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Lgr5 (NM_010195) Mouse Untagged Clone

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SKU
MC222509
Lgr5 (untagged) - Mouse leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $831.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Lgr5
Synonyms FEX; Gpr; Gpr49
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222509 representing NM_010195
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACCTCCTGCGTCCACATGCTCCTGTCCTTGCTGGCGCTGCTGCAGTTGGTGGCCGCCGGCAGCT
CACCGGGACCAGATGCGATACCGCGGGGCTGCCCATCACACTGTCACTGTGAGCTGGATGGCAGGATGCT
GCTCAGGGTGGACTGCTCGGACCTGGGGCTCTCGGAGCTGCCCTCCAACCTCAGCGTCTTCACCTCCTAC
CTGGACCTCAGTATGAACAACATCAGTCAGCTACCCGCCAGTCTCCTACATCGCCTCTGCTTCCTAGAAG
AGTTACGTCTTGCTGGAAATGCTTTGACACACATTCCCAAGGGAGCGTTCACGGGCCTTCACAGCCTCAA
AGTGCTTATGCTGCAGAACAACCAGCTGAGACAGGTTCCGGAGGAAGCGCTACAGAATTTGAGAAGCCTT
CAATCCCTGCGCCTAGATGCCAACCACATCAGCTACGTGCCACCCAGCTGTTTCAGCGGCCTGCACTCCC
TGAGGCACCTGTGGCTAGATGACAATGCTCTCACAGACGTCCCTGTCCAGGCTTTCAGAAGTTTATCAGC
CCTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCACATAGCAGACTACGCCTTTGGAAACCTCTCC
AGCCTCGTGGTTCTGCATCTCCATAATAATAGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGACTCC
ACAGCCTGGAGACTTTAGATTTAAATTATAATAACCTTGATGAATTCCCCACTGCAATCAAGACACTCTC
CAACCTTAAGGAACTAGGATTCCACAGCAACAACATCAGGTCAATACCGGAGCGAGCGTTCGTAGGCAAC
CCTTCTCTTATCACAATACACTTCTATGACAACCCCATCCAATTTGTTGGAGTATCTGCTTTTCAGCATT
TGCCTGAACTAAGAACACTGACTTTGAATGGTGCCTCGCACATTACTGAATTTCCTCACTTGACAGGAAC
TGCCACCCTGGAGAGTCTGACTTTAACTGGAGCAAAGATCTCATCTCTTCCCCAGGCCGTCTGTGATCAG
TTACCTAATCTCCAAGTGCTAGATTTGTCTTACAACCTACTCGAAGACTTACCCAGTTTGTCAGGCTGCC
AAAAACTTCAGAAAATTGACCTGAGGCATAACGAGATCTATGAAATTAAGGGCAGCACTTTTCAGCAGTT
GTTTAACCTCCGATCTCTGAACTTAGCATGGAATAAAATTGCTATCATTCACCCCAATGCGTTTTCTACG
TTGCCGTCTCTAATAAAGTTGGACCTATCATCCAATCTCCTGTCGTCCTTCCCTGTGACTGGGTTACATG
GTTTAACTCACTTAAAATTAACAGGGAACCGAGCCTTACAGAGCCTGATACCATCTGCAAACTTCCCAGA
GCTCAAGATTATAGAAATGCCATCTGCTTACCAGTGTTGTGCATTTGGGGGGTGTGAGAATGTCTATAAA
ATTTCTAACCAATGGAATAAAGACGACGGCAACAGTGTGGACGACCTTCATAAGAAAGACGCTGGGTTAT
TTCAAGTTCAAGATGAGCGGGACCTTGAAGATTTCCTACTTGACTTTGAGGAAGACCTGAAGGCCCTTCA
CTCGGTGCAGTGCTCGCCTTCCCCAGGTCCCTTCAAGCCCTGTGAGCACCTATTTGGTAGCTGGCTGATC
CGAATCGGGGTGTGGACCACGGCAGTACTGGCGCTTTCCTGCAATGCCTTGGTGGCTTTGACCGTGTTCA
GAACTCCTCTGTACATCTCTTCCATAAAGCTGCTAATTGGGGTAATCGCGGTAGTGGACATTCTCATGGG
GGTCTCCAGTGCTGTGCTGGCTGCCGTGGATGCATTCACTTTTGGCCGTTTTGCTCAGCACGGTGCGTGG
TGGGAAGACGGAATCGGCTGCCAAATCGTTGGCTTCCTGTCCATTTTTGCTTCCGAATCGTCGATCTTCC
TGCTCACTCTGGCAGCGCTGGAACGAGGTTTTTCTGTCAAGTGCTCTTCGAAGTTTGAAGTGAAAGCTCC
CCTTTTTAGCCTGAGAGCGATCGTTTTGCTATGTGTCCTGTTGGCCCTGACCATTGCCACAATCCCCTTG
CTAGGAGGCAGTAAGTACAATGCCTCTCCCCTCTGCCTGCCCTTGCCCTTTGGGGAGCCCAGCACCACGG
GCTACATGGTGGCTCTCGTGTTGCTCAACTCTCTCTGTTTCCTCATAATGACCATTGCCTACACAAAGCT
CTACTGCAGTTTGGAGAAAGGAGAGCTGGAGAATCTTTGGGATTGTTCGATGGTGAAGCACATTGCTCTG
TTGCTCTTCGCCAACTGCATCCTTTACTGCCCCGTGGCTTTCTTATCCTTCTCCTCTTTGCTAAACCTCA
CCTTTATCAGTCCTGACGTCATTAAATTTATACTTCTCGTGATCGTCCCACTTCCTTCCTGTCTCAACCC
ACTTCTCTACATTGTCTTCAATCCCCATTTTAAGGAGGATATGGGCAGCCTGGGAAAGCATACCCGTTTC
TGGATGAGATCAAAACACGCGAGTCTGCTGTCCATTAACTCGGACGATGTTGAGAAACGGTCCTGTGAGT
CAACCCAAGCCTTAGTATCCTTTACCCACGCCAGCATAGCCTATGACTTGCCTTCCACTTCCGGGGCATC
ACCAGCTTACCCCATGACTGAAAGCTGTCATCTCTCTTCAGTTGCATTTGTCCCATGTCTCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010195
Insert Size 2724 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010195.2, NP_034325.2
RefSeq Size 4711 bp
RefSeq ORF 2724 bp
Locus ID 14160
UniProt ID Q9Z1P4
Cytogenetics 10 D2
Summary The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_010195" in other vectors (4)
SKU Description Size Price
MG219702 Lgr5 (tGFP-tagged) - Mouse leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10ug) 10 ug
$1,445.00
MR219702 Lgr5 (Myc-DDK-tagged) - Mouse leucine rich repeat containing G protein coupled receptor 5 (Lgr5) 10 ug
$1,245.00
MR219702L3 Lenti ORF clone of Lgr5 (Myc-DDK-tagged) - Mouse leucine rich repeat containing G protein coupled receptor 5 (Lgr5) 10 ug
$1,545.00
MR219702L4 Lenti ORF clone of Lgr5 (mGFP-tagged) - Mouse leucine rich repeat containing G protein coupled receptor 5 (Lgr5) 10 ug
$1,545.00

file-alt Citations

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