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Hace1 (NM_172473) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR225877L4
Lenti ORF clone of Hace1 (mGFP-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,133.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hace1
Synonyms 1700042J16Rik; A730034A22Rik; BC025474
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR225877).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172473
ORF Size 2727 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172473.3, NP_766061.2
RefSeq Size 3785 bp
RefSeq ORF 2730 bp
Locus ID 209462
UniProt ID Q3U0D9
Cytogenetics 10 B2
Summary E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity). May also act as a transcription regulator via its interaction with RARB.UniProtKB/Swiss-Prot Function
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"NM_172473" in other vectors (4)
SKU Description Size Price
MC222549 Hace1 (untagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), (10ug) 10 ug
$916.00
MG225877 Hace1 (tGFP-tagged) - Mouse HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (Hace1), (10ug) 10 ug
$1,033.00
MR225877 Hace1 (Myc-DDK-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1) 10 ug
$589.00 $833.00
MR225877L3 Lenti ORF clone of Hace1 (Myc-DDK-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1) 10 ug
$1,133.00

Plasmid Map for MR225877L4

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Cloning scheme for MR225877L4
Circular map for MR225877L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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