGTCAA; GAATGTGGTTGGACAGCACCTGCAGGAGATGGGCACCATAGAACCACCTGATGCTAAGACTGACTTCATG; ACTTTCCACCATGACCATGAAGTGCGTCTCTCCAACTTGGAAGATGTTCTTAGAAAACTGACAGAAAAAT; CTGAGGCTATCCAGAAGGAGCTGGAAGAAACCAAGCTGAAAGCAGGCAGCAGGGATGAAGAGCAGCCCCT; CCTTGACCGTGTGCAGCACCTAGAACTGGAACTGAACCTGTTGAAGTCACAGCTGTCAGACTGGCAGCAT; CTGAAGACCAGCTGTGAGCAGGCTGGGGCCCGCATCCAGGAGACTGTGCAGCTCATGTTCTCTGAGGATC; AGCAGGGCGGTTCCCTCGAGTGGCTATTAGAGAAGCTTTCTTCTCGGTTCGTGAGCAAGGATGAGCTGCA; GGTGCTCTTACATGACCTTGAGCTGAAACTGCTGCAGAATATCACACACCACATCACCGTGACAGGACAG; GCCCCGACATCCGAGGCTATTGTGTCTGCCGTGAATCAGGCAGGGATTTCAGGAATCACAGAAGCGCAAG; CACATATCATTGTGAACAATGCTCTGAAGCTGTACTCCCAAGACAAGACGGGGATGGTGGACTTTGCTCT; GGAGTCTGGAGGTGGCAGCATCCTAAGCACTCGGTGCTCTGAGACCTATGAGACCAAGACGGCACTGCTG; AGCCTGTTTGGGGTCCCACTGTGGTACTTCTCACAGTCACCTCGAGTGGTGATCCAGCCCGACATCTACC; CAGGGAATTGCTGGGCGTTCAAAGGTTCCCAGGGGTACCTGGTGGTGCGGTTGTCCATGAAGATCTACCC; AACCACATTCACCATGGAACACATTCCAAAGACACTATCACCCACTGGTAACATCTCCAGTGCCCCCAAA; GACTTTGCAGTCTATGGACTGGAAACGGAGTATCAAGAAGAGGGGCAGCCTCTGGGACGGTTCACCTATG; ACCAGGAAGGAGACTCACTCCAGATGTTCCACACACTGGAAAGACCTGACCAAGCCTTCCAGATAGTAGA; GCTCCGGGTCCTGTCCAACTGGGGCCACCCTGAGTACACTTGCCTCTACCGGTTCCGAGTCCACGGAGAG; CCCATCCAGTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024451
• Insert Size: 2742 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024451.2, NP_077771.1
• RefSeq Size: 4133 bp
• RefSeq ORF: 2742 bp
• Locus ID: 77053
• UniProt ID: Q9D666
• Cytogenetics: 5 G2
• Summary: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton (PubMed:20711465, PubMed:16380439, PubMed:24062341, PubMed:25892231, PubMed:26842404). The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (PubMed:19874786). Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration (PubMed:19874786). Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly (PubMed:17543860, PubMed:19211677, PubMed:19509342, PubMed:24062341, PubMed:25892231, PubMed:26842404). Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis (PubMed:17543860). Helps to define the distribution of nuclear pore complexes (NPCs) (PubMed:17724119). Required for efficient localization of SYNE4 in the nuclear envelope (PubMed:23348741). May be involved in nuclear remodeling during sperm head formation in spermatogenenis (PubMed:20711465). May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) is the longest transcript and encodes the longest isoform (1).