Dsc1 (NM_013504) Mouse Untagged Clone

SKU MC221930

Dsc1 (untagged) - Mouse desmocollin 1 (Dsc1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Dsc1
• Synonyms: 1110020A10Rik; AI507491; Dsc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221930 representing NM_013504; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGTGGCCTGTGCTGCCCCAGGGAGCACCTTCTCTAAGCAGCTCCTTTTCTTTCTCCTGGTTCTGG; TATTATTTTGTGATGCCTGTCAAAAAGTTTCCCTTCACGTTCCCTCTCATCTTAAGGCTGAAACACCTGT; AGGCAAAGTGAATCTGGAAGAGTGTCTCAAATCACCCAGCCTGATCCTGTCCAGTGACCCAGCCTTCAGA; ATTCTAGAAGACGGCACAATTTACACAACACATGACCTGCTTTTGTCTTCTGAAAAGAGGGGGTTCTCCA; TCTTGCTCTCAGATGGTCAAGGGCAGGAACAAAAGAAGCTAGAAGTTGTACTGTCAGCAAGAGAAAAAAA; GGTTTTTAGGAAAAGACATACCAAAGAGCCAGTACACAACCGCAGTAAGAGAAGATGGGCTCCTATTCCA; TGTTCACTGATGGAGAACTCTTTGGGTCCATTTCCACAACACATTCAGCAGATCCAATCTGATGCTGCCC; AGAATTACACCATCTTTTACTCTATCAGTGGACCAGGAGTAGACAAAGAGCCTTACAATTTGTTCTACAT; AGAGAAAGACACCGGGGACATCTATTGTACCCGAAGCATTGACCGTGAACAGTATGACCAATTTTTGGTA; TATGGATATGCAACAACTGCTGATGGCTATGCCCCAGACTACCCTCTCCCCCTGCTGTTTAAGGTTGAAG; ATGACAATGACAATGCCCCATATTTTGAAACCAAATTAACAGTTTTTAGTGTGCCTGAAAATTGCCGATC; TGGAACTTCAGTGGGACAAGTGACTGCCATAGACAAGGATGAGCCAGGAACTCTACACACTCGTCTGAAG; TACAAAATCTTACAACAAATCCCAGACCAGCCAAAGCACTTCTCCATCCACCCAGACACAGGTGTGATCA; CTACCACCACACCTTTACTGGACAGAGAGAAATGTGACACTTACAAGCTAGTGATGGAGGTACGAGACAT; GGGGGGCCAGCCCTTTGGTCTGTTCACCACAGGAACAATCACCATCTCACTGGAGGATGAGAATGACAAC; TCGCCATACTTCACCCAAACTTCTTATACTACAGAAGTAGAAGAAAACAGAATTGATGTTGAGATTCTGA; GAATGGTGGTGCACGACCAAGATTTGCCCAACACCCCCCACTCGAAGGCGGTATACACAATCCTAAAGGG; GAACGAAAACGGGAACTTCAAAATCACAACGGATCCAAATACAAATGAAGGGGTCTTGTGTGTTGTCAAG; CCACTGAACTATGAAGTCAGCCGCCAAGTCACTCTGCAAATTGGTGTCCTTAATGAAGCCCAGTTCACTA; ACGCTGCCAACGCACAACCACCAACAATGTGCACCACAACTGTCACTGTGAAAATTAAAGACAGAGATGA; GGGCCCTGAGTGCCAGCCACCAGTCAAAGTCATTCAGAGCAAAGATGGACTGCCAGCGGGCCAAGAGCTC; CTGGGCTACAAAGCGGTGGACCCAGAGACAAGCAGTGGTGAAGGCTTAAGGTATGAGATGGTAGGAGATG; AAGATAATTGGTTTGAGATTAACAAAATCACCGGAGACTTGAGAACTGTGAAAGTACTGGACAGAGAGTC; GAAGTTTGTGAAAAACAACCAGTACAATATTTCAGTAGTGGCGACAGATACAGCTGGCCGATCTTGCACA; GGAACACTGGTAGTTCTTCTGGAAGATTTTAATGACCACCCACCACAGATTGACAAGGAAGTGACCATTT; GTCAGCAGGAGAAGGATTTTGCTGTTTTGGAACCTATAGATTTAGATGGGCCAGATAATGGTCCACCTTT; TCAGTTCCTTCTGGATAATTCTTCCAGCAAACTTTGGACTCTAGAATCACAGGATGGTAAACGTGCCATT; CTTCGTCAACGACACAATCTTAATTATAACTATTATTCTGTGCCAATCCAAATACAAGACAGGCATGGTT; TTTCCGCAAAACATGTATTATCAGTGCGAGTGTGTGACTGTACAACTCCAACAGAATGTAGAATGGCTGT; TAAGGAAGAGAGGGATGCTAAACCAAATATAATCCTTGGGAAATGGGCCATTCTTGCCATGGTCCTGGGC; TCTGCATTGCTACTGTGCATTTTGTTTACATGCTTCTGTGTGACCACTACCAAGAGAACAGTCAAGAAAT; GCTTCCCAGATGATGTAGCTCAGCAAAATTTAATTGTATCAAATACTGAAGGACCAGGAGAAGAAGTGAC; GGAAGCCAATATTAGACTCCCCACACAGACAGCCAACATCTGTGATACAAGCATGTCTGTTGGGACTCTT; GGTGGTCAAGGAATCAAAACACAGCAAAGTTTTGAGATGGTCAAAGGAGGCCACACCTTGGAGTCCCACA; AGGGAGGAGTGCTAGGAGCAGCAGAGCCTGGCCGATATGCTTACACAGACTGGCAAACTTTCACTCAACC; TCGGCTTGGTGAAGAATCCATTAGAGGACACACTCTGATTAAAAATTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013504
• Insert Size: 2499 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013504.5, NP_038532.3
• RefSeq Size: 5007 bp
• RefSeq ORF: 2499 bp
• Locus ID: 13505
• Cytogenetics: 18 A2
• Summary: This gene encodes a member of the cadherin family of proteins that mediates adhesion in desmosomes. The encoded preproprotein undergoes proteolytic processing to generate the mature, functional protein. Mice lacking the encoded protein exhibit epidermal fragility together with defects of epidermal barrier and differentiation. The neonatal mice lacking the encoded protein exhibit epidermal lesions and older mice develop chronic dermatitis. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (2, also known as Dsc1b) contains an alternate exon in the 3' end compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.