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Ago4 (NM_153177) Mouse Untagged Clone

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SKU
MC222171
Ago4 (untagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $868.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ago4
Synonyms 5730550L01Rik; AI481660; Eif2c4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222171 representing NM_153177
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCTGGGACCCGGACCTCCAGCCAGCCTCTTCCAGCCACCTCGGCGTCCTGGCCTTGGAACCG
TTGGGAAACCAATTCGACTGTTAGCCAATCATTTTCAGGTTCAGATCCCTAAAATAGATGTGTATCACTA
TGATGTGGATATTAAACCAGAAAAACGGCCTCGTAGAGTCAACAGGGAGGTGGTAGACACTATGGTGCGG
CACTTCAAGATGCAGATATTTGGAGATCGGCAGCCTGGCTACGATGGCAAAAGAAACATGTACACGGCAC
ACCCACTGCCAATCGGACGGGATAGGATTGATATGGAGGTGACCCTCCCAGGTGAGGGTAAAGACCAAAC
TTTCAAAGTGTCTGTGCAGTGGGTGTCAGTTGTGAGCCTTCAGCTGCTTTTAGAAGCTTTAGCAGGACAC
CTGAATGAAGTCCCAGATGACTCAGTACAAGCACTTGATGTGATTACAAGGCATCTCCCCTCTATGAGGT
ACACTCCAGTAGGCCGGTCTTTTTTCTCACCTCCTGAAGGTTATTACCACCCTCTGGGAGGGGGCAGAGA
GGTTTGGTTTGGCTTTCATCAGTCTGTGAGACCTGCCATGTGGAATATGATGCTCAATATTGATGTATCT
GCAACTGCTTTCTACCGGGCTCAACCTATCATTGAGTTCATGTGTGAGGTTTTAGACATTCAGAACATCA
ATGAGCAGACAAAACCTCTAACAGACTCCCAGCGTGTCAAGTTTACCAAAGAAATTAGGGGTCTTAAGGT
TGAGGTGACTCACTGTGGACAGATGAAACGAAAATATCGAGTTTGTAATGTGACAAGACGGCCAGCCAGT
CATCAAACCTTCCCTTTGCAGCTAGAGAATGGCCAAGCTATGGAATGTACAGTGGCTCAGTATTTTAAGC
AGAAGTATAGTCTGCAGCTGAAACACCCCCACCTTCCCTGCCTTCAAGTGGGGCAAGAGCAAAAGCATAC
CTACCTACCACTTGAGGTCTGTAATATAGTGGCAGGACAGAGATGTATAAAGAAGCTCACAGACAATCAG
ACATCCACCATGATCAAAGCCACGGCAAGATCTGCTCCTGACAGACAGGAGGAAATCAGTAGACTGGTGA
AGAGCAACAGCATGGTGGGTGGACCTGACCCATACCTGAAGGAATTTGGGATTGTTGTGCACAACGAGAT
GACAGAGCTCACAGGCAGGGTGCTTCCGGCTCCAATGCTGCAGTATGGAGGCCGGAATAAAACAGTAGCC
ACACCCAGCCAGGGTGTCTGGGACATGCGAGGAAAACAGTTTTATGCTGGCATTGAAATTAAAGTATGGG
CAGTGGCTTGTTTTGCGCCTCAGAAACAATGTAGGGAAGATTTGCTAAAGAGTTTCACTGACCAACTTCG
TAAAATCTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGTTTCTGCAAGTATGCTCAAGGTGCA
GACAGCGTGGAGCCCATGTTTAAACATCTGAAAATGACATATGTGGGCCTGCAGCTAATAGTGGTGATTT
TGCCTGGGAAGACACCAGTATATGCGGAGGTGAAGCGTGTTGGAGATACCCTTCTAGGTATGGCCACACA
GTGTGTCCAGGTCAAAAACGTTGTGAAGACCTCGCCCCAGACCCTTTCCAACCTTTGCCTGAAGATGAAC
GCAAAGCTGGGAGGGATTAACAATGTGCTTGTGCCTCACCAAAGGCCCTCAGTGTTCCAGCAGCCTGTCA
TCTTTCTGGGAGCAGATGTCACTCATCCGCCTGCTGGGGACGGGAAGAAGCCTTCTATTGCAGCCGTGGT
GGGCAGCATGGACGGCCATCCCAGCCGGTACTGTGCCACAGTCCGGGTACAGACATCTCGACAGGAGATC
ACCCAGGAGCTCCTCTACAGCCAGGAGGTCGTGCAGGACCTGACGAGCATGGCTCGGGAGCTGCTGATTC
AATTCTATAAGTCCACGCGCTTCAAGCCCACACGCATCATCTACTACCGCGGAGGGGTATCCGAGGGACA
GATGAAGCAGGTAGCTTGGCCGGAGCTAATAGCAATTCGAAAGGCATGTATAAGCTTGGAGGAAGACTAT
CGGCCGGGAATAACTTACATTGTGGTGCAAAAGAGGCACCACACACGACTCTTCTGTGCAGATAAAATGG
AAAGGGTGGGGAAAAGTGGCAATGTCCCAGCAGGCACCACAGTGGATAGCACCGTCACACACCCGTCTGA
GTTTGACTTTTACCTCTGTAGTCATGCAGGAATTCAGGGAACCAGCCGTCCTTCACATTACCAGGTCTTG
TGGGATGACAACTGCTTCACTGCAGATGAACTCCAGCTACTGACTTACCAGCTCTGTCACACCTACGTGA
GGTGCACACGCTCCGTCTCCATTCCTGCCCCTGCATATTATGCCCGGCTTGTGGCATTCCGGGCAAGGTA
TCATTTGGTGGATAAAGATCATGACAGTGCGGAAGGCAGTCACGTGTCAGGACAGAGCAACGGCCGGGAT
CCTCAGGCCTTGGCTAAGGCTGTGCAGATCCACCATGATACCCAGCACACTATGTATTTTGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_153177
Insert Size 2586 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153177.3, NP_694817.2
RefSeq Size 6527 bp
RefSeq ORF 2586 bp
Locus ID 76850
UniProt ID Q8CJF8
Cytogenetics 4 D2.2
Summary Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.UniProtKB/Swiss-Prot Function
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"NM_153177" in other vectors (4)
SKU Description Size Price
MG210988 Ago4 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) 10 ug
$989.00
MR210988 Ago4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) 10 ug
$589.00 $789.00
MR210988L3 Lenti ORF clone of Ago4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) 10 ug
$1,089.00
MR210988L4 Lenti ORF clone of Ago4 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) 10 ug
$1,089.00

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