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Usp5 (NM_013700) Mouse Untagged Clone

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SKU
MC222154
Usp5 (untagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $865.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Usp5
Synonyms AA407472; ISOT; ISOT-1; Ucht
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222154 representing NM_013700
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCTGAGTGAAGAGGCGCTGCTGTCAGTGTTACCGACGATCCGTGTCCCCAAGGCGGGAGACC
GGGTCCATAAAGACGAGTGCGCTTTCTCTTTCGACACGCCGGAGTCTGAGGGTGGCCTCTATATCTGCAT
GAACACATTCCTGGGATTCGGGAAGCAGTATGTGGAGAGACACTTCAACAAGACAGGCCAGCGTGTCTAC
CTGCACCTCCGGAGGACCCGGCGACCGAAAGAAGAGGACACCAGTGCAGGCACTGGAGACCCACCTCGGA
AGAAGCCCACCCGGCTGGCCATTGGTGTTGAAGGAGGGTTTGACCTCACCGAGGACAAGTTTGAATTTGA
CGAGGATGTGAAGATTGTCATTTTGCCCGATTACCTGGAGATCGCTCGGGATGGGTTGGGGGGGCTTCCT
GACATTGTCAGAGATCGGGTGACCAGTGCTGTGGAAGCCCTACTTTCTGCTGACTCAGCCTCTCGAAAGC
AGGAGGTCCAGGCTTGGGATGGGGAAGTCCGTCAGGTGTCCAAGCATGCCTTCAACCTCAAGCAGTTGGA
CAACCCTGCTCGGATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGCGACATGCGCGAGAACCTGTGGCTC
AACCTGACAGACGGCTCCATCCTCTGTGGACGGCGCTACTTCGATGGCAGTGGGGGCAACAACCATGCTG
TGGAGCACTACAGGGAGACTGGCTACCCCTTAGCTGTTAAGCTGGGCACCATCACCCCGGATGGAGCTGA
CGTGTATTCTTACGACGAGGATGACATGGTTCTGGACCCCAGCCTGGCAGAGCACTTGTCTCACTTTGGC
ATCGACATGCTGAAGATGCAGAAGACGGACAAGACGATGACTGAGCTGGAGATAGACATGAACCAGCGGA
TTGGTGAATGGGAGTTGATCCAGGAGTCAGGCGTGCCACTCAAGCCTCTGTTTGGGCCTGGTTACACGGG
CATCCGGAACCTGGGTAACAGCTGCTATCTCAACTCCGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTT
CAGAGGAAGTATGTGGATAAATTGGAGAAGATCTTCCAGAATGCCCCAACGGACCCCACCCAAGACTTCA
GCACCCAGGTGGCCAAGCTGGGGCATGGCCTTCTCTCTGGAGAATACTCTAAGCCAGCACTGGAGTCAGG
TGATGGGGAGCAGGTACCAGAACAAAAGGAAGTTCAAGATGGCATTGCCCCTCGAATGTTCAAGGCCCTC
ATTGGCAAGGGCCACCCTGAGTTCTCCACTAATCGGCAGCAGGATGCCCAGGAATTCTTCCTACATCTTA
TCAACATGGTGGAGAGGAATTGCCGGAGTTCTGAAAATCCAAATGAAGTGTTCCGCTTTTTGGTGGAAGA
AAAGATCAAATGCCTGGCCACAGAGAAGGTTAAGTACACTCAGCGAGTTGACTACATCATGCAGTTGCCT
GTACCCATGGATGCAGCCCTTAACAAAGAGGAGCTTTTAGAGTATGAAGAGAAGAAGCGGCAAGCTGAAG
AGGAGAAAGTGCCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCCTGCCTGGAAGCCTACGG
GGCCCCTGAGCAGGTGGACGACTTCTGGAGTACAGCTCTACAGGCGAAGTCGGTCGCTGTCAAGACCACG
CGCTTTGCCTCCTTCCCCGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTTGGCTTAGACTGGGTGC
CCAAGAAGCTGGATGTGTCCATTGAGATGCCAGAGGAGCTCGATATCTCCCAGCTGAGGGGCACAGGGCT
ACAGCCGGGAGAGGAGGAGCTGCCTGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC
CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCGACATCACCCA
TGCTGGACGAATCCGTCATCATACAGTTGGTGGAAATGGGCTTCCCTATGGATGCCTGCCGGAAAGCTGT
GTACTATACGGGAAACAGCGGGGCCGAAGCAGCCATGAACTGGGTCATGTCACACATGGATGATCCAGAT
TTTGCAAACCCCCTCATCCTGCCTGGCTCCAGTGGGCCTGGCTCCACAAGTGCAGCAGCTGACCCCCCGC
CGGAGGACTGTGTGACCACCATAGTCTCCATGGGCTTCTCCAGGGACCAGGCCCTGAAAGCTCTGCGGGC
CACGAACAATAGCTTAGAGCGGGCTGTAGACTGGATCTTCAGCCACATTGATGACCTGGACGCAGAAGCT
GCTATGGACATCTCAGAGGGACGCTCAGCGGCTGAGTCCATCTCCGAGTCTGTGCCAGTGGGACCTAAAG
TCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACATCTACTATGTGTGG
TCACTATGTCTGCCATATCAAGAAGGAAGGCAGGTGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCT
GAAAAGCCACCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGTCAGCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_013700
Insert Size 2577 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013700.2, NP_038728.1
RefSeq Size 3233 bp
RefSeq ORF 2577 bp
Locus ID 22225
UniProt ID P56399
Cytogenetics 6 59.17 cM
Summary Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_013700" in other vectors (4)
SKU Description Size Price
MG210971 Usp5 (tGFP-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) 10 ug
$986.00
MR210971 Usp5 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) 10 ug
$589.00 $786.00
MR210971L3 Lenti ORF clone of Usp5 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) 10 ug
$1,086.00
MR210971L4 Lenti ORF clone of Usp5 (mGFP-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) 10 ug
$1,086.00

file-alt Citations

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