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Prickle1 (NM_001033217) Mouse Untagged Clone

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SKU
MC221957
Prickle1 (untagged) - Mouse prickle homolog 1 (Drosophila) (Prickle1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $839.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prickle1
Synonyms 1110058P22Rik; AW215793; b2b019Clo; mpk1; Prickle
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221957 representing NM_001033217
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTTTGGAGATGGAACCGAAAATGAGCAAACTGGTCTTCGGGTGCCAGAGAAGCTCCACATCAGATG
ATGATTCCGGCTGTGCACTTGAAGAGTATGCATGGGTCCCACCGGGCCTCAGGCCCGAGCAGATCCAGCT
CTATTTCGCATGCTTGCCAGAGGAAAAGGTTCCTTATGTTAACAGCCCTGGGGAGAAACACAGGATTAAA
CAGCTCCTGTACCAGTTGCCTCCACACGATAACGAGGTGCGGTACTGCCAGTCTTTGAGCGAAGAAGAGA
AGAAGGAGCTGCAGGTGTTCAGTGCTCAGAGGAAGAAAGAAGCTCTGGGGAGAGGAACCATCAAACTCTT
GTCCAGAGCTGTGATGCACGCCGTGTGTGAGCAGTGTGGGCTGCAGATGAATGGAGGGGAGGTGGCCGTG
TTCGCCTCCCGTGCAGGACCTGGAGTATGCTGGCACCCGTCCTGCTTTGTCTGCTTCACGTGTAATGAAC
TACTGGTCGACCTCATCTATTTCTATCAGGATGGAAAAATCCACTGTGGCAGGCACCACGCTGAACTGCT
CAAGCCTCGGTGCTCAGCCTGTGATGAGATCATTTTTGCTGACGAGTGCACGGAAGCCGAGGGTCGCCAC
TGGCACATGAAGCATTTCTGCTGTCTGGAATGTGAGACAGTCCTGGGAGGGCAGAGATATATCATGAAGG
ACGGCCGCCCCTTCTGCTGTGGCTGCTTCGAGTCTCTCTACGCTGAGTACTGCGAAACCTGTGGGGAGCA
TATTGGTGTGGACCACGCACAGATGACCTACGACGGGCAGCACTGGCACGCCACAGAGGCCTGCTTTTCC
TGTGCGCAGTGTAAGGCCTCGTTGCTGGGATGCCCCTTCCTCCCGAAACAAGGTCAGATTTACTGCTCCA
AGACCTGCAGCCTTGGGGAAGACATCCACGCCTCTGACTCCTCGGACTCCGCCTTCCAGTCAGCCAGATC
CAGAGACTCTCGCAGGAGCGTGAGGATGGGCAGAAGCAGCCGCTCCGCCGACCAGTGCCGACAGTCTCTT
CTCTTGTCTCCCGCCCTGAACTACAAGTTCCCGGGTCTCTCCGGCAACGCTGACGACACCCTTTCCCGGA
AGCTGGATGACGTGAGCCTCGCCAGCAGGCAGGGAGCAGGTTTCGCTAACGAGGAATTCTGGAAAGCCAG
AGTGGAGCAGGAAGCCTCCGAAGACCCCGAAGAATGGGCTGAGCATGAAGATTATATGACGCAGCTCCTC
CTCAAGTTCGGCGACAAAAACCTCTTCCAGCAGCAGAGCAGCGAGGTGGATCCGAGAGCCAGCGAGCACT
GGATACCCGACAACATGGTTACGAATAAGCCCGAGGTAAAGCCGAATCACCAGGGCCTCGCGAGTAAAAA
GTATCAGTCTGATATGTACTGGGCCCAGTCCCAAGACGGGCTGGGTGACTCTGCCTACGGCAGCCATCCA
GGCCCCGCCAGCAGCCGCAGGCTGCAAGAGCTAGATCTGGACCACGGTGCTGCGGGATATACTCATGACC
AAAGCCAGTGGTATGAAGACTCCCTGGAGTGTCTATCTGACTTGAAACCAGAACAGAGTATCCGGGATTC
TATGGATTCTTTGGCGTTGTCCAACATCACAGGGGCATCAGTGGATGGAGAAAGCAAGCCAAGACCGTCA
TTATATTCTCTCCAAAACTTTGAGGAGATAGAGGCAGAAGATTGTGAGAAAATGAGCAATATGGGAACTC
TGAACTCTTCCATGCTGCACAGGAGTGCAGAATCCTTACAAAGTCTGAACTCAGGGCTGTGCCCAGAAAA
AATTCTACCTGAGGAAAAACCAGCACACCTGCCGGTGCTCAGGAGATCCAAGTCCCAGTCCCGACCACAG
CAGGTCAAATTTTCAGATGACGTCATTGACAACGGAAGCTACGACATCGAAATCCGGCAGCCTCCCATGA
GCGAACGGACTCGGAGACGGGCGTATCACTTTGAAGAGAGGGGATCCAGGCCTCACCACCATCGCCACCG
GAGAAGCAGAAAGTCTCGGTCCGACAACGCCCTGAACCTGGTCACGGAAAGGAAGTACTCCGCCAAGGAC
AGACTTCGGCTGTACACCCCCGATAACTATGAGAAATTCATACAGAATAAAAGCGCCCGGGAGCTCCAAG
CCTACATGCAGAACGCCAACCTCTATAGCCAGTATGCCCATGCCACGTCTGATTACGCCCTGCAGAACCC
AGGGATGAACCGGTTTCTGGGGCTCTGCGGCGAGGACGACGACTCGTGGTGTTCGTCCTCCACGTCCTCC
TCCGACTCAGAAGAAGAAGGCTATTTCCTTGGCCAGCCGATCCCTCAGCCACGGCCACAGAGATTCACCT
ACTATACAGATGACCTTTCTAGCCCGGCTTCCGCACTGCCCACCCCACAGTTTACTCAGAGGACAACTAA
ATCCAAGAAGAAAAAAGGACACAAGGGCAAAAACTGTATCATTTCTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001033217
Insert Size 2499 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033217.4, NP_001028389.1
RefSeq Size 4085 bp
RefSeq ORF 2499 bp
Locus ID 106042
UniProt ID Q3U5C7
Cytogenetics 15 E3
Summary Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure (By similarity). Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033217" in other vectors (4)
SKU Description Size Price
MG217339 Prickle1 (tGFP-tagged) - Mouse prickle homolog 1 (Drosophila) (Prickle1), (10ug) 10 ug
$962.00
MR217339 Prickle1 (Myc-DDK-tagged) - Mouse prickle homolog 1 (Drosophila) (Prickle1) 10 ug
$589.00 $762.00
MR217339L3 Lenti ORF clone of Prickle1 (Myc-DDK-tagged) - Mouse prickle homolog 1 (Drosophila) (Prickle1) 10 ug
$1,062.00
MR217339L4 Lenti ORF clone of Prickle1 (mGFP-tagged) - Mouse prickle homolog 1 (Drosophila) (Prickle1) 10 ug
$1,062.00

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