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Ndst3 (NM_031186) Mouse Untagged Clone

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SKU
MC222336
Ndst3 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (Ndst3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $880.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ndst3
Synonyms 4921531K01Rik; 4930511P15Rik; N-HSST 3; NDST-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222336 representing NM_031186
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTTTATCATGAAACCTCACAGACACTTTCAGAGAACACTGATTCTGCTTGCCACCTTTTGTATGG
TAAGCATTATTATTTCTGCTTACTACCTGTACAGCGGCTACAAACAGGAAAGTGAAGTCTCCGGGCGGGC
TTCGGAAGTGGACTGTGGTGACCTCCAGCACATACCATCCAGGCTGATGGAAGTGAGGAGGACAATGATT
TCCGATGCTTCAAGGACAGACCCCACAGTCCTGGTGTTTGTGGAGAGCCAGTACTCATCCCTTGGTCAAG
ACATCATTATGATGCTAGAATCCATCCGGTTCCATTATCACACTGAAATCGCTCCTGGAAAAGGAGATCT
TCCGGCACTTACAGACAATGTGAAGGGCAAATATGTTCTCATTATATATGAGAATATTCTAAAGTATATA
AACATGGACTCTTGGAATAGAAGCCTTTTAGATAAATACTGTATAGAGTATGGTGTGGGTATCATTGGAT
TCCATAAAACCAGTGAGAAAAATCTACAGAGCTTTCAGTTCAGGGGCTTCCCCTTTTCCATAAGTGGAAA
CCTGGCAGTAAAAGATTGCTGTATTAATCCTCACTCCCCACTCCTTCGTGTGACCAAATCATCCAAGCTG
GACAGAGGTTCTCTACCTGGAACTGACTGGACAGTTTTTCAGATTAACCACTCCACCTACCAGCCAGTAA
TATTTGCCAAAGTTAAGACTCCAGAAAACCTTTCTCCTCCCATCTCTAAACATGCATTTTATGCCACTAT
CATACACGACCTGGGGCTTCATGACGGGATCCAGCGAGTTCTTTTTGGCAACAACTTGAACTTCTGGCTA
CACAAGCTCATCTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGCTGACACTGTCCTTGGACAGGT
ACATCCTTGTGGACATTGACGACATATTTGTAGGGAAGGAGGGCACAAGGATGAACACCAATGATGTGAA
GGCCCTGCTTGACACTCAGAATCTTTTGCGCACACAAATCACAAATTTTACATTCAACCTTGGATTTTCA
GGGAAATTTTATCACACAGGAACTGAAGAGGAAGATGAAGGGGATGATTGTCTGCTGGGTTCTGTGGACG
AGTTCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAGTCGTCTTTAAT
AGAGCAGATGATTCTCAACAAAAAGTTTGCCTTAGAGCACGGCATCCCTACCGATATGGGATATGCAGTG
TCCCCTCACCATTCAGGTGTCTACCCTGTCCATGTTCAGCTTTATGAGGCCTGGAAGAAGGTTTGGAATA
TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAACCAGCTAGATATCGGAGAGGCTTCATTCACAA
AAACATCATGGTTCTCCCAAGACAAACCTGTGGGCTCTTCACCCACACAATTTTCTACAAGGAGTATCCA
GGGGGTCCCAGGGAACTGGACAAGAGTATTCATGGAGGGGAGCTCTTCTTCACTGTGGTTCTCAACCCAA
TCAGTATTTTCATGACCCATTTGTCTAACTATGGAAACGACCGACTGGGATTATATACATTTGTGAATCT
AGCCAATTTTGTGCAGACCTGGACCAACCTGCGTCTTCAGACCTTGCCTCCAGCTCAGCTGGCTCACAAG
TATTTTGAGCTCTTTCCTGATCAGAAAGACCCTCTCTGGCAGAACCCCTGTGATGACAAACGCCACAGAG
ACATTTGGTCTAAAGAGAAAACCTGCGATCGTTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG
TACCACTGCCTTGTGTCTGTTCCTGATTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAATCC
TTTGAGGAGGTACAGTTCTTTAATAGAAATAACTACCACAGGGGGATTGATTGGTACATGGATTTCTTCC
CAGTCCCATCTAATGTCACCACTGACTTTCTGTTTGAGAAGAGTGCTAATTACTTCCATTCAGAGGACGC
TCCCAAGAGGGCTGCTTCTCTAGTCCCGAAAGCCAAAATCATCACCATACTCATTGACCCGTCAGACCGA
GCGTACTCCTGGTATCAGCATCAGCGATCCCATGAAGACCCTGCAGCTCTGAAATTTAGCTTCTATGAAG
TGATCTCTGCTGGGCCCAATGCACCCTGGGAACTCCGAACCCTGCAGAAGAGATGCCTGGTCCCTGGGTG
GTATGCCAACCACATCGAGAGATGGCTTGTTTATTTCCCTCCATTTCAGTTGCTAATCATTGATGGACAG
CAGCTAAGAACTACCCCTGCGACAGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTCTCACCTCATTATA
ATTACTCCGAAGCTTTAACGTTTGATTCTCATAAAGGCTTCTGGTGTCAGCTGCTGGAAGAAGGGAAAAC
AAAATGCCTTGGGAAGAGTAAAGGAAGAAAATACCCCCCGATGGACTCTGATAGCAGAGCGTTTCTGTCC
AGCTACTACCGCGACCACAACGTGGAGCTCTCGAAGCTGCTGCACAGGCTGGGGCAGCCTCTGCCCTCCT
GGCTGAGACAGGAGCTGCAGAAAGTGAGGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_031186
Insert Size 2622 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031186.3, NP_112463.2
RefSeq Size 5446 bp
RefSeq ORF 2622 bp
Locus ID 83398
UniProt ID Q9EQH7
Cytogenetics 3 G1
Summary Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_031186" in other vectors (4)
SKU Description Size Price
MG217087 Ndst3 (tGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (Ndst3), (10ug) 10 ug
$999.00
MR217087 Ndst3 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (Ndst3) 10 ug
$589.00 $799.00
MR217087L3 Lenti ORF clone of Ndst3 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (Ndst3) 10 ug
$1,099.00
MR217087L4 Lenti ORF clone of Ndst3 (mGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (Ndst3) 10 ug
$1,099.00

file-alt Citations

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