Enpp3 (NM_134005) Mouse Untagged Clone

SKU MC222344

Enpp3 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 3 (Enpp3), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Enpp3
• Synonyms: AI876438; CD203c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222344 representing NM_134005; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATTCCAGGCTAGCATTAGCCACAGAGGAGCCCATTAAGAAAGACAGTCTCAAGAAATACAAGATCC; TTTGTGTGGTTCTTCTTGCTTTGTTGGTGATTGTATCGCTTGGATTGGGGCTGGGACTGGGACTCAGGAA; ACCTGAAGAACAAGGCAGCTGCAGGAAAAAATGCTTTGACTCATCGCACAGAGGACTGGAGGGCTGCCGC; TGTGACTCGGGATGCACAGGACGAGGAGACTGCTGCTGGGACTTTGAAGATACCTGTGTGAAATCAACTC; AAATATGGACGTGCAATTTATTCCGTTGTGGGGAGAACAGACTGGAGACAGCCCTCTGTTCCTGTGCGGA; TGACTGTCTACAGAGGAAGGACTGCTGTGCTGACTACAAGACTGTGTGCCAAGGCGAAAGCCCATGGGTG; ACAGAAGCCTGTGCCTCATCCCAGGAGCCCCAGTGTCCACCAGGGTTTGACCTGCCACCAGTTATCTTGT; TCTCCATGGACGGTTTTAGAGCTGAGTATTTACAAACATGGAGTACCCTTTTGCCTAATATCAACAAACT; GAAAACATGTGGAATCCATTCTAAATATATGAGAGCTATGTATCCCACCAAAACCTTCCCAAATCATTAT; ACCATTGTCACGGGTTTGTATCCGGAGTCACACGGCATCATTGACAACAACATGTATGACGTGCACCTCA; ACAAGAATTTTTCCCTTTCTTCAGTGGAGAAAAGCAATCCCGCCTGGTGGAGCGGGCAGCCGATTTGGCT; AACGGCAATGTATCAAGGTTTAAAAGCTGCTTGCTACTACTGGCCGGGATCAGATGTGGCTGTCAATGGC; TCCTTCCCTACCATCTACAGGAATTACAGCAATTCTGTCCCATATGAAAGGAGGATTACAACACTGTTAC; AATGGCTGGACCTGCCCAAAGCTGACCGACCCAGTTTTTATACCATCTATGTGGAAGAGCCTGATTCTGC; AGGGCATTCGAGTGGACCAGTCAGTGCTGGAGTAATTAAAGCCTTGCAGTCAGTTGACAATGCCTTTGGA; ATGTTGATGGAGGGTCTGAAACAGAGGAATTTACACAACTGTGTCAATATAATCGTTCTGGCTGATCATG; GAATGGACCAGACCTCCTGTGATAGAGTGGAATACATGACAGATTATTTTCCTAAAATAAACTTCTATAT; GTATCAAGGACCTGCTCCTCGAATCAGGACTCGTAATATACCTCAGGACTTCTTTACATTTAATTCTGAA; GAGATTGTCAGGAACCTCAGTTGTCGAAAACCTGACCAACACTTTAAACCCTACTTGACCCCTGATTTGC; CGAAACGACTGCACTACGCCAAAAATGTCAGAATTGACAAAGCTCATCTCATGGTGGATCGGCAATGGCT; GGCTTTCAGGAGCAAAGGGAGTTCCAATTGTGGTGGAGGAACACATGGCTACAACAATGAGTTCAAGAGC; ATGGAGGCAATCTTTTTGGCACATGGACCCAGCTTTATAGAGAAGACTGTTATTGAACCATTTGAAAATA; TTGAAGTCTATAATCTACTGTGTGATCTTCTGCACATTGAACCAGCACCAAACAACGGTACTCATGGCAG; TCTGAATCATCTTCTGAAGACACCGTTTTACAAGCCATCACATGCAGGAGAGTTGTCAACGCCTGCTGAC; TGTGGTTTTACTACTCCCCTGCCTACAGACCCACTAGACTGCTCATGCCCTGCACTACAGAATACGCCTG; GTCTAGAAGAGCAAGCAAACCAAAGGCTAAATCTTAGCGAAGGCGAGGTTGCAGCAACGGTGAAAGCAAA; TTTGCCATTTGGGAGGCCCAGGGTGATGCAGAAGAACGGAGACCACTGTCTCCTTTACCACAGGGACTAC; ATCAGTGGGTACGGCAAAGCTATGAAGATGCCCATGTGGAGTTCATACACCGTTCTCAAGCCGGGAGACA; CATCATCTCTTCCTCCCACAGTTCCAGACTGTCTGCGGGCTGATGTCAGGGTTGCTCCTTCTGAGAGCCA; AAAATGTTCCTTCTATTTAGCAGACAAGAATATCACCCATGGCTTCCTCTATCCTGCAATCAAAGGAACT; AATGAGAGTCGATATGATGCCTTAATTACAAGTAATTTGGTCCCAATGTATAAAGAATTCAAAAAAATGT; GGGACTACTTCCATGAGGTTCTTCTTATAAAATATGCTATAGAAAGAAATGGACTGAATGTGGTTAGTGG; ACCCATCTTTGATTATAATTATGATGGCCATTTTGATGCTCCGGATGAAATCACACAATATGTAGCTGGC; ACTGATGTCCCTATCCCAACACACTACTTTGTGGTGCTGACAAGTTGTAAAGACCAAACCCACACACCCG; ACAGCTGCCCTGGGTGGCTGGATGTCCTGCCCTTCATCGTCCCTCACCGGCCCACCAACATAGAAAGCTG; TTCTGAAAACAAGACCGAGGACCTTTGGGTTGAAGAGAGGTTCCAAGCACACGCCGCCCGGGTCCGAGAT; GTGGAGCTCCTCACTGGCCTTGACTTTTATCAGGAGAAAGCACAGCCCGTCTCTCAGATTTTGCAACTAA; AGACGTATCTGCCCACGTTTGAAACCATTATTTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134005
• Insert Size: 2625 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_134005.2, NP_598766.2
• RefSeq Size: 2815 bp
• RefSeq ORF: 2625 bp
• Locus ID: 209558
• UniProt ID: Q6DYE8
• Cytogenetics: 10 A4
• Summary: Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (By similarity). Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines (PubMed:25692702). Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (PubMed:28225814). Has also alkaline phosphodiesterase activity (By similarity).[UniProtKB/Swiss-Prot Function]