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Hace1 (NM_172473) Mouse Untagged Clone

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SKU
MC222549
Hace1 (untagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $916.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Hace1
Synonyms 1700042J16Rik; A730034A22Rik; BC025474
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222549 representing NM_172473
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAGCGATGGAGCAGCTCAACCGTCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGCTGC
CCGAGGATAATGAGACTGCTGTTTACACACTGATGCCAATGGTGATGGCCGACCAGCACAGGTCTGTTTC
TGAACTCCTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTGAAGAGAAGCTTGCTTCAC
ATTGCTGCAAACTGTGGATCTGTGGAATGCTTGGTTCTTCTGTTGAAGAAAGGCGCAAACCCTAACTATC
AAGATATTTCAGGCTGTACACCCCTTCATCTCGCTGCTAGAAATGGACAGAAGAAATGCATGAGTAAATT
ACTGGAGTACAGTGCGGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACACTGGCTGGCTGTG
AATGGGAGGACAGAGCTCCTCCATGACCTCGTGCAACACGTCACAGATGTGGATGTGGAGGATGCCATGG
GGCAGACAGCGCTACACGTAGCCTGCCAGAACGGGCACAAAACGACAGTGCAGTGCTTGCTAGACAGCGG
TGCTGATATTAACAGGCCGAATGTATCAGGAGCCACGCCACTGTACTTTGCTTGCAGTCATGGTCAGAGA
GATACTGCACAGATTCTTCTATTACGAGGAGCCAAATATTTACCAGATAAAAATGGAGTAACCCCTCTAG
ATCTATGTGTACAGGGTGGTTACGGACAGACTTGTGAAGTGTTAATTCAGTACCACCCGAGGCTTTTCCA
GACTATTGTTCAAATGACACAGAATGAAGACCTTCGGGAAAACATGTTACGGCAAGTTCTGCAGCATTTG
TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATCCTGACAGGCCTTGCTGAAGTTGCTACGACAAATGGCC
ATAAGCTGCTTAGTTTATCTAGCAATTATGATGCGCAGATGAAGAGCCTTTTGAGGATTGTGAGGATATT
TTGTCATGTCTTTCGAATTGGTCCCTCATCTCCCAGTAATGGAATCGATATGGGCTACAATGGAAATAAA
ACTCCAAGAAGCCAGGTGTTCAAGCCTTTGGAATTGCTTTGGCACTCATTGGATGAATGGTTAGTTTTAA
TAGCCACAGAATTAATGAAAAACAAAGAGGATTCAACAGATATTACTTCTATCTTGCTGAAACAAAAAGG
CCAGGATCAAGAGGCCCCTTCTCTTTCTGCATTTGAGCCACCAGGCCCTGGGAGCTATGAAAGCCTGCCC
CCTGGTCCAGGGGACTCCAAGCCAGAGGTGCTTGCAGGGGAGCAGGAAGCCAGTGCCGACTGTCAAGACG
TCATCTCTGTGACAGCCAACCGGCTGAGTGCTGTCATTCAAGCCTTCTACATGTGCTGTTCCTGTCAGAT
GCCTCCAGGAATGACTTCACCTCGTTTCATTGAGTTTGTCTGCAAGCATGATGAAGTATTGAAATGCTTT
GTGAATAGAAACCCCAAAATTATATTTGACCATTTTCACTTTCTTCTTGAATGTCCTGAATTGATGTCAA
GATTCATGCATATCATAAAAGCACAGCCATTTAAAGATCGATGTGAGTGGTTCTATGAACATCTGCACTC
AGGACAGCCGGACTCAGACATGGTCCACAGACCAGTGAGTGAGAACGATATCCTCCTGGTTCACAGAGAT
TCTATTTTTAGGAGTAGCTGTGAAATTGTATCAAAAGCAAACTGTGCAAAGCTAAAGCAGGGGATTGCTG
TACGATTTCATGGAGAAGAAGGCATGGGTCAAGGCGTTGTGCGTGAATGGTTTGATATTCTGTCTAATGA
GATCGTCAACCCTGACTATGCACTGTTCACCCAGTCAGCAGACGGAACAACTTTCCAGCCCAACAGCAAC
TCCTATGTCAATCCTGATCACTTGAACTATTTCCGGTTTGCTGGACAGATCTTGGGGTTAGCTTTGAACC
ACAGGCAGCTGGTCAATATTTACTTCACACGATCATTCTACAAGCACATTCTCGGTATTCCTGTAAATTA
CCAAGATGTAGCATCTATTGATCCTGAATATGCCAAAAATTTGCAATGGATTTTAGATAATGATATTAGT
GACTTGGGTCTAGAATTGACTTTTTCTGTTGAGACTGATGTATTTGGAGCTATGGAAGAGGTGCCTTTAA
AGCCTGGGGGTGGAAGTATTCTTGTGACACAAAATAACAAAGCGGAGTATGTCCAGCTCGTTACTGAACT
GCGTATGACAAGAGCCATTCAGCCGCAGATCAATGCGTTTTTACAGGGTTTTCATATGTTCATCCCGCCT
TCCCTCATACAACTGTTTGACGAGTATGAATTGGAGCTACTGCTTTCTGGCATGCCGGAAATTGATGTAA
ATGATTGGATAAAAAATACAGAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTG
GGAAGTTGTGGAAGACATTACTCAAGAAGAAAGAGTTCTTCTCTTGCAGTTCGTTACTGGCAGTTCCAGG
GTCCCGCATGGTGGCTTTGCCAATATTATGGGTGGAAGTGGTTTGCAAAACTTTACAATTGCTGCTGTGC
CATATACTCCAAATCTTTTACCAACTTCCAGCACATGCATCAACATGCTCAAGTTACCTGAATACCCAAG
TAAAGAAATACTCAAGGACAGACTCCTTGTAGCACTGCATTGTGGCAGCTATGGTTATACAATGGCATAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_172473
Insert Size 2730 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172473.3, NP_766061.2
RefSeq Size 3785 bp
RefSeq ORF 2730 bp
Locus ID 209462
UniProt ID Q3U0D9
Cytogenetics 10 B2
Summary E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity). May also act as a transcription regulator via its interaction with RARB.UniProtKB/Swiss-Prot Function
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Documents
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"NM_172473" in other vectors (4)
SKU Description Size Price
MG225877 Hace1 (tGFP-tagged) - Mouse HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (Hace1), (10ug) 10 ug
$1,033.00
MR225877 Hace1 (Myc-DDK-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1) 10 ug
$589.00 $833.00
MR225877L3 Lenti ORF clone of Hace1 (Myc-DDK-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1) 10 ug
$1,133.00
MR225877L4 Lenti ORF clone of Hace1 (mGFP-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1) 10 ug
$1,133.00

file-alt Citations

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