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Stt3b (NM_024222) Mouse Untagged Clone

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SKU
MC221873
Stt3b (untagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $830.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Stt3b
Synonyms 1300006C19Rik; Simp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221873 representing NM_024222
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGCGGCCTCA
TGGCTCTGGGGAACAGCCGCCACGGGCACCATGGGCCCGGAACCCAGAGCGCGTCCAGGGCGGCGGCGCC
GAAGCCGGGGCCCCCCGCGGGGCTGTCCGGGGGCTTGTCGCAGCCGGCCGGGTGGCAGTCGTTGCTCTCC
TTCACCATCCTCTTCCTGGCCTGGCTGGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCGAGA
GCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCATGGATTCTA
TGAGTTTCTAAATTGGTTTGATGAAAGAGCATGGTACCCACTGGGAAGAATAGTGGGTGGCACCGTTTAC
CCAGGGTTGATGATAACAGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACAGTTCACATAA
GAGATGTGTGTGTATTCCTTGCACCAACTTTTAGCGGCCTTACATCCATATCTACGTTCCTGCTAACTAG
AGAACTGTGGAACCAAGGAGCAGGACTTCTAGCTGCTTGCTTCATTGCTATCGTACCAGGGTACATATCT
CGGTCAGTGGCGGGATCCTTTGATAATGAAGGCATTGCCATTTTTGCGCTTCAGTTCACTTACTACTTAT
GGGTAAAGTCTGTGAAGACCGGGTCTGTGTTCTGGACAATGTGCTGCTGCTTGTCATATTTCTACATGGT
CTCTGCGTGGGGAGGTTATGTGTTCATCATCAACCTCATCCCTCTCCATGTGTTTGTGTTGCTGCTGATG
CAGAGGTACAGCAAGAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATATTATCCATGC
AGATACCTTTTGTGGGATTTCAGCCAATCAGAACAAGCGAGCACATGGCAGCTGCAGGTGTCTTTGCGCT
GCTGCAAGCTTACGCTTTTTTGCAGTATCTGAGAGACCGGTTGACAAAACAGGAGTTCCAGACCCTTTTC
TTTTTGGGTGTCTCACTAGCTGCAGGCGCTGTGTTCCTTAGTGTCATCTATCTGACATACACAGGTTATA
TTGCACCATGGAGTGGCAGGTTTTATTCACTATGGGATACTGGGTATGCAAAAATACACATTCCAATTAT
TGCATCAGTGTCTGAACATCAGCCTACGACATGGGTGTCTTTCTTCTTTGATCTACATATTCTTGTATGT
ACCTTCCCAGCAGGCCTATGGTTCTGCATCAAAAATATCAACGATGAAAGAGTATTTGTCGCTCTGTATG
CGATCAGTGCTGTCTACTTTGCCGGAGTGATGGTGCGGCTGATGCTGACTCTGACCCCGGTCGTCTGCAT
GCTGTCGGCCATCGCCTTCTCCAATGTTTTTGAGCACTATTTGGGGGATGACATGAAAAGGGAAAACCCA
CCTGTGGAGGACAGCAGTGATGAGGATGACAAAAGAAACCCAGGAAACTTGTATGACAAGGCAGGTAAAG
TGAGGAAGCATGTGACAGAGCAAGAGAAACCTGAAGAGGGCTTGGGCCCCAACATCAAAAGCATTGTGAC
CATGCTGATGCTCATGCTCCTGATGATGTTCGCGGTCCACTGCACGTGGGTCACAAGCAACGCCTACTCC
AGTCCAAGTGTGGTCCTTGCCTCCTACAATCATGATGGTACCAGGAATATATTAGATGATTTTAGAGAAG
CGTACTTTTGGCTGAGACAAAACACGGATGAACACGCCCGGGTCATGTCGTGGTGGGACTACGGCTATCA
GATTGCTGGCATGGCCAACAGGACCACTCTGGTGGATAACAACACCTGGAACAACAGCCACATCGCACTG
GTCGGAAAAGCTATGTCTTCCAATGAAACGGCCGCCTATAAAATCATGAGGTCCCTTGATGTCGATTATG
TGTTGGTTATTTTCGGAGGAGTGATTGGCTATTCCGGGGACGATATCAACAAGTTCCTCTGGATGGTCAG
GATAGCTGAAGGGGAGCATCCCAAAGACATCCGGGAAGGTGACTATTTCACCCAGCAGGGAGAGTTCCGA
GTAGACAAAGCTGGGTCTCCTACTCTGTTAAACTGCCTTATGTATAAAATGTCATACTACAGATTTGGAG
AAATGCAGCTAGATTTTCGCACTCCCCCAGGCTTTGACCGAACACGTAATGCTGAGATTGGAAATAAAGA
CATTAAATTCAAGCATTTGGAGGAAGCTTTTACATCAGAGCACTGGCTTGTCAGGATATATAAAGTGAAA
GCACCTGACAACAGGGAGACACTAGGTCACAAACCTCGAGTCACCAACATCGTCCCCAAACAGAAGTATT
TGTCAAAGAAGACTACTAAAAGGAAGCGTGGCTACGTTAAAAATAAGCTAGTGTTTAAGAAAGGCAAGAA
GACCTCTAAGAAGACTGTTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_024222
Insert Size 2472 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024222.2, NP_077184.2
RefSeq Size 4236 bp
RefSeq ORF 2472 bp
Locus ID 68292
UniProt ID Q3TDQ1
Cytogenetics 9 F3
Summary Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_024222" in other vectors (4)
SKU Description Size Price
MG210829 Stt3b (tGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) 10 ug
$954.00
MR210829 Stt3b (Myc-DDK-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) 10 ug
$589.00 $754.00
MR210829L3 Lenti ORF clone of Stt3b (Myc-DDK-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) 10 ug
$1,054.00
MR210829L4 Lenti ORF clone of Stt3b (mGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (Stt3b) 10 ug
$1,054.00

file-alt Citations

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